Essential oils composition and antimicrobal effects of essential oils and methanol extracts of Salvia multicaulis.Vahl against Xanthomonas translucens pv. Cerealis

Subject Areas : Genetic

امیر Azizi ¹ , A.M Azizi ² , غلامرضا Azizi ³

1 - Islamic Azad University branch (Sama), Arak and Member of young researchers club Islamic Azad University. Arak
2 - Institute of standards & Industrial research of Markazi provinces, Arak.
3 - Sc. student of Plant protection organization Markazi provinces

Received: 2008-08-24 Accepted : 2009-06-08 Published : 2009-12-16

Keywords: Essential oils, Medicinal plant, Methanol extracts, Salvia Multicalis Vahl. Anti microbial effect, Markazi Provinces,

Abstract :

Salvia multicaulis Vahl. is one of the important unique medicinal plants the it use has been known from past by native people. Ground parts of plant collected were submitted to water distillation for 3 h using a Clevenger-type apparatus and sample were extracted by using a scxhlet apparatus for about 6 h. Constituents of essential oils and methanolic extracts of Salvia multicaulis Vahl were collected from arak and analysed by GC & GC-Mass. Twenty-tree componets representing 98.2% of the oil, were identified. The main constituents of the oil and extracts were 1,8 cyneol (24.78%) and camphor (17.95%). Then the effects of different concentrations of essential oil and methanolic extracts prepared from leaves of Xanthomonas translucens pv. Cerealis were tested. In this investigation use of controller and different dilutions of essential and extracted of Salvia multicaulis Vahl were treatments. The disc diffusion method was employed for the determination of antimicrobial activites of essential oil and methanolic extracted. At concentration used inhibition zone was 12.5 mm indiameter wholl applayed extracted solution for well and disc method. But inhibition zone was 8 mm indiameter at each two method. The essential oil (60 percent) and the dilutions of methanolic extracts (80 percent) on Xanthomonas translucens pv. Cerealis had MBC efficacy.The dilutions of essential oil 20 percent also the dilutions of methanolic extracts 20 percent on Xanthomonas translucens pv. Cerealis had MIC efficacy.

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