Investigation of the frequency of exoA and exoT genes in clinical isolates of Pseudomonas aeruginosa and antibiotic resistance of strains

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Shadi Mahboubi ¹ , Somayeh Ataei-e Jaliseh ²

1 - Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.
2 - 1. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.

Received: 2022-10-04 Accepted : 2023-01-01 Published : 2023-02-20

Keywords: PCR, Pseudomonas aeruginosa, exoA gene, exoT gene,

Abstract :

Pseudomonas aeruginosa is a gram-negative bacillus and an opportunistic pathogen. This bacterium is the third most common cause of nosocomial infections. Exoenzymes T, S, U, Y are secreted by the type III secretion system, which is one of the important pathogenic factors of this bacterium, and exotoxin A is secreted by the type II secretion system. In this study, the frequency of exoA and exoT genes in clinical strains of Pseudomonas aeruginosa and the antibiotic resistance of the strains were investigated. 29 strains of P. aeruginosa bacteria were identified from patients admitted to Razi Hospital in Rasht, using common microbiology methods and were examined along with the standard strain of ATCC27853. The resistance of these strains to 6 types of antibiotics was determined using disk diffusion method. Then DNA extraction and PCR was performed to determine the frequency of exoA and exoT genes. In this research, the highest rate of antibiotic resistance was observed for Imipenem(86.75). The results obtained from PCR showed a frequency of 37.93%for exoA and 68.96% for exoT.