Induction Of NMRI Mice Mesenchymal Stem Cell Differentiation Into Endothelial Progenitor Cells By Placental Extract

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

maryam sadat Nezhadfazel ¹ , Kazem Parivar ² , nasim HAYATI ³ , mitra heydari ⁴

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - گروه زیست شناسی، دانشکدة علوم، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biology, Islamic Azad University, Parand, Iran

Received: 2017-06-23 Accepted : 2017-07-07 Published : 2017-05-22

Keywords: growth factors, MTT Test, Cell Markers, Cell Cycle, Flowcytometry VEGF,

Abstract :

Stem cells are undifferentiated pluripotent cells, can produce different tissues and organs of the body. Stem cells have two features, self-renewal and differentiation. Of the most important adult stem cells are mesenchymal stem cells. These cells are capable of proliferation and differentiation with induction in the presence of variety of growth factors. Since the placenta extract contains a large amount of VEGF, therefore placenta extract was used to induce the differentiation of omentum mesenchymal stem cells.The omentum mesenchymal stem cells were separated and cultured and cell-specific markers CD90, CD44, CD73, CD105 and CD34 markers were tested by flowcytometry on them. The extract of the placenta was prepared and after the MTT test, we selected a 20% viability percentage. After 21 days the presence of mesenchymal stem cells omentum in medium and 20 percent of placenta extract, mesenchymal cells were differentiated into progenitor endothelial cells. The expression of CD90, CD73 and CD105 markers was negative and expression of CD31, FLK1 and CD34 markers was positive. G1 phase of the cell cycle in 2st week of prolonged, compared to the control group, this result showed that the cells were exited from the proliferative phase into differentiation phase.Conclusion: Omental mesenchymal cells in adjacent to the placenta extract, differentiate into endothelial progenitor cells.

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