Comparative measurement of proline content in dried and fresh forms of Vitis vinifera L., Portulaca oleracea L. and Rosmarinus officinalis L. by different solvents
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Razieh Taghavizad
1
(Department of biology, Yadegar-e Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.)
Roya Ahmadi
2
(Department of chemistry, Yadegar-e Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.)
Naser Rahim
3
(M. Sc in chemistry, Yadegar-e Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.)
Keywords: free amino acid, ̏ Portulaca̋, ̏Proline̋, ̏Rosmarinus̋, ̏Vitis̋,
Abstract :
The role of proline has been proven in improving joints function and cell cycle regulation. Thus, achieving plants that have the highest proline content is important. The leaves of Vitis vinifera, Portulaca oleracea and Rosmarinus officinalis were collected in autumn. Absorption was determined at 520nm in spectrophotometer with proline standard and solutions. For measurement the total amino acids content, absorption was determined at 570nm in spectrophotometer with glycine standard. Mann-Whitney U and Friedman tests and SPSS were used. Comparisons of proline contents by different solvents, amino acids and ratio of proline to amino acids in dried and fresh forms of species showed that more proline was extracted in all fresh tissues with ortho xylene and among them the highest amount of proline belonged to fresh R. officinalis with 14.864±0.544 μg/ml. In most samples of dried tissues, more proline was extracted in measurement by toluene solution, and the highest belonged to dried P. oleracea with 10.291±0.202 μg/ml. The highest average of total free amino acids was 82.663±0.325 μg/ml in fresh R. officinalis and 68.871±0.679 μg/ml in dried V. vinifera. The comparison of the two solutions function was not significant, so it is possible to use ortho xylene instead of toluene and reduce the risks. It seems that the physiological process of response to drought stress continues with proline synthesis after harvesting and thus it plays a role in development of the reproductive phase.
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