Study of the repair of bone defect using chitosan - gelatin membrane and adipose - derived stem cells in Albino Wistar adult rat (histomorphometrical and immunological evaluations)
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Keywords: Rat, ADSCs, Chitosan–Gelatin membrane, Cell transplantation, Bone Repair,
Abstract :
Adipose - derived stem cells are multipotent cells that capable to differentiate into osteogenic cells, the scaffold of chitosan and gelatin as biodegradable and compatible used for tissues repair. The control group that received no treatment after the bone defect, 2 sham : that the defect in the bone defect was injected medium, 3- (gelatin - chitosan) that the membrane gelatin - chitosan was used into the bone defect. 4. ADSC: that ADSC cell transplanted into the defect. 5 : (cell - the cell membrane of gelatin – chitosan) was used into the bone defect. Histomorphometric evaluations in the different groups studied. The mean area of trabeculae in groups of membranes, cells and cell - membrane significantly increased when compared to the control group. Also the mean number of osteocytes and cells in the bone defect in groups of cells and cell - membrane significantly decreased when compared to the control group. Although in the chitosan - gelatin group, mean number of osteocytes decreased but were not significant. It seems that use of scaffold of chitosan - gelatin membrane and ADSC cell transplantation to be effective in repair of bone defect.
[1] Abukawa H, Shin M, Williams WB, Vacanti JP, Kaban LB, Troulis MJ. 2004.Reconstruction of mandibular defects with autologous tissue-engineered bone. J Oral MaxillofacSurg. ;62:601–6. [PubMed]
[2] Arthur, A. Zannettino, A AND Gronthos, S. 2009 .The Therapeutic Applications of MultipotentialMesenchymalStromal Stem Cells in Skeletal Tissue Repair. J. Cell. Physiol., 218: 237–245.
[3] Ashton BA, Allen RD, Howlett CR, Eaglesom CC, Hatton A, Owen M. 1980 .Formation of bone andcartilage by marrow stromal cells in diffusion chambers in vivo. ClinOrthopRelatRes;(151):294-307.
[4] B. Niechoda,Y. YuandW.R. Walsh .2000. Healing bon with adipose tissue derived stromal stem cells – the histomorfhometry of the ovin defect model. Orthopaedic Research Laboratories, Prince of Wales Hospital, University of New South Wales, Sydney, Australia:
[5] Brownlow HC, Simpson AH. 2000.Metabolic activity of a new atrophic nonunion model in rabbits. J OrthopRes May; 18(3):438-42.
[6] Bruder SP, Kraus KH, Goldberg VM, Kadiyala S. 1998.The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects. J Bone Joint Surg Am; 80:985–996.
[7] Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C.2008. Adipose-derived stem cells: Isolation, expansion and differentiation.Methods;45:115–20.
[8] Cao L, Liu, G. Gan, Y. Zhang, X. Tang, T. Dai, K. et al. (2012). The use of autologous enriched bone marrow MSCs to enhance osteoporoticbone defect repair in long-term estrogen deficient goats. Biomaterials 33و 5076e5084.
[9] Cheryl, T., Gomillion, Karen J.L. 2006, Stem cells and adipose tissue engineering. Biomaterials;.27:6052–6063.
[10]Choi JH, Gimble JM, Lee K, Marra KG, Rubin JP, Yoo JJ, et al .2010.Adipose tissue engineering for soft tissue regeneration. Tissue EngPart B Rev;16(4):413-26.
[11]Crha, M. Nečas, A. Srnec, R. Janovec, J. Stehlík, L. Raušer, P. et al. (2009). Mesenchymal Stem Cells in Bone Tissue Regeneration and Application to Bone Healing. ACTA VET. BRNO, 78: 635-642.
[12]De Ugarte DA, Morizono K, Elbarbary A, Alfonso Z, Zuk PA, Zhu M, et al. 2003.Comparison of multi-lineage cells fromhuman adipose tissue and bone marrow. Cells Tissues Organs.; 174(3): 101-9.
[13]Dupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE. 2010. Human stem cell delivery for treatment of large segmental bone defects. Proc Natl AcadSci U S A. Feb 23;107(8):3305-10.
[14]Elahi M, Kabirsoleimani M, Shiravi A. 2011. Investigate the possibility ofusing enzymes to extract mesenchymal stem cells from human adipose tissue. J Med Res;4(35):200-80. [Persian]
[15]Fan J, Varshney RR, Ren L, Cai D, Wang DA. 2009. Synovium-derived mesenchymal stem cells: A new cell source for musculoskeletal regeneration. Tissue Eng Part B Rev.;15:75–86. [PubMed]
[16]Gimble JM, Katz AJ, Bunnell BA. 2007. Adipose-derived stem cells for regenerative medicine. Circ Res; 100(9):
[17]Goldberg V.M. and Caplan A.I., 2004.Orthopedic Tissue Engineering, Marcel Dekker, New York, Chap. 1.
