The anticancer and antibacterial properties of Zingiber officinale (ginger) extracts on cell viability of Cancer cells(AGS) and some Pathogenic bacteria
Subject Areas : MicrobiologyFatemeh Abdollahi 1 , Faranak Aali 2 , Ali Sharifzadeh 3 *
1 - Department of Microbiology, Faculty of Sciences , , Shahrekord Branch, Islamic Azad University, Shahrekord, I.R. Iran
2 - Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, I.R. Iran
3 - Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, I.R. Iran.
Keywords: Antimicrobe, anticancer, Zingiber officinale, Extract,
Abstract :
Introduction: Today Ginger in fresh and dried form is used as a spice around the world.Ginger with a scientific name of Zingiber officinale, is a herbal medicine. It is widely used inthe traditional medicine and utilised as a dietary supplement. The aim of this study is todetermine the antibacterial and anticancer properties and activities of hydroalcoholic extractof ginger.Materials and Methods: The cell lines were grown in RPMI supplemented with 10% FBS,1% penicillin and streptomycin. The cells were allowed to incubate at 37ºC in an atmospherethat contained 5% CO2 and 95% humidity. The extracts were also used with MIC onKlebciella Pneumoniae, E. Coli, Pseudomonas aeroginosa and Staphylococcus aureus. Thestandard MTT assay was performed to estimate cell viability after treatment by Zingiberextracts. We examined cytotoxicity effects of different concentrations the (250, 500, 750,1000, 1250, 1500, 1750, 2000, 1250, 2500, 2750 μg/ml) of Zingiber extracts on AGS celllines were examined.Results: Based on the findings of this Study, these extracts at different concentrations hadinhibitory effect on Klebciella Pneumoniae, E.Coli, and Staphylococcus aureus. Also Theresults of the MTT assay showed that the Zingiber extracts based on time and concentrationhad anticancer activities.Conclusion: Based on our results and findings it might be suggested that ginger extract withoptimum concentrations might be employed in food formulations to inhibit pathogenicbacteria and cancer cell lines.
Adams-Sapper, S., Nolen, S., Donzelli, G.F., Lal, M., Chen, K. & Justo da Silva L.H. (2015). Rapid induction of high-level carbapenem resistance in heteroresistant KPC-producing Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 59(6), 3281-3289.
Agrawal, N., Majee, C. & Chakraborthy, G.S. ) 2012 . (Herbs as Anticancer Drugs. International. International Journal of PharmTech Research, 4(3), 1142-1153.
Asmarian, N., Jafari-Koshki, T., Soleimani, A. & Taghi Ayatollahi, S.M. (2016). Area-to-Area Poisson Kriging and Spatial Bayesian Analysis in Mapping of Gastric Cancer Incidence in Iran. Asian Pacific Journal of Cancer Prevention, 17(10), 4587-4590.
Avci, G.A., Avci, E., Ozluk, G. & Coskun Cevher, S. (2020). Antimicrobial and Antioxidant Activities of Zingiber officinale (Ginger) and Alpinia officinarum (Galangal). Hittite Journal of Science & Engineering, 7(1), 45-49.
Dadfar, F., Hosseini, S. E. & Bahaoddini, A. (2014). A review of phytochemical, pharmacological and physiological properties of ginger (zingiber officinale). Clinical Excellence, 3 (1), 72-86. [In Persian] [1]
Ficker, C.E., Arnason, J.T., Vindas, P.S., Alvarez, L.P., Akpagana, K., GbA assor, M., De souza, C. & Smith, M.L. (2003). Inhibition of human pathogenic fungi by ethnobotanically selected plant extracts. Mycoses, 46(1), 29–37.
Kafash-Farkhad, N., Asadi-Samani, M. & Khaledifar, B. (2013). A review on secondary metabolites and /pharmacological effects of Prangos ferulacea (L.) Lindl. Journal of Shahrekord University of Medical Sciences, 15 (3), 98-108.
