مقایسه سلولهای فیبروبلاست مشتق شده از جنین جوجه و بلدرچین بر اساس توانایی تکثیر و بقا
محورهای موضوعی :
زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
الهام حویزی
1
,
علی آقائی
2
1 - گروه زیست شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران
2 - گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران
تاریخ دریافت : 1401/03/18
تاریخ پذیرش : 1401/12/19
تاریخ انتشار : 1402/05/01
کلید واژه:
سرعت رشد,
بلدرچین,
سلولهای بنیادی ماکیان,
بقای سلولی,
چکیده مقاله :
سلولهای بنیادی ماکیان مدلهای درون آزمایشگاهی فوق العاده ای در آموزشهای تکوینی و دارویی محسوب میشوند. این سلولها دارای ظرفیتهای بالایی در خودنوزایی و تمایز بوده و میتوانند به عنوان فناوری ارزشمندی در صنعت طیور مورد توجه قرار گیرند. هدف از این پژوهش استحصال، کشت و مقایسه سلولهای فیبروبلاست جنین جوجه و بلدرچین با درنظر گرفتن زمان دوبرابر شدن تعداد سلولی (PDT) و میزان بقای سلولی در طول دورههای مختلف پاساژ بود. در این پژوهش تجربی، سلولهای فیبروبلاست با روش هضم آنزیمی از تخمهای لقاح یافته جدا و در محیط کشت DMEM محتوی 10درصد سرم FBS برای پاساژهای مختلف کشت شدند. زمان دو برابر شدن تعداد سلولها و بقای سلولی با استفاده از رنگآمیزی تریپان بلو و تستMTT بررسی گردید. دادهها نشان داد که هر دو نوع سلول در محیط کشت DMEM محتوی 10درصد سرم FBS و در دمای 38 درجه دارای حداکثر تکثیر بودند. براساس نتایج ما PDT برای هر دونوع سلول حدود 2±16 ساعت در طول پاساژهای اول تا سوم محاسبه گردید، اما در طی پاساژهای چهارم تا ششم PDT برای سلولهای فیبروبلاست جوجه به 2±28 ساعت و برای سلولهای فیبروبلاست بلدرچین 2±36 ساعت محاسبه گردید (p<0.05)؛ همچنین نتایج تست بقای سلولی مطابق با نتایج PDTبود. این پژوهش توانایی خودنوزایی و بقای سلولهای فیبروبلاست بلدرچین بهعنوان منبعی جدید از سلولهای بنیادی ماکیان را توصیف میکند و استفاده ازین سلولها در کاربردهای فناوردی در آینده را پیشنهاد میکند.
چکیده انگلیسی:
The avian stem cells are an excellent in vitro model for development and pharmacology educations. These cells have the potential for self-renewal and differentiation and can be considered as a valuable technology in the poultry industry. The purpose of this study was the derivation, culture, and comparison of chicken fibroblast cells to quail cells, regarding cell doubling time and the rate of cell viability during various cell passages. In this experimental study, fertilized eggs were obtained and fibroblasts were isolated with an enzymatic digestion method and of cultured in DMEM medium including 10% FBS for various passages. Population doubling time (PDT) and cell viability were evaluated by trypan blue staining and MTT assay. Data indicated that both cells had a maximum proliferation when cultured in a DMEM containing 10% FBS at 38 C˚. Based on our results, PDT was measured 16±2 h for both two cells during the first o third passages. But, the population of the chicken fibroblasts was doubled in number each 28±2 h, while this value was 36±2.1 h for quail cell population (p<0.05) during the third to sixth passages. Also, cell viability results were according to PDT results. This study demonstrated a potential of self-renewal and cell viability of quail fibroblast cells as a new avian cell source and suggested to use of these cells in future manufacturing applications.
منابع و مأخذ:
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Davey MG, Balic A, Rainger J, Sang HM, McGrew MJ. Illuminating the chicken model through genetic modification. Int J Dev Biol. 2018; 62(1-2-3): 257-64.
Shittu I, Zhu Z, Lu Y, Hutcheson JM, Stice SL, West FD, et al. Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine. Biologicals. 2016; 44(1): 24-32.
Li Y, Ming F, Huang H, Guo K, Chen H, Jin M, et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Scientific reports. 2017; 7:40698.
Vargas-Sanchez RD, Ibarra-Arias FJ, Torres-Martinez BDM, Sanchez-Escalante A, Torrescano-Urrutia GR. Use of natural ingredients in the Japanese quail diet and their effect on carcass and meat quality. Review. Asian-Australas J Anim Sci. 2019: 1641-56.
