Phenetic study of Trifolium species using pollen grain characters

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

فهیمه سلیم پور ¹ , Golale Mostafavi ² , شادی حاج رسولیها ³ , فریبا شریف نیا ⁴

1 - عضوهییت علمی گروه ریست شناسی دانشگاه آزاد اسلامی- تهران شمال
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - عضوهییت علمی دانشگاه آزاد اسلامی واحدرودهن
4 - عضوهییت علمی دانشگاه آزاد اسلامی واحدتهران شمال

Received: 2016-04-18 Accepted : 2016-07-13 Published : 2016-09-21

Keywords: Iran, pollen, Trifolium,

Abstract :

Iran is one of centers of diversity for Trifolium species in Asia with 49 species in 6 sections . There are high similarities between species specially in calyx tube, so taxonomic identification are difficult. In this research, major pollen grain characters were studied in 34 species of this genus by used Scaning Electron Microscopy (S.E.M). The results showed that, pollen grains are trizoncolporate, the equatorial view was oblong to circular shapes and sculpture of exine were reticulate, irregular reticulate, perforate, scrobiculate and foveolate. T. tomentosum and T. bullatum was distinguished with their sculptures, also in T. campestre exine surface was reticulate but in T. aureum was regulate. In the other hand, T. pilulare has the most different sculpture between other species. Based on cluster analysis, two main groups were detected. One cluster is containing both species of Vesicaria and Trifolium sections and another clade divided into three subclades, but the species of all sections are complex .Our results showed that Trifolium is the heterogenous with variation in morphology and pollen grain.

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