Keikha, M. & Rawa, M. (2017). Trend of antibiotic resistance of Escherichia coli strains isolated from urinary tract infections in outpatients referred to Nabi Akram Hospital in Zahedan. Journal of Paramedical Sciences and Rehabilitation Sciences, 6 (4), 73-78. [In Persian]
Lu W.D., Zuo, Y., Xu, Z. & Zhang, M. (2015). MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer. World Journal of Gastroenterology, 21(15), 4564-4573.
Mathai, K., Anand, S. & Aravind, A., (2017). Antimicrobial Effect of Ginger, Garlic, Honey, and Lemon Extracts on Streptococcus mutans. The Journal of Contemporary Dental Practice, 18(11), 1004-1008.
Moheghi, N., Afshari, J. T. & Brook, A. (2011). The Cytotoxic Effect of Zingiber Afficinale in Breast Cancer (MCF7) Cell Line. Internal Medicine Today, 17 (3), 28-34. [In Persian]
Omoya, F.O. & Akharaiyi, F.C. (2011). Mixture of honey and ginger extract for antibacterial assessment on some clinical isolates. International Journal on Pharmaceutical and Biomedical Research, 2(1), 39-47.
Rajabpour, M., Arabestani, M. R., Yousefi Mashof, R. & Alikhani, M. Y. (2013). MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iranian Journal of Medical Microbiology, 7 (3), 18-25. [In Persian]
Ramakrishnan, R. (2013). Anticancer properties of zingiber officinale ginger: A review. International Journal of Medicine and Pharmaceutical Sciences, 3(5), 11-20.
Ranjbar, M., Nedainia, R., Goli, M., Manian, M., Marci, M. R., Faizi, M. & Kargaran, N. (2016). Study of Escherichia coli strains (E.coli) obtained from traditional ice cream based on protein profile in Isfahan city. Biology of Microorganisms, 5 (17), 171-184.
Schlecht, L.M., Peters, B.M. & Krom, B.P. (2015). Systemic Staphylococcus aureus infection mediated by Candida albicans hyphal invasion of mucosal tissue. Microbiology (Reading), 161(1), 168-181.
Tabatabai, F., Fallah, F., Behbahani, B.A., Vasiee, A. & Mortazavi, S.A. (2019). Identification of Chemical Compounds, Antioxidant Potential, Phenolic Content and Evaluation of Inhibitory and Bactericidal/Fungicidal Effects of Ginger Essential Oil on Some Pathogenic Microorganisms in Vitro. Qom University of Medical Sciences, 13(3), 62-50. [In Persian]
Terpinc, P., Bezjak, M. & Abramovic, H. (2009). A kinetic model for evaluation of the antioxidant activity of several Rosemary extracts. Food Chemistry, 115(2), 740-744.
Zia, M., Beheshti, S., Khalkhali, H. & Saffari, S. (2013). Detection of antibiotic resistance in different strains of staphylococcus aureus using Disc diffusion Agar. RJMS, 20 (111), 70-78. [In Persian]
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Adams-Sapper, S., Nolen, S., Donzelli, G.F., Lal, M., Chen, K. & Justo da Silva L.H. (2015). Rapid induction of high-level carbapenem resistance in heteroresistant KPC-producing Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 59(6), 3281-3289.
Agrawal, N., Majee, C. & Chakraborthy, G.S. ) 2012 . (Herbs as Anticancer Drugs. International. International Journal of PharmTech Research, 4(3), 1142-1153.
Asmarian, N., Jafari-Koshki, T., Soleimani, A. & Taghi Ayatollahi, S.M. (2016). Area-to-Area Poisson Kriging and Spatial Bayesian Analysis in Mapping of Gastric Cancer Incidence in Iran. Asian Pacific Journal of Cancer Prevention, 17(10), 4587-4590.
Avci, G.A., Avci, E., Ozluk, G. & Coskun Cevher, S. (2020). Antimicrobial and Antioxidant Activities of Zingiber officinale (Ginger) and Alpinia officinarum (Galangal). Hittite Journal of Science & Engineering, 7(1), 45-49.
