Evaluation of the adverse effects of cadmium on rat lung tissue and the preventive roles of vitamin D and magnesium through regulation of VEGF and BMP-4 signaling pathways
Subject Areas :Paria Amanpour 1 , Zohre Eftekhari 2 * , Akram Eidi 3 , Parvin Khodarahmi 4
1 - Research Sciences Branch, Islamic Azad University, Tehran, Iran
2 - Biotechnology Department, Institute of Pasteur, Tehran, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 - Department of Biology, Parand Branch, Islamic Azad University, Tehran, Iran
Keywords: Rat, Lung, Cadmium, Vitamin D, Magnesium,
Abstract :
Background: Cadmium (Cd) exposure occurs primarily through ingestion of contaminated food and water, inhalation, and smoking. Magnesium plays an essential role in the synthesis and metabolism of vitamin D. Objective: This study aimed to investigate the protective mechanisms of vitamin D and magnesium against cadmium-induced lung damage. Methods: This study investigated the effects of cadmium on rat lungs and possible defense against cadmium-induced lung injury following vitamin D and magnesium administration. Body weight, toxicity index, blood parameters, liver function, and lung histology were evaluated in this study, along with liver enzyme levels, P53, VEGF, and BMP-4 gene expression levels. Results: After cadmium exposure, a significant increase in liver enzyme levels, toxicity index, VEGF, and BMP-4 expression was observed. The results showed that treatment with vitamin D, magnesium, and vitamin D + magnesium reduced toxicity index, VEGF, and BMP-4 protein expression. Histopathological findings showed increased inflammatory cell infiltration, mucus accumulation, and alveolar wall thickness in the lung tissue, and this trend was reversed after cadmium injection. Conclusion: The present study shows that the combination of vitamin D and magnesium can exert significant protective effects against cadmium-induced pulmonary toxicity through the regulation of VEGF and BMP-4 pathways. These findings may open the way for the development of new therapeutic approaches.
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