The effect of mycorrhizal biofertilizer on growth and some glycosides of stevia plant (Stevia rebaudiana Bertoni) in hydroponic cultivation
Subject Areas : Agriculture and Environmentnasrin sadat seyedmohammadi 1 * , morteza barmaki 2 , mahdi davari 3 , kazem hashemi majd 4
1 - PhD Student in Crop Ecology, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Associate Professor, Department of Agriculture and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran
3 - Associate Professor, Department of Plant Medicine, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Associate Professor, Department of Soil Science, University of Mohaghegh Ardabili, Ardabil, Iran
Keywords: Stevia, mycorrhiza, organic, stevioside, rebaudioside.,
Abstract :
Background and Objective: The use of chemicals in agriculture, such as chemical fertilizers, herbicides, and insecticides, will ultimately lead to water and soil poisoning and is a serious threat to the environment. The aim of this study was to use natural growing media and mycorrhizal bio fertilizer, which can play an important role in reducing water and soil pollution and at the same time increase the percentage of stevia glycosides.
Methods: In order to investigate the effect of mycorrhizal fungi on the amount of some stevia glycosides in soilless cultivation, an experiment was conducted in a factorial design based on randomized complete blocks with 4 replications in the 2015-2016 crop year at Mohaghegh Ardabil University. The first factor included growing media including Biolan, vermicomposting, and leaf soil at three levels, and the second factor was inoculation with mycorrhizal fungi and control treatment at two Levels. Keywords: Medicinal herb, Mycorrhiza, Organic, Sativoside, Rebadioside
Results: The results showed that the highest percentage of sativoside with 12.576% was related to the vermicomposting cultivation medium under mycorrhiza inoculation conditions. Also, the highest percentage of rebaudioside A (7.303%) and rebaudioside C (5.388%) was observed in the leaf soil cultivation medium under mycorrhiza inoculation conditions. Leaf fresh weight and leaf dry weight under different cultivation medium conditions and the presence and absence of mycorrhiza fungi were significant at the one percent level, with the highest leaf fresh weight (147.65 g per plant) and the highest leaf dry weight (45.2 g per plant) being obtained in the vermicomposting cultivation medium and under mycorrhiza inoculation conditions.
Conclusion: These treatments can meet the nutritional needs of the plant to a large extent without the slightest damage and environmental hazards and while maintaining the sustainability and health of the agricultural system and the environment. Especially in the case of medicinal plants, including stevia, it leads to the production of healthier active compounds and can also be economically justified in terms of the product market.
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