Comparison of Antifungal Effects of Aquatic and Alcoholic Extract of Mentha pulegium L.With Fluconazole on Growth of Candida Albicans

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Tohid Piri Gharaghie ¹ , Sheida Beiranvand ² , Samea Hajimohammadi ³

1 - Department of Biotechnology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2 - Department of Biotechnology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
3 - Department of Electrical Engineering, Faculty of Engineering, Ivaneki University , Ivaneki, Iran

Received: 2020-10-05 Accepted : 2021-02-07 Published : 2021-05-22

Keywords: Candida albicans, Fluconazole, Mentha pulegium extract, MIC and MFC tests,

Abstract :

Introduction:In this study, the antifungal effect of aqueous and alcoholic extracts and peppermint essential oil on the growth of Candida albicans with fluconazole was investigated.Materials and working methods:. Candida albicans fungal samples were isolated by performing microscopic, biochemical and glucose uptake tests by API20C kit.The standard strain of ATCC14053 candidate was used to evaluate the quality control of the work. Comparison of antifungal effect of peppermint extracts with peppermint essential oil with fluconazole was performed on these isolates by disk diffusion and microdilution methods.findings:The highest relative frequency of yeast isolated from Candida albicans samples was 35% in biochemical detection method and 38% of total samples in PCR-RFLP method. The mean diameter of non-growth halos was 1 mm for aqueous and alcoholic extracts of Peppermint, 23.4 mm for Peppermint essential oil and 26.16 mm for fluconazole.The standard deviation of peppermint alcoholic extract in MFC test was 3.55; While for fluconazole it was 3.68. Given that statistically the smaller the standard deviation, the better the performance; Therefore, peppermint alcoholic extract had a better performance than fluconazole. These results indicate better performance and significant difference between peppermint alcoholic extract compared to fluconazole (P ≤ 0.05).Conclusion:According to the results, the alcoholic extract of peppermint can be used as a suitable drug candidate with better antifungal performance than fluconazole in the pharmaceutical industry.