The Antimicrobial Effect of Chitosan Nano Particles Film on Two Species of Food Bacteria

Subject Areas : Microbiology

P. Rezaee ¹ , روحا Kasra Kermanshahi ²

1 - کارشناس ارشد میکروبیولوژی، گروه میکروبیولوژی، دانشکده زیست شناسی، دانشگاه الزهرا(س)، تهران، ایران
2 - استاد گروه میکروبیولوژی، دانشکده زیست شناسی، دانشگاه الزهرا(س)، تهران، ایران

Received: 2014-01-19 Accepted : 2014-06-09 Published : 2015-06-22

Keywords: Chitosan film, Food Antimicrobial Packaging, Listeria monocytogenes, Nanoparticle, Staphylococcus aureus,

Abstract :

Introduction: Unbiodegradable materials used in food packaging and the microbial contaminants derived from this might create problems in our society. The application of biodegradable material that might have antimicrobial activity is desirable and is considered a priority for the researchers. Chitosan is a biopolymer that eliminates these problems. Materials and Methods: In this study, chitosan nanoparticles and chitosan films were prepared and physical and chemical characteristics of these were determined and the antimicrobial effects against Staphylococcus aureus PTCC 1431 and Listeria monocytogenesusing different methods were assessed. Results: The size of chitosan nanoparticle was 273 nm with MIC and MBC of 3.5  for these bacteria. Synthezied chitosan film with 43.69 ± 2.4 mm of thickness, 1% of moisture content and 52% film solubility caused a reduction of 100% in the number of food pathogenic bacteria in 24 hoursat 30°C. Conclusion: Since chitosan is a biodegradable polymer and has antimicrobial activity in the form of chitosan nanoparticle and film against food pathogenic bacteria (Staphylococcus aureus PTCC 1431 and Listeria monocytogenes), it might be employed in the packaging industry particularly food.