Differentiation of amniotic membrane mesenchymal stem cells (hAMSCs) cardiomyocyte on PGS-co-PCL / PGC / PPy / Gelatin scaffold and its effect in the treatment of myocardial ischemia in adult male Wistar rats

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

mahsa Ale Ebrahim ¹ , NASTARAN BAHRAMI ² , nooshin barikrow ³ , yasin asadi ⁴ , fateme roholah ⁵ , ali salimi ⁶

1 - Departments of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 - Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
3 - Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
4 - Departments of Physiology, Kish International Branch, Islamic Azad University, Kish Island, Iran.
5 - 1 Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
6 - Nanobiotechnology research center, Baqiyatallah University of medical sciences, Tehran, Iran.

Received: 2022-05-28 Accepted : 2022-08-27 Published : 2023-03-21

Keywords: Mesenchymal stem cells, Amniotic membrane, PGS-co-PCL / PGC / PPy / Gelatin scaffold, Ischemia reperfusion,

Abstract :

AbstractIntroduction: The cardiac ischemia and its subsequent failures have a high prevalence and its treatment is dangerous, expensive and ineffective. With the implant of mesenchymal stem cells, researchers hope to replace the dead tissues and reactivate the damaged parts of the heart. Materials and methods: After the differentiation of mesenchymal stem cells of the amniotic membrane into heart muscle cells, by closing the left anterior descending coronary artery (LAD) for 45 minutes, acute ischemia was induced in male Wistar rats. Then reperfusion was performed in male rats. Then treatment groups, including: differentiated stem cells, stem cells with PGS-co-PCL/PGC/PPy/Gelatin scaffold and scaffold group, were implanted into the damaged heart. After 2 and 4 weeks, echocardiography was performed and angiogenesis was examined in the heart tissue using the immunohistochemical technique.Results and discussion: In treatment groups with mesenchymal cells and scaffolds + mesenchymal cells compared to the control, the amount of cardiac ejection fraction (EF), ejection fraction (FS) and cardiac stroke volume (SV) increases. Also, by examining the growth of VEGF in different groups and the control group, there was a significant increase in the expression of this angiogenic factor in the group receiving the scaffold + mesenchymal cells compared to other groups, which seems to use the PGS-co-PCL/PGC/PPy./Gel scaffold has created enough and suitable space for growth of vessels in the myocardium.