Antibacterial Properties of Lactobacillus acidophilus and Bacillus coagulans cell-free Culture Medium Against Resistant Pathogens.
Subject Areas :Ali Kazemi 1 , Nader Habibi 2 *
1 - MS.c Graduated of Food Science and Technology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
2 - Department of Food Science and Technology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran.
Keywords: Lactobacillus acidophilus, Bacillus coagulans, Escherichia coli, Staphylococcus aureus,
Abstract :
Recently, the use of probiotics in controlling pathogenic bacteria has become very important. Inaddition, they have useful properties to maintain the health of the body. The aim of this study was to investigate the antibacterial properties of cell-free culture medium of two probiotic bacteria Lactobacillus acidophilus and Bacillus coagulans against two foodborne pathogens Escherichia coli and Staphylococcus aureus. Pathogenic bacteria from the samples of raw cow's milk distributed in Sanandaj city using special and differential isolation media, then their antibiotic sensitivity was checked by disk diffusion method and multi-drug resistant isolates were used to perform tests laboratory were selected. After culturing probiotic bacteria, their cell-free liquid was used to check antibacterial properties by MIC and MBC methods. Also, the interaction between two probiotics was investigated using the checkerboard method and determining the FICi index. From 180 samples taken, 15 isolates of Escherichia coli (8.33%) and 19 isolates of Staphylococcus aureus (10.55%) were isolated. Based on the result of the antibiotic sensitivity test, 10 isolates were selected for each of the pathogenic bacteria.he results showed that the culture medium grown by both probiotic bacteria has antibacterial power against both pathogenic bacteria, but the simultaneous examination of these two probiotics showed a synergistic effect. Staphylococcus aureus bacteria was more sensitive than Escherichia coli. MIC and MBC values ranged from 2.5 to 160 μl/ml. Therefore, the cell-free culture medium of Lactobacillus acidophilus and Bacillus coagulans can be introduced as a new biological compound with antibacterial properties.
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