Cytotoxic Effect of Girard-T Reagent-based Schiff Bases and their Dimethyltin (IV) Complexes on Human Colon Cancer Cells

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

elham hoveizi ¹ , zynab ansariasl ²

1 - Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran/Stem cells and transgenic technology research center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 - Department of Chemistry, College of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Received: 2017-11-30 Accepted : 2018-06-23 Published : 2018-05-22

Keywords: &quot, Anti-cancer&quot, , &quot, Apoptosis&quot, , &quot, Cell viability&quot, , &quot, Stem cells&quot, ,

Abstract :

Despite many efforts, no definitive treatment is yet knowing for cancer. Therefore, research on new compounds that have anti-cancer properties is especially important. The aim of this study is investigation the cytotoxic effect of Girard-T reagent-based Schiff bases and their complexes on HT29 cancer cells. HT29 cells was cultured in DMEM/F12 medium containing FBS 10% and antibiotics 1% then the effects of ligands and their complexes with the concentration of 0.1, 1 and 5 mg/ml were surveyed on these cells in 1, 2, and 3 days. Growth, proliferation and morphological changes were photographed using an inverted microscope. MTT assay and DAPI staining were used to quantify cell viability. With concentrations of 0.1, 1, and 5 mg/ml of [SnMe2(L2)] (the effective component) the survival were decreased to 34, 24 and 18% after 72h(P<0.05). Encountering cells with complex caused to induce apoptosis in relation with concentration so that, the cell viability of cells treated with concentrations of 5 mg/ml (the effective concentration) of (H2L1) ،(H2L2) ،[SnMe2(L1)]،[SnMe2(L2)] was 22, 18, 29, and 22 respectivly after 72h and the highest apoptosis (82%) happened in 5mg/ml of SnMe2(L2). The synthetic components have a toxic and ant-proliferation effect on HT29 in relation with concentration.

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