Polymorphism of aroA gene in Staphylococcus aureus isolates from milk and cheese in the city of Tabriz
Subject Areas :
Food Hygiene
A. Hosinkhani
1
,
A.R. Monadi
2
,
J. Shayegh
3
1 - Graduated Student, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Associate Professor of Microbiology, Department of Laboratory Sciences, The School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
3 - Assistant Professor, Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
Received: 2021-01-17
Accepted : 2021-03-17
Published : 2020-12-21
Keywords:
PCR-RFLP,
Staphylococcus aureus,
dairy products,
aroA,
TaqI enzyme,
Abstract :
Due to the pathogenicity of Staphylococcus aureus and its presence in dairy products and the importance of its transmission through dairy products, this study aimed to analyze the aroA gene in S. aureus isolated from cow milk and traditional cheese. For this purpose, 40 S. aureus isolates including 14 traditional cheese isolates, 19 cow milk isolates, and 7 domestic water buffalo milk isolates were assayed by the PCR-RFLP technique. After amplification of the coagulase gene with specific primers, enzyme restriction was performed using the TaqI enzyme. The amplification of aroA produced an 1153 bp band. Digestion of this band with TaqI enzyme showed 3 different cutting patterns. The first pattern consisted of three bands, the second pattern had two bands, and the third pattern consisted of four bands. 29 S. aureus isolates (out of 40) held the highest number containing the aroA gene with the three-band pattern, 9 samples with the two-band pattern, and 2 samples with the 4-band pattern. Since the method is unable to distinguish between strains with different food origins, it should be replaced by complementary methods. The results indicated the need to pay more attention to health points in the preparation of dairy foods and also more attention of local veterinarians in dealing with this pathogen and choosing the appropriate treatment approach for it.
References:
Buzzola F.R, Barbagelata M.S, Caccuri R.L and Sordelli D. (2006). Attenuation and persistence of and ability to induce protective immunity to a Staphylococcus aureus aroA mutant in mice. Infection and Immunity, 74: 3498–3506.
El-Huneidi, W., Bdour, S. and Mahasneh, A. (2006). Detection of enterotoxin genes seg, seh, sei, and sej and of a novel aroA genotype in Jordanian clinical isolates of Staphylococcus aureus. Diagnostic Microbiology and Infectious disease, 56: 127–132.
Galal, H.M., Abdrabou, M.I., Faraag, A.H., Mah, C.K. and Tawfek, A.M. (2021). Evaluation of commercially available aroA delated gene coli O78 vaccine in commercial broiler chickens under Middle East simulating field conditions. Scientific Reports, 11(1): 1–12.
Grossmann, A., Frobose, N.J., Mellmann, A., Alabi, A.S., Schaumburg, F. and Niemann, S. (2021). An in vitro study on Staphylococcus schweitzeri Scientific Reports, 11(1): 1–10.
Marcos, J.Y., Soriano, A.C., Salazar, M.S., Moral, C.H., Ramos, S.S., Smeltzer, M.S. et al. (1999). Rapid identification and typing of Staphylococcus aureus by PCR-restriction fragment length polymorphism analysis of the aroA Journal of Clinical Microbiology, 37: 570–574.
de Oliveira, M.D., Araújo, J.D.O., Galúcio, J.M., Santana, K. and Lima, A.H., (2020). Targeting shikimate pathway: In silico analysis of phosphoenolpyruvate derivatives as inhibitors of EPSP synthase and DAHP synthase. Journal of Molecular Graphics and Modelling, 101: 107735.
Saei, H.D., Ahmadi, M., Mardani, K. and Batavani, R.A. (2010). Genotyping of Staphylococcus aureus isolated from bovine mastitis based on PCR-RFLP analysis of the aroA Comparative Clinical Pathology, 19(2): 163–168.
Shayegh J., Barzegari A. and Mikaili P. (2013). Molecular characteristics of Staphylococcus aureus isolates from buffalo’s milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19 (4): 665–668.
Seiberling, K.A., Aruni, W., Kim, S., Scapa, V.I., Fletcher, H. and Church, C.A. (2013). The effect of intraoperative mupirocin irrigation on Staphylococcus aureus within the maxillary sinus. International Forum of Allergy & Rhinology, 3(2): 94–98.
