Comparison of Ginger(Zingiber officinale) Hydroalcoholic Extract on the Viability of Cancer Cells and Embryonic Fibroblast Cells

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Elham Hoveizi ¹ (Department of biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran/Stem cells and transgenic technology research center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran)
Tayebeh Mohammadi ² (Department of biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran/Stem cells and transgenic technology research center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran)

Received: 2018-02-12 Accepted : 2018-07-11 Published : 2018-08-23

Keywords: Cytotoxicity, Ginger hydroalcoholic extract, lung cancer cell, Embryonic Fibroblast,

Abstract :

Lung cancer is the most common cause of cancer related death. Ginger is an edible and medicinal plant having important health benefits including anticancer activity. The aim of this study was evaluation and comparison of the effect of hydroalcoholic extract of ginger on lung cancer cells and normal mouse embryonic fibroblasts. Human lung cancer cell line A459 and normal mouse embryonic fibroblasts were cultured in vitro and treated with different concentrations of ginger extract for 24, 48 and 72 hours. The MTT assay was used to determine cell viability. Ginger extract in concentrations of 1800 and 2000 µg/ml had killed both of cells at 72 hours after treatment and caused morphological changes in cells which were more obviously in cancer cells. Ginger extract in concentrations of 1000, 1200, 1400, 1800 and 2000 µg/ml killed cancer cells more than embryonic cells(P<0.05). Cytotoxicity effect of ginger on lung cancer cells was more than its effect on normal mouse embryonic fibroblasts and can be regarded as a safe anticancer medicine.

References:

[1]     Abdullah S, Z.S., Murad NA, Makpol S,  Ngah ZW, Yusof YAM. 2010 Ginger extract (Zingiber officinale) triggers apoptosis and G0/G1 cells arrest in HCT 116 and HT 29 colon cancer cell lines African journal of biochemistry research. 4(4): 134-142.

[2]     Azadmehr A, Hajiaghaee A, Baradaran B, Haghdoost-Yazdi H. Apoptosis Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via Mitochondrial Intrinsic Pathway. Adv Pharm Bull 2015. 5(3):443-446

[3]     Chang W S, C.Y.H., Lu F J, Chiang H C. 1994. Inhibitory effects of phenolics on xanthine oxidase. Antican Res. 14: 501-506.

[4]     Guo H, Guan H, Yang W, Liu H, Hou H, Chen X et al. Pro-apoptotic and anti-proliferative effects of corn silk extract on human colon cancer cell lines. ONCOLOGY LETTERS 2017. 13: 973-978.

[5]     Ghayur MN, G.A. 2005. Pharmacological basis for the medicinal use of ginger in gastrointestinal disorders. Dig Dis Sci. 50(10): 1889-1897.

[6]     Housman G, B.S., Heerboth S,  Lapinska K, Longacre M etal. 2014. Drug Resistance in Cancer: An Overview Cancers (Basel). 3(6): 1769–1792.

[7]     Luijsterbur MS, A.H. 2011. Chromatin and the DNA damage response: The cancer connection. Molecular Oncology. 5(4): 349-367.

[8]     Marx W, M.D., McCarthy AL, Bird R, Ried K, Chan A, Isenring L. 2015. The Effect of Ginger (Zingiber officinale) on Platelet Aggregation: A Systematic Literature Review PLoS One. 10(10): e0141119.

[9]     Mohammadi T, Hoveizi E. 2017. comparison of the effect of rosemary hydroalcoholic extract on head and neck cancer cells(HN5) and murine neural progenitor cells viability. developmental biology. 9(3): 13-22.

[10]  Mohammadi T, Hoveizi E.2018. Comparison of anti proliferative effect of cinnamon (Cinnamomumzeylanicum) hydroalcoholic extract with cyclophosphamide medicine on A459 Cancer Cells. Razi Journal of Medical Sciences. 25(167): 22-29.

[11] Moheghi N, T.a.J, Brook A. 2011. The Cytotoxic Effect of Zingiber Afficinale in Breast Cancer (MCF7) Cell Line. Ofogh-e-Danesh. 17(4): 18-33.

[12] Nagasawa H, W.K., Inatomi H. 2002. Effects of bitter melon (Momordica charantia l) or ginger rhizome  (Zingiber offifinale rosc) on spontaneous mammary tumorigenesis in SHN mice. Ame J Chin Med. 30: 195-205.

[13] Rahmani AH, A.s.F., Aly SM. 2014. Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. Int J Physiol Pathophysiol Pharmacol. 6(2): 125–136.

[14] Remesh A. 2012. Toxicities of anticancer drugs and its management. International Journal of Basic & Clinical Pharmacology. 1(1): 2-12.

[15] Safarzadeh E, S.S., Baradaran B. 2014. Herbal medicine as inducers of apoptosis in cancer treatment. Adv Pharm Bull. 4:  421–427.

[16] Sosa V, M.T., Somoza R, Paciucci R, Kondoh H, Leonart ME. 2013. Oxidative stress and cancer: An overview. Ageing Research Reviews. 12(1): 376-390.

[17]  Surh Y.1999. Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances. Mutation Res. 428: 305-327.

[18] Yoshimi N, W.A., Morishita Y, Tanaka T, Sugie S, Yamahara J, Mori H. 1999. Modifying effects of fungal and herb metabolites on azoxymethane-induced intestinal carcinogenesis in rats. Jap J Cancer Res. 83(2): 1273-1278.