The effect of menthol on learning, spatial memory and GABBR2 gene expression in male epileptic rats with pentylenetetrazole

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Azadeh Karimi Anjiraki ¹ , Ramesh Ahmadi ² , Hamid reza Mohajerani ³

1 - Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
2 - Assistant Professor, Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
3 - Department of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran

Received: 2022-01-14 Accepted : 2022-08-15 Published : 2022-08-23

Keywords: Learning, menthol, epilepsy, GABA, spatial memory,

Abstract :

Background:There is a relationship between epilepsy and decreased daily memory, learning and behavioral disorders, so in this study, the effect of menthol on learning and spatial memory and GABA receptor gene expression in epileptic rats was investigated.Materials and Methods: In this study, 60 male rats were randomly divided into 6 groups of 10, including control , ethanol, pTZ (37.5 mg / kg), menthol (30 mg / kg) and ethanol + PTZ and Menthol + PTZ groups Chemical kindling was performed over a 28-day period with 14 injections of pentylenetetrazole every other day, then spatial learning and memory were tested by the Morris water maze .Then, the animals were anesthetized and their brains were isolated for GABBR2 gene expression. For statistical analysis of data, one-way and two-way ANOVA and Tukey test were used at a significant level of P <0.05. Delta CT test was used to express the gene.Finding: PTZ decreased learning and memory and menthol was able to prevent this defect and increase learning and memory, also menthol was able to increase GABBR2 gene expression in epileptic rats. Conclusion: Pretreatment with menthol significantly reduced the incidence of epilepsy in epileptic rats with pentylenetetrazole. Menthol increased learning, spatial memory, and GABA receptor gene expression in epileptic rats, Therefore, menthol may be a suitable choice for the treatment and reduction of learning and memory disorders caused by epilepsy.