Study of relation on breast cancer and rs3803662 polymorphism of TOX3 gene in Iranian female population by Tetra Arms PCR

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

elham  siasi ¹ (Islamic Azad University, North Branch Tehran)
Bahare  Ghane ² (Department of Genetics, Faculity of science, North Tehran Branch, Islamic Azad University, Tehran, Iran.)
fatemeh  ashrafi ³ (Department of Genetics, Faculity of science, North Tehran Branch, Islamic Azad University, Tehran, Iran)

Received: 2018-11-22 Accepted : 2019-06-10 Published : 2019-08-23

Keywords: breast cancer, Tetra Arms PCR, rs3803662 Polymorphism, TOX3 Gene,

Abstract :

Objective: TOX3 gene plays an important role in the risk of breast cancer in females. Polymorphisms were identified in this gene, that can associated with breast cancer. The aim of this study was relation between breast cancer and rs3803662 polymorphism of TOX3 gene in Iranian females by Tetra Arms PCR. Materials and Methods: Blood samples were collected from 50 normal groups and 50 Breast cancer Patients. Then was extracted DNA from samples. Genotype frequency of this polymorphism in TOX3 gene were determined by using Tetra Arms PCR. Results: Genotyp frequency for TT, TC and CC in normal groups was 10%, 88% and 2%, and For patient groups was 6% , 50% and 44%, respectively. Frequency of CC recessive homozygous genotype of rs3803662 polymorphism in TOX3 gene between normal groups and breast cancer patients was statistical significant difference (P < 0.05). Conclusion: This research for first time was studied relation between the risk of breast cancer and presence of rs3803662 polymorphism in TOX3 gene in Iranian females. The results of this study was showed that presence of rs3803662 polymorphism in TOX3 gene, as genetic marker, can associated with breast cancer in Iranian female.