Effect of salicylic acid on in vitro microtuberization of potato (Solanum microtuberosum)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Received: 2014-10-17 Accepted : 2015-02-09 Published : 2015-09-11

Keywords: Salicylic acid, sante, Microtuberization, MS,

Abstract :

In vitro production of infection- free proliferation for increasing of production is one of the most important objectives in potato seed production. In current research, with the aim of micromicrotuber production, the effect of jasmonic acid and salicylic acid on micromicrotuber production of cultivars Santé we investigated. Single nodes of Sante were cultured on MS medium containing salicylic acid at 0, 2.5, 5, 10 and 20 micromolar and jasmonic acid at 0, 4, 8, 16 and 32 micromolar on micromicrotuberization of Sante was investigated. The experiment was designed based on complete randomized design. The results indicated that application of jasmonic acid induced micromicrotuberizatin in comparison to the control. But jasmonic acid has no effect on micromicrotuber size and wt. Salicylic acid at 2.5, 5 and 10 micromolar in comparison to 20 micromolar and control showed higher number of micromicrotubers. Salicylic acid has no effect on micromicrotuber wt. Furtheremore, it seems that the high concentration of salicylic acid and jasmonic acid inhibit microtuberization. ze:11.0pt;mso-bidi-font-family: "B Nazanin";mso-bidi-language:FA'>MS)حاویتیمارسالیسیلیکاسیددرغلظت‌های0،5/2،5،10و20میکرومولاروجاسمونیکاسیددرغلظت‌های0،4،16و32میکرومولارکشتشدند.اینآزمایشبهصورتفاکتوریلدرقالبطرحکاملاًتصادفیانجامشد.دراینآزمایشصفاتتعدادبرگ،طولساقه،تعدادریزغدهووزنریزغده­هایتولیدیارزیابیشدند.نتایجنشاندادندکهکاربردجاسمونیکاسیدنسبتبهتیمارشاهدباعثافزایشتولیدریزغدهگردید.امابروزنریزغده­هایتولیدیمؤثرنبود.همچنینغلظت‌هایبالایجاسمونیکاسیدسببکاهشتعدادریزغده‌هاگردیدواثربازدارندهداشت.غلظت­های5/2،5و10میکرومولارسالیسیلیکاسیدنسبتبهتیمار20میکرومولاروشاهدباعثافزایشتولیدریزغدهگردید.اماتأثیرسالیسیلیکاسیدبروزنریزغده­هامعنی­دارنبود.  

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