Evaluating the Different Levels of Diaminozied on the Five Mini-Tuber Potato Cultivars produced by Tissue Culture under Greenhouse Condition

Subject Areas : Journal of Crop Ecophysiology

مهدی Soleimani Agdam ¹ , M. Valizadeh ² , A.A. Imani ³ , D. Hassan Panah ⁴ , Sh. Azizi ⁵

1 - Plant Breeder Ms.c. Student, Ardabil Branch, Islamic Azad University, Ardabil, Iran
2 - Prof., Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Assistant Prof., Ardabil Branch, Islamic Azad University, Ardabil, Iran
4 - Assistant Prof., Agricultural and Natural Resources Research Center, Ardabil, Iran
5 - Former Ms.c. Student, Young Researchers and Elites Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2012-01-25 Accepted : 2013-10-30 Published : 2013-05-22

Keywords: tissue culture, Potato, Mini-tuber, Diaminozied,

Abstract :

To evaluate the effect of different levels of diaminozied on the potato cultivars mini-tubers produced by tissue culture under greenhouse condition a factorial an experiment on the base of completely randomized design in three replications was performed at the Ardabil Villkige Company Greenhouse in 2010. The first factor consisted of four levels of diaminozied, as plant growth regulator, (0, 40, 80 and 120 mg.L-1) and the second factor of five cultivars of virus-free potato (Satina, Savalan, Marfona, Agria and Cesear). Virus-free plantlets were transferred to isolated greenhouse and planted in 20×20×15 cm in plastic pots beds made by 1:1 volume of Biolan peat moss and perlite. During growth period traits like plant height, leaf and stem number per plant, mini-tuber weight and number per plant and square meter, and mini-tuber size were average measured. The analysis of variance showed significant differences among different levels of diaminozied for leaf number per plant, mini-tuber weight and its number per square meter, tuber average size in all cultivars under study. The interaction of diaminozied by cultivars showed significant difference as to leaf number per plant, mini-tuber weight, its number per square meter and average tuber size. Cesear and Marfona cultivars produced higher mini-tuber weight and tuber number per square meter by applying 40 mg.L-1 diaminozied. The correlation was significant and positive between mini-tuber number per square meter with mini-tuber weight per square meter and root length. It was also significant and positive between mini-tuber weight per square meter with plant height and average mini-tuber size. The results of multi regression analysis indicated that mini-tuber weight per square meter, average tuber size, plant height and root length were effective on changes of the mini-tuber number. The highest positive direct effect on number of mini-tuber per square meter was due to the plant height and the less negative direct effect to the mini-tuber weight per square meter.