Anti-cancer effect of Artemisia turcomanica extract on breast cancer cell line (MCF-7)
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
zahra
keshtmand
1
(, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.)
Seyede narges
naimi
2
(Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.)
, Ardeshir
Hesampour
3
(Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
Keywords: Apoptosis, Artemisia turcomanica, viability cell, Breast cancer cell line(MCF-7),
Abstract :
The prevalence of cancer and the side effects of treatment methods have provided a growing interest in the use of plants as a promising source of treatment.The use of medicinal plants in traditional medicine has been important for a long time and many of these medicinal plants have antioxidant and anticancer effects. The purpose of this study is to investigate the anticancer effects of Artemisia turcomanica extract on breast cancer cell line (MCF-7)and its effect on Bcl2 and Bax gene expression.In this experimental study, the effect of the hydroalcoholic extract of Artemisia turcomanica on viability and expression of Bax and Bcl2 genes in MCF-7 breast cancer cells was examined.For this purpose,MCF-7breast cancer cells were cultured and treated with the hydroalcoholic extract of Artemisia turcomanica(with different concentrations)for 24,48 and72 hours.The effect of the extract on cell viability was evaluated by MTT method.Next,RNA extraction was performed and after cDNA synthesis,the expression level of Bax and Bcl2 genes was measured by Real-time PCR method.This research showed that with the increase in concentration and time,the viability of the cells decreased significantly compared to the control samples.Also, the results of real-time PCR showed that the expression of Bax and Bcl2 genes changed significantly compared to the control sample at 48 and72 hours.The results showed that the extract of Artemisia turcomanica has cytotoxic and apoptotic effects on the MCF-7 cell line, so that by conducting more studies, the extract of this plant can be used as an anti-cancer biological product in the treatment of cancer
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