Effects of hydroalcoholic extract of Achilla millefolium and Salvia officinalis against human gastric Cancer cells

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Omid Sofalian ¹ , naser zare ² , Saeed Latifi ³ , Sara Motalebinia ⁴ , Kobra Hasanpour ⁵

1 - Scientific staff
2 - scientific staff
3 - Scientific staff
4 - Post Graduated student
5 - Post graduated student

Received: 2019-04-28 Accepted : 2020-12-25 Published : 2020-08-22

Keywords: ethanolic extract, salvia, Achillea, Gastric cancer cells (AGS),

Abstract :

Treatment of cancer by medicinal plants has long been of interest to researchers. In the meantime, some plant species contain substances that, through apoptosis or necrosis, can inhibit or eliminate cancer cells. The family of mint and chicory are among the medicinal plants that have many biological effects.The purpose of this study was to investigate the effect of Pennyroyal and Artemisia extract on gastric cancer cell line (AGS).In this study, aerial parts of plants were extracted. AGS cells were treated with the concentrations of Hydroatanolic extract (50-1000 g / ml) at 24, 48 and 72 hours intervals. Cell motility was estimated using MTT method and the rate of induction of apoptosis by flow cytometry with The Annexin-V .The results of MTT assay showed strong and dependent inhibitors of the concentration of cancer cell proliferation by various extracts of Achillea and Salvia. These extracts have a doseand time dependent anti cancer effect on AGS cells. In the study of apoptosis in treated cells, the extract of the Pennyroyal was more effective. Due to the cytotoxic effects of hydrotanolic extracts on AGS cells, these plants can be used as potential option for further studies on cancer treatment. Therefore, the purification of the active ingredient in these extracts and the determination of their effect mechanism is recommended.