Antibiotic Resistance in Isolated Negative Gram Bacteria From Intestinal Organ of Anzali Wetland Wild Common Carp

Subject Areas : environmental management

Shila Safaeian ¹ (Assistant Professor, Faculty of Marin Biology (head of the group), Islamic Azad University, Tehran)
Zahra Moghaddam ² (M. Sc. Student, Faculty of Marin Biology, Islamic Azad University, Tehran)
Hedayat Hosseiny ³ (Assistant Professor, Member of the Science Board, Ministry of Health, Research Center, Institute of Research of Nutrition and Food Industry of the Country, Shahid Beheshti University)
Akbar Esmaili ⁴ (Assistant Professor, Faculty of Marin Biology (head of the group), Islamic Azad University, Tehran)

Received: 2009-11-19 Accepted : 2009-06-08 Published : 2014-02-20

Keywords: Negative Gram Bacteria, Antibiotic resistance, Wild Common Carp, Anzali Wetland,

Abstract :

Introduction: A research has been performed on antibiotics' resistance in negative gram bacteriaseparated of wild common carps intestinal organ.Material and Method: 126 negative Gram bacteria have been separated in total and resistancerate of bacteria in relation to 6 Antibiotics are measured: Ampicillin (10 microgram Am),Streptomycin (30 microgram S), Tetracycline (30 microgram T), Chloramphenicol (30 microgram C),Gentamycine (10 microgram GM), Amikacin (30 microgram AK).Results and Discussion: The results showed that negative Gram bacteria in comparison toantibiotic such as Ampicillin (93.5%), Streptomycin (51.15%), Tetracycline (46.5%) had higherresistance. So it showed that such bacteria’s resistance in comparison to Chloramphenicol (12.15%). isrelatively low and it showed loss of existence of resistance in negative Gram bacteria in comparison toGentamycine and Amikacin (0%).Surveying of the resistance rate of bacteria to some antibiotics show that 80% of separated bacteriafrom fish intestinal organ in comparison to consumption antibiotics are multi-resistance.Research shows that antibiotics consumption in animal is growing and more consumption ofantibiotics in environment could cause bioenvironmental problems such as resistance againstantibiotics. This research which was done for the first time in Anzali Wetland on Antibiotic resistancerate in negative Gram bacteria separated of wild common carp digestive system shows that negativegram system resistance against antibiotics in Anzali Wetland is high.

References:

1. Matyar, F., Din, cers, s., kaya, A. &, ColAK, o. (2004). Prevalence and resistance to antibiotics in Gram negative bacteria isolated from retial fish in Turkey. Annals of microbioligy, 54: 151-160.


2. Miranda, C.D. & Zemelman, R. (2001). Antibiotic resistance in fish from the Conception Bay Chile Mar ine Polluttiom Bulletin, 42: 1096–1102.


3. Daschner, F.D. (1980) .In A, von Graevenitz and S. J. Rubin (eds.) .The Genus Serratia. CRC press, Inc., Boca Raton, Fla., pp: 187-196


4. Stewart, K.R., Koditschek, L. (1980). Drug resistance transfer in Escherichia coli in NewYork Bight. Marine Pollution Bulletin, 5: 71-74.


5. Okami, Y., Okazaki, T (1972). Studies on Marine Microorganisms. Isolation from the Japan Sea, the Journal of Antibiotics, pp: 456- 460.


6. Bauer, AW., Kirby,WM ., Sherris, JC., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. Apr; 45(4):493–496.


7. NCCLS. Performance standards for antimicrobial disk susceptibility tests - sixth edition; Approved standard. (1997).NCCLS document M2-A6 (ISBN 1-56238-306-6). NCCLS, 940 West Valley Rd., Suite 1400, Wayne, PA 19087-1898.


8. NCCLS (National Committee for Clinical Laboratory Standards) (2002). Performance Standards for Antimicrobial Susceptibility Testing; Twelfth Informational Supplement. NCCLS document M100-S12, Wayne, Pennsylvania, USA.


9. Hansen, P. K., Lunestad, B.T. and Samuelsen, O.B. (1992). Ecological effects of antibiotics and chemotherapeutants from fish farming, In Chemotherapy in Aquaculture. From Teory to Reality, eds.c. Michel and D. Alderman, pp: 174-178. Office International des Epizooties, Paris.


10. Alves de Lima e Silva, A. and Hofer, E. (1993). Resistance to antibiotics and heavy metals in   Escherichia coli from marine fish. Environmental Toxicology and Water Quality. An International Journal 8: 1-11.


11. Monahan, Robert, L. An Overview of Salmon Aquaculture, Salmon Aquaculture. (1993). Edited by Heen, Knut; Monahan, Robert, L., Utter, Fred. Halsted press, New York.


12. Pathak, S.P. & Gopal, K. (2005). Occurrence of antibiotic and metal resistance in bacteria from organs of river fish. Environmental Research, 98:100-103.


13. Ogbondeminu and F.S and Olayemi. (1993). Antibiotic Resistance in Enteric Bacterial Isolates from fish and water Media. Journal of Aqua. Trop. Vol. 8:pp. 207-212.


14. Chelossi, E., Vezzulli, L., Milano, A., Branzoni, M., Fabiano, M., Riccardi, G., Banat, I. M. (2003).  Antibiotic resistance of benthic bacteria in fishfarm and control sediments of the Western Mediterranean. Aquaculture, 219:83 -97.


15. Honsen, G. H., Olafsen, J. A. (1999). Bacterial interactions in early life stages of marine cold water fish. Microbial Ecology, 38: 1-26.