A Comparison of Chicken-Derived Fibroblast Cells and Quail Cells Based on Proliferation and Viability Potential

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

elham hoveizi ¹ (Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran)
Ali Aghaei ² (Department of Animal Science, Faculty of Animal and Food Sciences, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran.)

Received: 2022-06-08 Accepted : 2023-03-10 Published : 2023-07-23

Keywords: quail, growth rate, cell viability, Avian stem cells,

Abstract :

The avian stem cells are an excellent in vitro model for development and pharmacology educations. These cells have the potential for self-renewal and differentiation and can be considered as a valuable technology in the poultry industry. The purpose of this study was the derivation, culture, and comparison of chicken fibroblast cells to quail cells, regarding cell doubling time and the rate of cell viability during various cell passages. In this experimental study, fertilized eggs were obtained and fibroblasts were isolated with an enzymatic digestion method and of cultured in DMEM medium including 10% FBS for various passages. Population doubling time (PDT) and cell viability were evaluated by trypan blue staining and MTT assay. Data indicated that both cells had a maximum proliferation when cultured in a DMEM containing 10% FBS at 38 C˚. Based on our results, PDT was measured 16±2 h for both two cells during the first o third passages. But, the population of the chicken fibroblasts was doubled in number each 28±2 h, while this value was 36±2.1 h for quail cell population (p<0.05) during the third to sixth passages. Also, cell viability results were according to PDT results. This study demonstrated a potential of self-renewal and cell viability of quail fibroblast cells as a new avian cell source and suggested to use of these cells in future manufacturing applications.

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