The effect of some amino compounds on the structure and activity of RNase A

Subject Areas : environmental management

Farshid Kafilzade ¹ , Sahar Janfeshan ² , Hamid Mohammadi ³

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Received: 2009-05-15 Accepted : 2009-09-16 Published : 2011-06-22

Keywords: RNase A, Amino compound, Putrescine, Cancer,

Abstract :

Increase of bio-environmental pollution due to presence of different kinds of choler and brome compound in Atmosphere, the industrial composition of chemical carcinogens and many other pollutants directly or through the destruction of ozone layer can have cancer effects. Cancer is associated with important inter cell changes such as increasing some of natural amines. Therefore in this research the effect of density increase of some natural amines on structure and function of one of the most important mammals enzyme called rib nuclease A has been studied. First the mentioned enzyme activity has been measured in the both in presence and absence of natural amine compounds       (Putrescine, Spermine, Spermidine, Cadaverine) by the use of spectrophotometer method and in the 284 nm wave length. The results have shown that only putrescence compound caused the decrease of enzyme activity and thus the effect of putrescence on rib nuclease A enzyme structure has been studied by Circular Dhchorism technic .The total results show that putrescene agent causes both the lowering of rib nuclease A activity and  also the disorganization of its second structure.