Effect of Lactobacillus plantarum on microbial population of Iranian fermented pickle
Subject Areas :
Food Science and Technology
زهرا Nilchian
1
,
S.H Razavi
2
,
ابراهیم Rahimi
3
1 - Msc student of Food Science and Technology, School of Agriculture, Shahrekord branch, Islamic Azad University, Shahrekord , Iran.
2 - Associate professor of Food Science and Engineering Department, Faculty of Biosystem Engineering, University of Tehran, Tehran, Iran
3 - Associate Professor of Food Sciences and Technology Department, School of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Received: 2012-06-09
Accepted : 2013-04-09
Published : 2012-08-22
Keywords:
Lactobacillus plantarum,
Microbial population,
Fermented pickle,
Abstract :
Pickle fermentation is a natural process in which microorganisms could affect the quality of the product. Amongst, Lactobacillus plantarum strains that improve the quality of fermented pickle. In this study, in order to produce safe and desirable fermented pickle, local cucumbers were immersed in 5% and 7% (w/v) brine solutions. The samples were inoculated with 0, 4 ´106, 4 ´107 and 6 ´108 cfu/ml of L. plantarum. The samples were kept at ambient temperature for 15 days followed by 30 days storage at 4 °C and 25 °C. The bacterial populations were analyzed during the aforementioned stages of fermentation and storage. According to the results, the maximum quantity (8.57 log10 cfu/ml) of L. plantarum was observed in the 9 day of fermentation and in the samples inoculated with 6 ´108 cfu/ml and kept in 5% brine solution. Moreover, compared to the other samples, the population of yeasts and aerobic mesophilic count were the least in the samples inoculated with 6 ´108 cfu/ml of L. plantarum and stored in 7% brine solution. During the storage period, the population of L. plantarum, yeasts and aerobic mesophilic in the samples stored at 25 °C were higher than the samples stored at 4 °C. Results revealed that in the 30 day of storage, the highest load (5.47 log10 cfu/ml) of L. plantarum was observed in the samples kept in 7% brine solution at 4 °C. It was concluded that providing the condition that favors the appropriate growth of L. plantarum could help to hinder the growth of undesirable organisms in fermented pickle.
References:
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Bautista-Gallego, J., Arroyo-Lopez, F.N., Duran-Quintana, M.C. and Garrido-Fernandez, A. (2010). Fermentation profiles of Manzanilla-Alorena cracked green table olives in different chloride salt mixtures. Food Microbiology, 27: 403-412.
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Chammem, N., Kachouri, M., Mejri, M., Peres, C., Boudabous, A. and Hamdi, M. (2005).Combined effect of alkali pretreatment and sodium chloride addition on the olive fermentation process. Bioresource Technology, 96: 1311-1316.
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Charalampopoulos, D. and Pandiella, S.S. (2010). Survival of human derived Lactobacillus plantarum in fermented cereal extracts during refrigerated storage. LWT-Food Science and Technology, 43: 431-435.
Gardner, J.N., Savard, T., Obermeier, P., Caldwell, G. and Champagne, C.P.(2001).Selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. International Journal of Food Microbiology, 64: 261-275.
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Leal-Sanchez, M.V., Ruiz-Barba, J.L., Sanchez, A.H., Rejano, L., Jimenez-Dıaz, R. and Garrido, A. (2003).Fermentation profile and optimization of green olive fermentation using Lactobacillus plantarum LPCO10 as a starter culture. Food Microbiology, 20: 421-430.
Lucke, F.K. (2000). Utilization of microbes to process and preserve meats. Meat Science, 56: 105-115.
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Romick, T.L. (1994). Biocontrol of Listeria monocytogenes, a psychrotrophic pathogen model, in low salt, non-acidified, refrigerated vegetable products. PhD Diss, NC State University.Raleigh, NC.
Sanchez, I., Palop, L. and Ballesteros, C. (2000). Biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'Almagro' eggplants. International Journal of Food Microbiology, 59: 9-17.
Singh, A.K. and Ramesh, A. (2008). Succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: Insights from a PCR-based approach. Food Microbiology, 25: 278-287.
Tamang, J.P., Tamang, B., Schillinger, U., Guigas, C. and Holzapfel, W.H.(2009). Functional properties of lactic acid bacteria isolated from ethnic fermented vegetables of the Himalayas. International Journal of Food Microbiology, 135: 28-33.
Todorov, S.D. and Dicks,L.M.T. (2005). Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria. Enzyme and Microbial Technology, 36: 318-326.
Tsai, C.C., Lai, C.H., Yu, B. and Tsen, H.Y. (2010). Use of PCR primers and probes based on the 23S rRNA and internal transcription spacer (ITS) gene sequence for the detection and enumerization of Lactobacillus acidophilus and Lactobacillus plantarum in feed supplements. Anaerobe, 16: 270-277.
Tsapatsaris, S. and Kotzekidou, P. (2004). Application of central composite design and response surface methodology to the fermentation of olive juice by Lactobacillus plantarum and Debaryomyces hansenii. International Journal of Food Microbiology, 95: 157-168.
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Abriouel, H., Benomar, N., Lucas, R. and Galvez, R. (2011).Culture-independent study of the diversity of microbial populations in brines during fermentation of naturally-fermented Alorena green table olives. International Journal of Food Microbiology, 144: 487-496.
Bautista-Gallego, J., Arroyo-Lopez, F.N., Duran-Quintana, M.C. and Garrido-Fernandez, A. (2010). Fermentation profiles of Manzanilla-Alorena cracked green table olives in different chloride salt mixtures. Food Microbiology, 27: 403-412.
