The effect of glucose, sucrose, lactose carbon sources on the growth of Alcaligenes eutrophus for the production of polyhydroxybutyrate
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
atefeh
farjadmanesh
1
(Master of chemical engineering-biotechnology, Shahid Bahonar university of Kerman, Engineering faculty, Chemical engineering group)
seyed ahmad
ataei
2
(Associate Professor, Shahid Bahonar university of Kerman, Engineering faculty, Chemical engineering group)
Keywords: Carbon sources, Polyhydroxyalkanoate producing bacteria, Biodegradable Plastics, Fed-Batch Cell Culture Techniques,
Abstract :
Background & Objectives: Polyhydroxyalkanates are a group of polymers that are produced by many bacteria when they enter the growing phase in the presence of mineral sources. The Objective of this study is investigattion of the different culture containing carbon sources of glucose, sucrose, lactose, combination of these carbon sources, on the growth of Alcaligenes eutrophus to produce biodegradable polymers of hydroxy butyrate-valerate. Materials & Methods: In this study Alcaligenes eutrophus was identified In order to producing of hydroxybutyrate-valerate and investigation of the effect of the different carbon sources on the production. To perform experiments, in fed-batch mode, acetic acid and propionic acid combination with acetic acid as a source of volatile fatty acid was added to the culture in a stepwise manner. The culture with inoculated bacteri were transferred to incubator at 32°C, 120 rpm and retention time of 72 hours, for to incubation and polymer production. Results: The results of this study showed that the source of glucose as carbon with acetic acid produced the highest amount of hydroxybutyrate-valerate (HB= 3.4860 g/l and HV=0.7940 g/l). Also, the lowest amount of hydroxybutyrate production (HB= 2.3124 g/l) is Because of using sucrose as the carbon source and the combination of acetic acid and propionic acid. Conclusion: The results showed that Alcaligenes eutrophus used carbon source of glucose and fatty acid source of acetic acid more than other sources of carbon
_||_