[18]Habraken, W., J. G. C. Wolke, and J. A. Jansen, 2007. Ceramic composites as matrices and scaffolds for drugdelivery in tissue engineering. Advanced drug delivery reviews,. 59(4): p. 234-248.
[19]Haghighat A, Hekmatian E, Abdinian M, Sadeghkhani E.2011. Radiographic Evaluation of Bone Formation and Density Changes after Mandibular Third Molar Extraction: A 6 Month Follow up.Dent Res J; 8:1–5. [PMC free article][PubMed]
[20]Hollister, S. J. 2005. Porous scaffold design for tissue engineering. Nature materials, 4(7): p. 518-524.
[21]Hong SR, Lee SJ, Shim JW, Choi YS, Lee YM, Song KW, et al. 2001. Study on gelatin-containingartificial skin IV: A comparative study on theeffect of antibiotic and EGF on cell proliferationduring epidermal healing. Biomaterials; 22(20): 2777-83
[22]Huang Y, Onyeri S, Siewe M, Moshfeghian A,Madihally SV. 2005. In vitro characterization ofchitosan-gelatin scaffolds for tissue engineering.Biomaterials; 26(36): 7616-27.
[23]Kim KM, Evans GRD. 2005. Tissue Engineering: The Future of Stem Cells. In: Ashammakhi N, Reis RL, editors. Topics in tissue engineering.
[24]Kyunghee Lee, Hyunsoo Kim, Jin-Man Kim, Jae-Ryong Kim, Keuk-Jun Kim,Yong-Jin, Se-Il Park, Jae-Ho Jeong, Young-mi Moon, Hyun-Sook Lim, Dong-Won Bae, Joseph Kwon, Chang-Yong Ko, Han-Sung Kim, Hong-In Shin, DaewonJeong.(2011), Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblas and osteoclast function,Journal of Cellular and Molecular Medicine,J. Cell. Mol. Med. Vol 15, No 10, 2011
[25]Liao D, Gong P, Li X, Tan Z, Yuan Q. 2010. Co-culture with Schwann cells is an effective way for adipose-derived stemcells neural transdifferentiation. Arch Med Sci.; 6(2): 145-51.
[26]Liu B1,Cui L,Liu GP,Cao YL,Zhu JT,Cao Y. )2009(,Tissue-engineering bone with ADSCs and coral scaffold for repairing of cranial bone defect in canine.Zhonghua Zheng Xing Wai KeZaZhi.2009. May;25(3):204-8.
[27]Nather A, David V, Teng JW, Lee CW, Pereira BP. 2010. Effect of autologous mesenchymal stem cells on biological healing of allografts in critical-sized tibial defects simulated in adult rabbits. Ann Acad Med Singapore. Aug;39(8):599-606.
[28]Nichol JW, Khademhosseini A. 2009. Modular Tissue Engineering: EngineeringBiological Tissues from the Bottom Up. Soft Matter;5(7):13129.
[29]Olszta, M. J., Olszta, M. J., Cheng, X., Jee, S. S., Kumar, R., Kim, Y. Y., Kaufman, M. J., Douglas,. P., Gower, L. B., 2007. Bone structure and formation: A new perspective. Materials Scienceand Engineering: R: Reports,. 58(3): p. 77-116.
[30]Schliephake H, Zghoul N, Jager V, van Griensven M, Zeichen J, Gelinsky M, et al. 2009. Bone formation in trabecular bone cell seeded scaffolds used for reconstruction of the rat mandible.Int J Oral Maxillofac Surg.;38:166–72.[PubMed]
[31]Stockmann, Ph. Park, J. Wilmowsky, v. Nkenke, C. Felszeghy, E. Friedrich, J. et al. 2012 .Guided bone regeneration in pig calvarial bone defects using autologous mesenchymal stem/progenitor cells e A comparison of different tissue sources. Journal of Cranio-Maxillo-Facial Surgery, 40; 310e320.
[32]Wilson, ADH., Hart, A., Brannstrom ,T., Wiberg ,M., Terenghi, G., 2003,Primary sensory neuronal rescue with systemicacetyl-L-carnitine following peripheral axotomy, A dose-response analysis, British Journal of PlasticSurgery, 56:732-739.
[33]Yoshimura H, Muneta T, Nimura A, Yokoyama A, Koga H, Sekiya I. 2007;Comparison of rat mesenchymal stem cellsderived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res. 327(3): 449-62.