Caggia, C., Randazzo, C.L., Di Salvo, M., Romeo, F. and Giudici, P. (2004).Occurrence of Listeria monocytogenes in green table olives. Journal of Food Protection, 67: 2189-2194.
Castro, A., Montano, A., Casado, F.J., Sanchez, A.H. and Rejano, L. (2002). Utilization of Enterococcus casseliflavus and Lactobacillus pentosus as starter cultures for Spanish-style green olive fermentation. Food Microbiol, 19: 637-644.
Chammem, N., Kachouri, M., Mejri, M., Peres, C., Boudabous, A. and Hamdi, M. (2005).Combined effect of alkali pretreatment and sodium chloride addition on the olive fermentation process. Bioresource Technology, 96: 1311-1316.
Charalampopoulos, D., Pandiella, S.S. and Webb, C. (2003). Evaluation of the effect of malt, wheat and barley extracts on the viability of potentially probiotic lactic acid bacteria under acidic conditions. International Journal of Food Microbiology,82(2): 133-141.
Charalampopoulos, D. and Pandiella, S.S. (2010). Survival of human derived Lactobacillus plantarum in fermented cereal extracts during refrigerated storage. LWT-Food Science and Technology, 43: 431-435.
Gardner, J.N., Savard, T., Obermeier, P., Caldwell, G. and Champagne, C.P.(2001).Selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. International Journal of Food Microbiology, 64: 261-275.
Garrido Fernandez, A., Garcia Garcia, P. and Brenes Balbuena, M. (1995).Olive fermentations.In Rehm, H.J. and Reed, G. (Editors.). Biotechnology: Enzymes, biomass, food and feed. New York, VCH, pp. 593-627.
Garrido-Fernandez, A., Fernandez Diez, M.J. and Adams, M.R. (1997). Table Olives: Production and Processing. Chapman and Hall, London.
Kabadjova, P., Dousset, X., Le Cam, V. and Prevost, H. (2002).Differentiation of closely related Carnobacterium food isolates based on 16S-23S ribosomal DNA intergenic spacer region polymorphism. Applied and Environmental Microbiology, 68: 5358-5366.
Leal-Sanchez, M.V., Ruiz-Barba, J.L., Sanchez, A.H., Rejano, L., Jimenez-Dıaz, R. and Garrido, A. (2003).Fermentation profile and optimization of green olive fermentation using Lactobacillus plantarum LPCO10 as a starter culture. Food Microbiology, 20: 421-430.
Lucke, F.K. (2000). Utilization of microbes to process and preserve meats. Meat Science, 56: 105-115.
Milesi, M.M., McSweeney, P.L.H. and Hynes, E.R. (2008). Viability and contribution to proteolysis of an adjunct culture of Lactobacillus plantarum in two model cheese systems: cheddar cheese-type and soft-cheese type. Journal of Applied Microbiology, 105: 884-892.
Ozay, G. andBorcakli, M. (1995).Effect of brine replacement and salt concentration on the fermentation of naturally black olives. Food Research International, 28: 553-559.
Panagou, E.Z, Schillinger, U., Franz, C.M.A.P. and Nychas, G.J.E. (2008). Microbiological and biochemical profile of cv. Conservoleagreen olives processed by the Spanish-method. Food Microbiol, 23: 199-204.
Paramithiotis, S., Hondrodimou, O.L. and Drosinos, E.H.(2010). Development of the microbial community during spontaneous cauliflower fermentation. Food Research International, 43: 1098-1103.
Penas, E., Frias, J., Gomez, R. and Vidal-Valverde, C. (2010). High hydrostatic pressure can improve the microbial quality of sauerkraut during storage, Spain. Food Control, 21: 524-528.
Plengvidhya, V., Breidt Jr., F., Lu, Z. and Fleming, H.P. (2007).DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations. Applied and Environmental Microbiology, 73: 7697-7702.
Rodriguez-Gomez, F., Arroyo-Lopez, F.N., Lopez-Lopez, A., Bautista-Gallego, J. and Garrido-Fernandez, A. (2010). Lipolytic activity of the yeast species associated with the fermentation/storage phase of ripe olive processing. Food Microbiology, 27: 604-612.
Romick, T.L. (1994). Biocontrol of Listeria monocytogenes, a psychrotrophic pathogen model, in low salt, non-acidified, refrigerated vegetable products. PhD Diss, NC State University.Raleigh, NC.
Sanchez, I., Palop, L. and Ballesteros, C. (2000). Biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'Almagro' eggplants. International Journal of Food Microbiology, 59: 9-17.
Singh, A.K. and Ramesh, A. (2008). Succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: Insights from a PCR-based approach. Food Microbiology, 25: 278-287.
Tamang, J.P., Tamang, B., Schillinger, U., Guigas, C. and Holzapfel, W.H.(2009). Functional properties of lactic acid bacteria isolated from ethnic fermented vegetables of the Himalayas. International Journal of Food Microbiology, 135: 28-33.
Todorov, S.D. and Dicks,L.M.T. (2005). Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria. Enzyme and Microbial Technology, 36: 318-326.
Tsai, C.C., Lai, C.H., Yu, B. and Tsen, H.Y. (2010). Use of PCR primers and probes based on the 23S rRNA and internal transcription spacer (ITS) gene sequence for the detection and enumerization of Lactobacillus acidophilus and Lactobacillus plantarum in feed supplements. Anaerobe, 16: 270-277.
Tsapatsaris, S. and Kotzekidou, P. (2004). Application of central composite design and response surface methodology to the fermentation of olive juice by Lactobacillus plantarum and Debaryomyces hansenii. International Journal of Food Microbiology, 95: 157-168.
