The expression and secretion of Human papilloma L1 heterologous protein of genotype11 in lactobacillus
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Akram Amani
1
,
Parvaneh Jafari
2
,
Rodabeh Behzadi Andohjerdi
3
,
Jalil Fallah
4
1 - Department of Microbial Biotechnology, Central Tehran Branch, Islamic Azad University of Tehran, Iran
2 - Department of Microbiology, Faculty of Basic Sciences, Arak Branch, Islamic Azad University of Arak, Iran
3 - Department of Biology, Central Tehran Branch, Islamic Azad University of Tehran Iran
4 - Department of Microbiology, Lister Microbiology Laboratory, Tehran, Iran
Keywords: cervical cancer, Human Papillomavirus, Lactobacillus bacteria, Heterologous L1 protein, Genital warts,
Abstract :
Background: Human papillomavirus is a virus that transmitted through sexual intercourse and direct contact between human skin. Among the different strains of this virus, strains 18 and 16 are the most common strains in the development of cervical cancer, and also strains 11 and 6 are the most common strains in the development of genital lesions or genital warts. In this study, we used strain 11 capsid for secretory protein production Large L1 in the Lactobacillus host.Materials and Methods: To produce L1 protein from human papillomavirus, recombinant protein production methods were used by cloning the L1 gene of this virus into a vector expressed in a prokaryotic host such as Lactobacillus, which was analyzed by SDS-PAGE methods. SDS-PAGE and Western blotting have been evaluated to confirm the expression of the protein.Results: Nucleotide bands were confirmed in the gene fragment amplification experiment in gel electrophoresis as well as the observation of protein bands in SDS-PAGE and Western blot gels.Conclusion: By using the production of pathogenic protein in human papillomavirus by the recombinant method and in the bacterial host Lactobacillus, which is a fluorescence of the female reproductive system, can be used in the future to prevent and treat diseases associated with strain 11 of this virus.
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