Antibacterial combined effects of nisin and onion essential oil under different concentration of NaCl and pH against Listeria monocytogenes in vitro

Subject Areas : Food Science and Technology

S.M Razavi Rohani ¹ , مهران Moradi ² , تورج Mehdizadeh ³

1 - Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. - Department of Medicinal and Industrial Plant, Institute of Biotechnology, Urmia University, Urmia, Iran
2 - Department of Food Science and Technology, Ahar Faculty of Agriculture, Tabriz University, Tabriz, Iran
3 - Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Received: 2011-11-12 Accepted : 2011-12-20 Published : 2011-11-22

Keywords: Combination, L .monocytogenes, Nisin, Oinion essential oil,

Abstract :

   In the present study, the anti-listeria activity of onion (Allium cepa L.) essential oil (EO), nisin as well as their combination at various pH values (4.8, 5.8 and 6.8) and different NaCl concentrations (0, 0.5, 2.5 and 4.5%) was investigated against Listeria monocytogenes by microtiter plates at 30°C. Minimum inhibitory concentrations (MICs) were assessed for the nisin, onion EO as well as their combination. Subsequently, fractional inhibitory concentration (FIC) was evaluated under different pH and NaCl concentrations. Both nisin and EQ showed significant antimicrobial effects against L.monocytogenes. Moreover, the nisin and onion EO exhibited MICs of 12.5 IU mL-1 and 125 μg mL-1, respectively. FICs of the nisin and onion EO alone and in combined form, along with various combinations of pH and NaCl concentration, showed clearly anti-listeria effect as synergistic, additional or indifference. Regardless of NaCl concentrations, the anti-listeria activity of both agents was strongly influenced by pH. Moreover, regardless of pH value, the growth of the bacterium was also affected by increasing of NaCl concentrations. It was concluded that, pH value of 4.8 and NaCl concentration of 4.5% had significant anti-listeria effect. In addition, it was found that, the effect of NaCl concentrations for each of the combination forms of treatments at various pH was different.Therefore, there are necessity to design and apply a comprehensive predictive model.

References:

• Anonymous. (2009). http://www.answers.com/topic/antimicrobial agent.
  
  
  
  • Appendini, P. and Hotchkiss, J.H. )2002(. Review of antimicrobial food packaging. Innovative Food Science and Emerging Technologies, 3: 113-126.
  
  
  • Bhurinder, S., Bernadette, F. and Martin, R. (2001). Synergistic inhibition of Listeria monocytogenes by nisinsnd garlic extract. Food Microbiology, 15: 133-139.
  
  
  • Boziaris, I.S. and Nychas G.J. (2006). Effect of nisin on growth boundaries of Listeria monocytogenes Scott A, at various temperatures, pH and water activities. Food Microbiology,23: 779-784.
  
  
  • Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods: a review. International Journal of Food Microbiology, 94: 223-53.
  
  
  • Cagri, A., Ustunol, Z. and Ryser, E.T. (2004). Antimicrobial edible films and coatings. Journal of Food Protection, 67: 833-848.
  
  
  • Coma, V. (2008). Bioactive packaging technologies for extended shelf life of meat-based products. Meat Science, 78: 90-103.
  
  
  • Embuscado, M.E. and Huber, K.C. (2009). Edible films and coatings for food applications. Springer, New York, NY, pp. 295-314.
  
  
  • Griffiths, G., Trueman, L., Crowther, T., Thomas, B. and Smith, B. (2002). Onions-A global benefit to health. Phytotherapy Research, 16: 603-615.
  
  
  • Joerger, R.D. (2007). Antimicrobial films for food application: A quantitative analysis of their effectiveness. Packaging Technology and Science, 20: 231-273.
  
  
  • Murdock, C.A., Cleveland, J., Matthews, K.R. and Chikindas, M.L. (2007). Thesynergistic effect of nisin and lactoferrin on the inhibition of Listeria monocytogenes and Escherichia coliO157:H7. Letters in Applied Microbiology, 44: 255-261.
  
  
  • Pol, I.E. and Smid, E.J. (1999). Combined action of nisin and carvacrol on Bacilluscereus and Listeria monocytogenes. Letters in Applied Microbiology, 29: 166-170.
  
  
  • Rayman, M.K., Aris, B. and Hurst, A. (1981). Nisin: a possible alternative or adjunct to nitrite in the preservation of meats, Applied Environmental Microbiology, 41: 375-380.
  
  
  • Razavi Rohani, S.M., Moradi, M., Mehdizadeh, T., Saei Dehkordi, S.S. and Griffiths, M.W. (2011). The effect of nisin and garlic (Allium sativum L.) essential oil separately and in combination on the growth of Listeria monocytogenes. LWT Food Science and Technology,14: 2260-2265.
  
  
  • Ryser, E.T. and Marth, E.H. (2007). Listeria, Listeriosis and Food Safety. 3^th edition, Taylor and Francis, pp. 1-20, 157-214.
  
  
  • Schwalbe, R., Steele-Moore, L. and Goodwin. A.C. (2007). Antimicrobial susceptibility testing protocols. CRC Press, pp. 275-298.
  
  
  • Tajkarimi, M.M., Ibrahim, S.A. and Cliver, D.O. (2010). Antimicrobial herb and spice compounds in food. Food Control, 21: 1199-1218.
  
  
  • Thomas, L.V. and Wimpenny, J.W.T. (1996). Investigation of the effect of combinedvariations in temperature, pH and NaCl concentration on nisin inhibition of Listeriamonocytogenes and Staphylococcus aureus. Applied and Environmental Microbiology, 62: 2006-2012.
  
  
  • Thomas, L.V. and Delves-Broughton, J. (2001(. New advances in the application of the food preservative nisin. Research Advances in Food Science, 2:11-22.
  
  
  • Tiwari, B.K., Valdramidis, V.P., O^' Donnell, C.P., Muthukumarappan, K., Bourke, P. and Cullen, P.J. (2009). Application of natural antimicrobial for food preservation. Journal of Agricultural Food Chemistry, 57: 5987-6000.
  
  
  • Yamazaki, K., Yamamoto, T., kawai, Y. and Inoue, N. (2004). Enhancement of anti Listerial activity of essential oil constitutes by nisin and diglycerol fatty acid ester. Food Microbiology, 21: 283-289.