Dadfar, F., Hosseini, S. E. & Bahaoddini, A. (2014). A review of phytochemical, pharmacological and physiological properties of ginger (zingiber officinale). Clinical Excellence, 3 (1), 72-86. [In Persian] [1]
Ficker, C.E., Arnason, J.T., Vindas, P.S., Alvarez, L.P., Akpagana, K., GbA assor, M., De souza, C. & Smith, M.L. (2003). Inhibition of human pathogenic fungi by ethnobotanically selected plant extracts. Mycoses, 46(1), 29–37.
Kafash-Farkhad, N., Asadi-Samani, M. & Khaledifar, B. (2013). A review on secondary metabolites and /pharmacological effects of Prangos ferulacea (L.) Lindl. Journal of Shahrekord University of Medical Sciences, 15 (3), 98-108.
Keikha, M. & Rawa, M. (2017). Trend of antibiotic resistance of Escherichia coli strains isolated from urinary tract infections in outpatients referred to Nabi Akram Hospital in Zahedan. Journal of Paramedical Sciences and Rehabilitation Sciences, 6 (4), 73-78. [In Persian]
Lu W.D., Zuo, Y., Xu, Z. & Zhang, M. (2015). MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer. World Journal of Gastroenterology, 21(15), 4564-4573.
Mathai, K., Anand, S. & Aravind, A., (2017). Antimicrobial Effect of Ginger, Garlic, Honey, and Lemon Extracts on Streptococcus mutans. The Journal of Contemporary Dental Practice, 18(11), 1004-1008.
Moheghi, N., Afshari, J. T. & Brook, A. (2011). The Cytotoxic Effect of Zingiber Afficinale in Breast Cancer (MCF7) Cell Line. Internal Medicine Today, 17 (3), 28-34. [In Persian]
Omoya, F.O. & Akharaiyi, F.C. (2011). Mixture of honey and ginger extract for antibacterial assessment on some clinical isolates. International Journal on Pharmaceutical and Biomedical Research, 2(1), 39-47.
Rajabpour, M., Arabestani, M. R., Yousefi Mashof, R. & Alikhani, M. Y. (2013). MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iranian Journal of Medical Microbiology, 7 (3), 18-25. [In Persian]
Ramakrishnan, R. (2013). Anticancer properties of zingiber officinale ginger: A review. International Journal of Medicine and Pharmaceutical Sciences, 3(5), 11-20.
Ranjbar, M., Nedainia, R., Goli, M., Manian, M., Marci, M. R., Faizi, M. & Kargaran, N. (2016). Study of Escherichia coli strains (E.coli) obtained from traditional ice cream based on protein profile in Isfahan city. Biology of Microorganisms, 5 (17), 171-184.
Schlecht, L.M., Peters, B.M. & Krom, B.P. (2015). Systemic Staphylococcus aureus infection mediated by Candida albicans hyphal invasion of mucosal tissue. Microbiology (Reading), 161(1), 168-181.
Tabatabai, F., Fallah, F., Behbahani, B.A., Vasiee, A. & Mortazavi, S.A. (2019). Identification of Chemical Compounds, Antioxidant Potential, Phenolic Content and Evaluation of Inhibitory and Bactericidal/Fungicidal Effects of Ginger Essential Oil on Some Pathogenic Microorganisms in Vitro. Qom University of Medical Sciences, 13(3), 62-50. [In Persian]
Terpinc, P., Bezjak, M. & Abramovic, H. (2009). A kinetic model for evaluation of the antioxidant activity of several Rosemary extracts. Food Chemistry, 115(2), 740-744.
Zia, M., Beheshti, S., Khalkhali, H. & Saffari, S. (2013). Detection of antibiotic resistance in different strains of staphylococcus aureus using Disc diffusion Agar. RJMS, 20 (111), 70-78. [In Persian]