A study on the developmental stages of the embryo sac in Amygdalus scoparia L.

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Received: 2014-12-11 Accepted : 2015-01-23 Published : 2015-06-10

Keywords: Morphology, Rosaceae, emryo,

Abstract :

Amygdalus scoparia L. is an important species of the family Rosaceae with medicinal and antioxidant properties. Amygdalus is morphologically highly diverse such that their identification requires more than morphological characteristics. To reveal the stages of Amygdalus scoparia L embryogenesis, the different developmental stages of the generative organs was subjected to both cytological and histological examination. For both cytological and histological purposes, samples were first fixed, and sections were prepared using a microtome following rinsing, dehydration and saturation with paraffin. Sections were then stained by Hemotoxin-eosin and photographed under a camera equipped light microscope. Samples prepared from different developmental stages of the flowers revealed a complete form of the flowers with peripheral gynoecium Corolla was continuous consisting of 5 sepals, 5 petals and 10-40 stamens, while gynoecium with a superior ovary was of single chamber with a single carpel containing two anatrope eggs. Of which one is usually fertilized. Stages of the embryogenesis and the embryo sac development included: spherical, heart-shaped, torpedo, cotyledonous. It appears that embryogenesis and the embryo sac development in Amygdalus scoparia L. has a similar pattern as that of dicots and other members of the Rosaceae family.

References:

 

[1]     تهرانی‌فر،ع، کافی، م، عدلی، م، 1377، پرورشبادام، انتشاراتدانشگاهیمشهد.

[2]     خاتم‌ساز، محبوبه،1371، تیرهگل‌سرخRosaceae،فلورایران، شماره6، موسسهتحقیقاتجنگل‌هاومراتع.

[3]     طلایی، ایمانی، ع، 1376، معرفیدورقم بادامجدیددیرگلوپرباروبررسیخصوصیاتمورفولوژیوپومولوژیآنها، رسالهدورهدکترا.

[4]    Dumas, C., Mogensen, H.L., 1993, Gametes and fertilization: maize as a model system for experimental embryogenesis in flowering plants, Plant Cell. 5: 1337-48.

[5]    Egea, J., Burgos, L., 2000, Ovule differences between singlekernelled and double-kernelled fruits in almond (Prunusdulcis). Ann. Appl. Biol. 136: 291–295.

[6]    Goodner, B., Quatrano, R.S., 1993, Fucus embryogenesis: a model to study the establishment of polarity, Plant Cell. 5: 1471-81.

[7]    Hedge, I. C., Amygdalus L., 1968, (In Flora Iranica, K.H. Rechinger, ed.), AkademischeDruck-u.Verlagsandstalt, Graz) 69:160-184.

[8]    Imain, A., 1997, Effect of biological and physiological characteristics on the selected almond varieties these PhD TarbiatModdares University of Tehran Iran, 256.

[9]    IrannejadParizi, M., 1374, Ecologic survey of Amygdalusschoparia natural types societies in Kerman province, forestry Msc thesis, natural resources college, Tarbiatmodarres university, 145.

[10]Jakovlev, M.A., 1985, Sraviteľnajaembryologijacvetkovychrastenij, Nauka, Leningrad. 133.

[11]Lersten, N., 2004, Flowering plant Embryology, Blackwell publishing.

[12]Mikatadze-Pantsulaya, T. A., 1985, A study of the development of the female generative sphere of some species of Amygdalus, L. SoobscheniyaAkademiiNaukGruzinskoi, SSR 117: 577–58.

[13]Oukabli, A., Lansari, A., Wallali, D. L., Abousalim, A., Egea, J., Michaux-Ferriere, N., 2002, Self and cross pollination effects on pollen tube growth and fertilization in self-compatible almond Prunusdulciscv, “Tuono”. J. Hortic. Sci. Biotech. 75: 739–744.

[14]Pimienta, E., Polito, V. S., 1983, Embryo sac development in almond [Prunusdulcis (Mill.), D.A.Webb] as affected by cross-, self- and non-pollination, Ann. Bot. 51: 469–479.

[15]Souter, M., Lindsey, K., 2000, Polarity and signaling in plant embryogenesis, Journal of experimental Botany, 347, 971-983.