Pramod RK, Lee BR, Kim YM, Lee HJ, Park YH, Ono T, et al. Isolation, Characterization, and In Vitro Culturing of Spermatogonial Stem Cells in Japanese Quail (Coturnix japonica). Stem cells and development. 2017;26(1):60-70.
Sobhani A, Khanlarkhani N, Baazm M, Mohammadzadeh F, Najafi A, Mehdinejadiani S, et al. Multipotent Stem Cell and Current Application. Acta Med Iran. 2017; 55(1): 6-23.
Wood SM, Wood JN. A chicken model for studying the emergence of invariant object recognition. Front Neural Circuits. 2015; 9:7.
Zheng D, Wang X, Xu RH. Concise Review: One Stone for Multiple Birds: Generating Universally Compatible Human Embryonic Stem Cells. Stem Cells. 2016; 34(9): 2269-75.
Farzaneh M, Attari F, Khoshnam SE, Mozdziak PE. The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system. Br Poult Sci. 2018; 59(2): 240-4.
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Tawk M, Vriz S. [Regeneration of vertebrate appendage: an old experimental model to study stem cells in the adult]. Med Sci (Paris). 2003; 19(4): 465-71.
Hoveizi E, Ebrahimi‐Barough S. Embryonic stem cells differentiated into neuron‐like cells using SB431542 small molecule on nanofibrous PLA/CS/Wax scaffold. Journal of cellular physiology. 2019; 234(11): 1956-1973.
Tolik D, Polawska E, Charuta A, Nowaczewski S, Cooper R. Characteristics of egg parts, chemical
composition and nutritive value of Japanese quail eggs--a review. Folia Biol (Krakow). 2014; 62(4): 287-92.
Intarapat S, Stern CD. Chick stem cells: current progress and future prospects. Stem Cell Res. 2013;11(3): 1378-92.
Farzaneh M, Hassani SN, Mozdziak P, Baharvand H. Avian embryos and related cell lines: A convenient platform for recombinant proteins and vaccine production. Biotechnology journal. 2017; 12(5).
Abdolmaleki A, Zahri S. Comparison of toxicity and teratogenic effects of salen and vo-salen on chicken embryo. Drug Chem Toxicol. 2016; 39(3): 344-9. Epub 2015/12/25.
Morin V, Veron N, Marcelle C. CRISPR/Cas9 in the Chicken Embryo. Methods Mol Biol. 2017; 1650: 113-23.
Kwon MS, Koo BC, Kim D, Nam YH, Cui XS, Kim NH, et al. Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites. PloS one. 2018; 13(5): e0194721.
Farzaneh M, Attari F, Mozdziak PE, Khoshnam SE. The evolution of chicken stem cell culture methods. Br Poult Sci. 2017; 58(6): 681-6.
Davey MG, Balic A, Rainger J, Sang HM, McGrew MJ. Illuminating the chicken model through genetic modification. Int J Dev Biol. 2018; 62(1-2-3): 257-64.
Shittu I, Zhu Z, Lu Y, Hutcheson JM, Stice SL, West FD, et al. Development, characterization and optimization of a new suspension chicken-induced pluripotent cell line for the production of Newcastle disease vaccine. Biologicals. 2016; 44(1): 24-32.
Li Y, Ming F, Huang H, Guo K, Chen H, Jin M, et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Scientific reports. 2017; 7:40698.
Vargas-Sanchez RD, Ibarra-Arias FJ, Torres-Martinez BDM, Sanchez-Escalante A, Torrescano-Urrutia GR. Use of natural ingredients in the Japanese quail diet and their effect on carcass and meat quality. Review. Asian-Australas J Anim Sci. 2019: 1641-56.
Pramod RK, Lee BR, Kim YM, Lee HJ, Park YH, Ono T, et al. Isolation, Characterization, and In Vitro Culturing of Spermatogonial Stem Cells in Japanese Quail (Coturnix japonica). Stem cells and development. 2017;26(1):60-70.
Sobhani A, Khanlarkhani N, Baazm M, Mohammadzadeh F, Najafi A, Mehdinejadiani S, et al. Multipotent Stem Cell and Current Application. Acta Med Iran. 2017; 55(1): 6-23.
Wood SM, Wood JN. A chicken model for studying the emergence of invariant object recognition. Front Neural Circuits. 2015; 9:7.
Zheng D, Wang X, Xu RH. Concise Review: One Stone for Multiple Birds: Generating Universally Compatible Human Embryonic Stem Cells. Stem Cells. 2016; 34(9): 2269-75.
Farzaneh M, Attari F, Khoshnam SE, Mozdziak PE. The method of chicken whole embryo culture using the eggshell windowing, surrogate eggshell and ex ovo culture system. Br Poult Sci. 2018; 59(2): 240-4.