Partitioning Studies of Alpha-amylase in Aqueous Two Phase System Composing of Polyethylene Glycol and Tri-Sodium Citrate
Subject Areas : Microbiologyمریم Karimi 1 , N. Hadidi 2 , Gh. Pazuki 3 , Sh. Abdolrahimi 4
1 - M. Sc. Graduated in Chemical Engineering, Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
2 - Assistant Professor of the Department of Clinical Research and Electronic Microscope, Pasteur Institute of Iran, Tehran, Iran.
3 - Associate Professor of the Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
4 - Ph. D. Graduated in Chemical Engineering, Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
Keywords: &, alpha, -Amylase, Aqueous Two Phase System, Poly Ethylene Glycol, Partitioning, Tri Sodium Citrate,
Abstract :
Introduction: One of the scenario for separation of biotechnology products is the application of aqueous two-phase systems (ATPS) rather than traditional extraction by using solvent. The ATPSs are applicable method for separation and purification of biomolecules mixture. The ATPS can be applied for protein purification of biomolecules from suspension broth. The goal of the present research is the study of partitioning of -amylase in polymer-salt aqueous two-phase system composed of polyethylene glycol with molecular weight of 1000 and tri-sodium citrate salt as a biocompatible salt. Materials and Methods: The aim of this research is to study partitioning of α-amylase enzyme in an aqueous two phase system. The system consists of polyethylene glycol (PEG) with molecular weight of 1000 and tri-sodium citrate that is a biocompatible salt. Results: The results for partitioning of enzyme in ATPS has been modeled and statistically analyzed; and indicated that the partition coefficient of -amylase in the system has the maximum amount of 2.681. Conclusion: In this research it has been proved that ATPS components concentration has an effect on the enzyme partition coefficient. Using experimental results, -amylase has a great tendency to be placed in the PEG rich phase.
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