The role of orexin 1 and 2 receptors in the accumbens area on passive avoidance memory processing in rats

Subject Areas : Journal of Comparative Pathobiology

laleh zokaei ¹ , esmaeil akbari ² , Morteza Zendehdel ³ , Vahab Babapour ⁴

1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Physiology and Pharmacology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3 - ‎Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran‎
4 - دانشگاه تهران

Received: 2024-03-01 Accepted : 2024-04-27 Published : 2024-03-05

Keywords: Orexin Receptors, Memory, Accumbens Area, Rat ,

Abstract :

Orexin is a neuropeptide that is localized mainly to neurons within and around the lateral hypothalamus and binds to two orexin receptors type 1 and 2. The presence of orexinergic neurons in the accumbens area has also been proven. Previous studies have shown the role of orexin in the dorsal raphe nucleus and hippocampus in learning and memory processes, but so far no study has focused on the effects of orexin in the accumbens area on memory. In the present study, the role of orexin 1 and 2 receptors in the accumbens area on consolidation and retrieval of passive avoidance memory in rats was investigated. For this purpose, 24 Wistar rats were prepared and 4 experiments were designed and performed in the shuttle box environment. After training, the test groups of each experiment were injected with control solution (DMSO), type 1 orexin receptor antagonist (SB334867-A) and type 2 orexin receptor antagonist (TCS-OX2-29) through a cannula into the accumbens area. The results showed that in the passive avoidance memory consolidation phase, the administration of SB334867-A significantly reduced the duration of staying in the light part and the first time to enter the dark part of the box compared to the control group (p<0.05), while Administration of TCS-OX2-29 did not have a significant effect (p>0.05). In the passive avoidance memory retrieval phase, the administration of SB334867-A and TCS-OX2-29 did not cause a significant change in the duration of staying in the light part and the first time of entering the dark part of the box (p>0.05). According to the results, it is possible that the functional deletion of type 1 orexin receptor affects and disrupts the process of passive avoidance memory consolidation.

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