Smith, E.M., Green, L.E., Medley, G.F., Bird, H.E., Fox, L.K., Schukken, Y.H. et al. (2005) Multilocus sequence typing of intercontinental bovine Staphylococcus aureus Journal of Clinical Microbiology, 43: 4737–4743.
Stepan, J. Pantucek, R. and Doskar, J. (2004). Molecular diagnostics of clinically important staphylococci. Folia Microbiologica, 49(4): 353–386.
Sung, J.M.L., Lloyd, D.H. and Lindsay, J.A. (2008). Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology, 154(7): 1949–1959.
Zeinhom, M. and Abed, A.H. (2021). Prevalence, characterization, and control of Staphylococcus aureus isolated from raw milk and Egyptian soft cheese. Journal of Veterinary Medical Research, 27(2): 152–160.
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Buzzola F.R, Barbagelata M.S, Caccuri R.L and Sordelli D. (2006). Attenuation and persistence of and ability to induce protective immunity to a Staphylococcus aureus aroA mutant in mice. Infection and Immunity, 74: 3498–3506.
El-Huneidi, W., Bdour, S. and Mahasneh, A. (2006). Detection of enterotoxin genes seg, seh, sei, and sej and of a novel aroA genotype in Jordanian clinical isolates of Staphylococcus aureus. Diagnostic Microbiology and Infectious disease, 56: 127–132.
Galal, H.M., Abdrabou, M.I., Faraag, A.H., Mah, C.K. and Tawfek, A.M. (2021). Evaluation of commercially available aroA delated gene coli O78 vaccine in commercial broiler chickens under Middle East simulating field conditions. Scientific Reports, 11(1): 1–12.
Grossmann, A., Frobose, N.J., Mellmann, A., Alabi, A.S., Schaumburg, F. and Niemann, S. (2021). An in vitro study on Staphylococcus schweitzeri Scientific Reports, 11(1): 1–10.
Marcos, J.Y., Soriano, A.C., Salazar, M.S., Moral, C.H., Ramos, S.S., Smeltzer, M.S. et al. (1999). Rapid identification and typing of Staphylococcus aureus by PCR-restriction fragment length polymorphism analysis of the aroA Journal of Clinical Microbiology, 37: 570–574.
de Oliveira, M.D., Araújo, J.D.O., Galúcio, J.M., Santana, K. and Lima, A.H., (2020). Targeting shikimate pathway: In silico analysis of phosphoenolpyruvate derivatives as inhibitors of EPSP synthase and DAHP synthase. Journal of Molecular Graphics and Modelling, 101: 107735.
Saei, H.D., Ahmadi, M., Mardani, K. and Batavani, R.A. (2010). Genotyping of Staphylococcus aureus isolated from bovine mastitis based on PCR-RFLP analysis of the aroA Comparative Clinical Pathology, 19(2): 163–168.
Shayegh J., Barzegari A. and Mikaili P. (2013). Molecular characteristics of Staphylococcus aureus isolates from buffalo’s milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19 (4): 665–668.
Seiberling, K.A., Aruni, W., Kim, S., Scapa, V.I., Fletcher, H. and Church, C.A. (2013). The effect of intraoperative mupirocin irrigation on Staphylococcus aureus within the maxillary sinus. International Forum of Allergy & Rhinology, 3(2): 94–98.
Smith, E.M., Green, L.E., Medley, G.F., Bird, H.E., Fox, L.K., Schukken, Y.H. et al. (2005) Multilocus sequence typing of intercontinental bovine Staphylococcus aureus Journal of Clinical Microbiology, 43: 4737–4743.
Stepan, J. Pantucek, R. and Doskar, J. (2004). Molecular diagnostics of clinically important staphylococci. Folia Microbiologica, 49(4): 353–386.
Sung, J.M.L., Lloyd, D.H. and Lindsay, J.A. (2008). Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology, 154(7): 1949–1959.
Zeinhom, M. and Abed, A.H. (2021). Prevalence, characterization, and control of Staphylococcus aureus isolated from raw milk and Egyptian soft cheese. Journal of Veterinary Medical Research, 27(2): 152–160.
