Inhibitory Effects of Small Molecule TPSF on Growth and Proliferative of Human Breast Cancer Cell Line MCF-7: A Laboratory Study

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Kiavash Hoshmndi ¹ , elham hoveizi ² , SAAD GOORANI NEJAD ³ , mohammadreza Tabandeh ⁴

1 - Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 - DEPARTMENT OF CLINICAL SCIENCES, FACULTY OF VETERINARY MEDICINE, SHAHID CHAMRAN UNIVERSITY OF AHVAZ
4 - Associate Prof, Dept of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Received: 2021-07-18 Accepted : 2021-12-29 Published : 2022-02-20

Keywords: breast cancer, MCF-7 Cell Line, Small molecule, TPSF,

Abstract :

Breast cancer among women cancers has been the first to suffer from mortality and morbidity. Today, small molecules are still dominating the field of drug innovation, because of their special properties. The aim of this study is the investigation of the growth inhibitory and anti-proliferation effects of TPSF (4-(4-Fluorophenyl)-4-oxobutyl]thio]-1,3,6,9-tetrahydro-1,3-dimethyl-6-thioxo-2H-purin-2-one) on MCF-7 breast cancer cell line. In this laboratory study, MCF-7 breast cancer cells were cultured in a medium containing serum 10% and the cells were treated with the concentrations of 0.5, 1, 2, 5, and 10 of TPSF in 1, 3, and 5 days. Then the inhibitory effects of TPSF on cell growth and proliferation were assessed by MTT assay. Molecular docking was performed using Molegro software. For data analysis, two-way ANOVA and Tukey's post hoc test were used. According to the results, TPSF significantly (P≤0.001) decreases the survival rate and proliferation of MCF-7 cells in a time and dose-dependent manner. So that, the cell viability of cells treated with concentrations of 0.5, 1, 2, 5, and 10 µM were 90, 79, 68, 50, and 90 % respectively after 24h that confirmed that cell viability significantly decreases (P≤0.001) depended on dose, and the concentration of 5 µM was determined as the IC50 concentration. The study of morphological showed changes consisting of cell shrinkage, nucleotide pigmentation, and fragmentation of chromatin. The docking results also confirmed the interaction of TPSF with HER2 and ER.TPSF inhibits the growth of breast cancer cells and can be regarded as an anticancer medicine.