The effects of silicate and silicon nanoparticles on seed germination and growth parameters of fenugreek (Trigonella foenum-graecum L.)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Sanam Nazaralian ¹ ( kharazmi university )
Ahmad Majd ² ( Department of Biology, Faculty of Science, North Tehran Branch of Islamic Azad University )
Saeed Irian ³ ( Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University )
Farrokh Ghahremaninejad ⁴ ( Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University,Tehran )
Farzaneh Najafi ⁵ ( Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran )
Maria Greger ⁶ ( Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91 Stockholm, Sweden )

Received: 2016-05-23 Accepted : 2016-09-14 Published : 2016-09-21

Keywords: germination, Fenugreek, silicon, Seedling development, Silicon nanoparticles,

Abstract :

The addition of silica increase plant biomass growth. The aim of this study was to find out if silicate and nano silica (SiNP) influence the growth processes in fenugreek (Trigonella foenum-graecum L.) similarly. Plants were grown from seed with 0-2.5 mM of sodium silicate and SiNP in hydroculture for up to 30 days. Germination, seedlings development and other growth parameters in fenugreek plantsas well as Si uptake was examined. The results showed that Si concentration in root and shoot increased with Si addition. Seed germination and vitality index increased with Si at day 4 after seeding, but the effect was then levelled out. Seedlings length and fresh weight (FW), whole plant length and leaf area increased while dry weight (DW) and DW: FW ratio of shoot and radicle length decreased with silicate and SiNP treatment. The root central cylinder increased in diameter in young roots and the lignified tissue of the endodermis cells increased in thickness. The effects by silicate and SiNP was similar but the increase in whole plant length and cell wall thickness and the decrease in DW: FW ratio of shoot and radicle length was slightly more pronounced with SiNP than with silicate.In conclusion, both silicate and SiNP in general have similar effects on plant growth and therefore SiNP can be used instead of silicate.

References:

[1] Adams F (1980) Interactions of phosphorus with other elements in soils and in plants. The role of phosphorus in agriculture. American Society of Agronomy 655-680. [2] Epstein E (2009) Silicon: its manifold roles in plants. Annals of Applied Biology 155:155160.
[3] Greger M, Landberg T (2015) Silicon reduces cadmium and arsenic levels in field-grown crops. Silicon. DOI 10.1007/s12633-015-9338z [4] Hwang SJ, Hamayun M, Kim HY, Na CI, Kim KU, Shin DH, Lee IJ (2007) Effect of nitrogen and silicon nutrition on bioactive gibberellin and growth of rice under field conditions. Journal of Crop Science and Biotechnology 10:281-286.
[5] Imtiaz M, Rizwan MS, Mushtaq MA, Ashraf M, Shahzad SM, Yousaf B, Saeed DA, Rizwan M, Nawaz MA, Mehmood S, Tu S (2016) Silicon occurrence, uptake, transport and mechanisms of heavy metals, minerals and salinity enhanced tolerance in plants with future prospects: A review. J. Environ. Manag 183:521–529. [6] Keller C, Guntzer F, Barboni D, Labreuche J, Meunier JD (2012) Impact of agriculture on the Si biogeochemical cycle: input from phytolith studies. Comptes Rendus Geoscience 344:739746. 62
[7] Kuai J, Sun Y, Guo C, Zhao L, Zuo Q, Wu J, Zhou G (2017) Root-applied silicon in the early bud stage increases the rapeseed yield and optimizes the mechanical harvesting characteristics. Field Crops Research 200:8897.
[8] Kupfer C, Kahnt G (1992) Effects of the application of amorphous silica on transpiration and photosynthesis of soybean plants under varied soil and relative air humidity conditions. Journal of Agronomy and Crop Sciences 168:318-325.

[9] Kvet J, Necas J, Kubín Š (1966) Measurement of leaf area. Methods of Studying Photosynthetic Production of Plants. Academia Praha 315-334.

[10] Lavinsky AO, Detmann KC, Reis JV, Ávila RT, Sanglard ML, Pereira LF, DaMatta FM (2016) Silicon improves rice grain yield and photosynthesis specifically when supplied during the reproductive growth stage. Journal of Plant Physiology 206: 125-132.
[11] Lee SK, Sohn EY, Hamayun M, Yoon JY, Lee IJ (2010). Effect of silicon on growth and salinity stress of soybean plant grown under hydroponic system. Agroforestry systems 80: 333-340.
[12] Lin D, Xing B (2007) Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environmental Pollution 150: 243-250.
[13] Lukačová Z, Švubová R, Kohanová J, Lux A (2013) Silicon mitigates the Cd toxicity in maize in relation to cadmium translocation, cell distribution, antioxidant enzymes stimulation and enhanced endodermal apoplasmic barrier development. Plant Growth Regulation 70:89103.
[14] Ma CC, Li QF, Gao YB, Xin TR (2004) Effects of silicon application on drought resistance of
cucumber plants, Soil Science and Plant Nutrition 50:623-632.

[15] Mozaffarian V. 1997. A dictionary of Iranian plant names. Iranian Contemporary Culture Press, Tehran 756-757.

[16] Nazaralian S, Majd A, Irian S, Najafi F, Ghahramaninejad F, Landberg T, Greger M (2017) Comparison of silicon nanoparticles and silicate treatments in fenugreek. Plant Physiology and Biochemistry http://dx.doi.org/10.1016/j.plaphy.2017.03.009

[17] Ouzir M, El Bairi K, Amzazi S (2016) Toxicological properties of fenugreek (Trigonella foenum graecum). Food and Chemical Toxicology 96:145-154.

[18] Savant NK, Korndörfer GH, Datnoff LE, Snyder GH (1999) Silicon nutrition and sugarcane production: a review. Journal of Plant Nutrition 22:1853-1903.
[19] Shi Y, Zhang Y, Yao H, Wu J, Sun H, Gong H (2014) Silicon improves seed germination and alleviates oxidative stress of bud seedlings in tomato under water deficit stress. Plant Physiology and Biochemistry 78:27-36.
[20] Siddiqui MH, Al-Whaibi MH (2014) Role of nano-SiO 2 in germination of tomato (Lycopersicum esculentum seeds Mill.). Saudi journal of biological sciences 21:13-17. [21] Sitharaman B ed (2016) Nanobiomaterials handbook. CRC Press. [22] Vaculík M, Lux A, Luxová M, Tanimoto E, Lichtscheidl I (2009) Silicon mitigates cadmium inhibitory effects in young maize plants. Environmental and Experimental Botany 67:52-58.
[23] Zhu Y, Gong H (2014) Beneficial effects of silicon on salt and drought tolerance in plants.Agronomy for sustainable development. 34: 455-472.