Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan

Subject Areas : Food Science and Technology

طاهره Narimani ¹ , علیرضا Tarinejad ² , M.A Hejazi ³

1 - MSc Student, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
2 - - Associate Professor, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.
3 - - Agricultural Biotechnology Research Institute of Iran, Branch of North-West and West region, Tabriz, Iran.

Received: 2013-08-19 Accepted : 2014-04-23 Published : 2013-11-22

Keywords: Lactobacilli, Enterococci, Probiotic Bacteria, 16S rRNA gene,

Abstract :

Probiotics are dietary supplements of live microorganisms which when consumed in adequate amounts, can have a beneficial effect on the host. Among all bacteria, lactic acid bacteria are the most common type that has been introduced as probiotics. These bacteria are present in dairy products and produce lactic acid during the fermentation process. The aim of this study was to isolate and identify the probiotics from microbial flora of milk and traditional yogurt in Kaleibar, Heris and Varzaghan areas. In this study, lactic acid bacteria were isolated by culture and identified based on biochemical properties and resistant to stomach acid and bile salts were evaluated. Then, for more accurate identification of the isolates, the 16S rRNA genes of Lactobacilli were amplified with specific primers and the purified PCR product was sent for sequencing. According to our results, 17 strains of Lactobacilli and 6 strains of Enterococci were reported in Kaleibar, Heris and Varzaghan areas which could be a good candidate for further investigation as probiotic.

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