Study of effects of different plant growth regulators on explant's establishment of Aloe vera (Aloe barbadensis Mill.)

Subject Areas : Genetic

A.B Barzgar ¹ , مرضیه Shafiee HajiAbad ² , محمود Abdy ³

1 - Scientific member of Islamic Azad University- Kashmar branch
2 - Instructor of Islamic Azad University- Kashmar branch (Coresponder)
3 - Lab assistant of Azad University- Kashmar branch.

Received: 2009-04-24 Accepted : 2009-09-17 Published : 2010-03-16

Keywords: In vitro culture, BA, aloe vera, Shoot proliferation, IAA, shoot tip,

Abstract :

Aloe (Aloe barbadensis Mill) is one of the most important medicinal plants belonging to Liliaceae family that nowadays is propagated by in vitro culture in high-scale propagation. Because of the impotance of the establishment stage in in vito culture of this plant, this study was conducted to investigate the best media culture for Aloe explants establishment. First, the prolifration rate of two kind of explants (Shoot tips and auxiliary buds) were evaluated on 8 media cultures and shoot tips selected as the best explants. Then, in order to determine the best media culture an experiment was conducted in factorail design with two factors: auxins in four levels (1 mgl-1 IAA, 0.5 mgl-1IAA, 1 mgl-1IBA, 0.5 mgl-1IBA) and cytokinins in two levels (BA and Kinetin at 1 mgl-1) in a complete randomised design with 3 replications. After 35 days the highest rate of prolifration earned on the media contained 0.5 mgl-1 IAA and 1 mgl-1 BA with 6.0 shoots per explant and 2.3 cm length, that is higher and faster than other related reports. Since on the stablishment stage of in vitro culture regnaration of the highest shoot number per explant with small size has considerable attention, this protocol suggestes an appropriate method for establishment of Aloe explants.

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