The effect of acidity at limited irradiance on survival, growth, pigment composition and ammonium liberation of soil cyanobacterium Nostoc sp., collected from paddy-fields of Golestan province
Subject Areas : Geneticفریبا Amirlatifi 1 , sh Shokravi 2 , مریم Safaei 3 , زهرا Hosseini kolbadi 4
1 - Dept. Biology and Researching, Young Club Islamic Azad University Gorgan Branch, Gorgan, Iran
2 - Dept. Biology, Islamic Azad Univ.- Gorgan Branch, Gorgan, Iran
3 - Dept. Biology and Researching, Young Club Islamic Azad University Gorgan Branch, Gorgan, Iran.
4 - Dept. Biology and Researching, Young Club Islamic Azad University Gorgan Branch, Gorgan, Iran.
Keywords: carbon dioxide, Acidity, irradiance, Nostoc, Cyanobacterium, Pigment composition, Ammonium liberation,
Abstract :
Cyanobacterium Nostoc sp. can be considered as an interesting microorganism for the ability of excretion buffering compounds. With respect to possible applied potential of this strain, this ability has been studied under limited irradiance. Soil samples were collected from paddy-fields during one year. At the first step, different acidities (pHs 5,7,9) were studied under limited carbon dioxide condition. Results showed that in all treatments, organism tends to enter logarithmic phase of growth until third day, but acidic condition cause sharp decline on growth after this. Highest specific growth rate belong to alkaline condition (pH 9). For the next step, two alkaline condition near to pH 9 (pHs 8.5 and 9.5), were selected to compare. Results showed that specific growth rate was significantly less than pH 9, and even continuous aeration couldn’t be able to enlarge specific growth rate. Pigment composition can be rearranged at different acidities and phycobilisome systems cause viability and growth under acidity fluctuations and limited irradiance in addition of low carbon dioxide concentration. Ammonium liberation showed the highest rate at pH 5 and day two too.
خاوری نژاد، ر.، ریاحی، ح. و شکروی، ش. (1381). بررسی تأثیر شوری، اسیدیته و عدم تلقیح دی‌اکسیدکربن بر رشد، وضعیت رنگیزه‌ای و فرکانس هتروسیست سیانوباکتریوم Nostoc sp. مجله علوم پایه دوره دکترای دانشگاه آزاد اسلامی، شماره 41 بهار.
سلطانی، ن.، خاوری نژاد، ر.، طباطبایی یزدی، م.، شکروی، ش. و فرناندز والینته، ا. (1384). بررسی خواص آنتی میکروبیال و فیزیولوژی سیانوباکتری‌ها در محیط‌های افراطی، پایان‌نامه دکترای تخصصی، گروه زیست شناسی، دانشکده علوم، دانشگاه تربیت معلم تهران.
شکروی، ش.، سلطانی، ن. و بافته‌چی، ل. (1381). تدوین تکنولوژی استفاده از سیانوباکتری‌ها به عنوان کود بیولوژیک در شالیزارها، شورای عالی تحقیقات نهاد ریاست جمهوری (طرح ملی) مجری پژوهشکده علوم پایه کاربردی، جهاد دانشگاهی، دانشگاه شهید بهشتی.
شکروی، ش. و ساطعی، آ. ( 1382). بررسی پتانسیل سیانوباکتری به منظور تلقیح در شالیزار، گزارش طرح پژوهشی، معاونت پژوهشی دانشگاه آزاد اسلامی واحد گرگان.
Adams, D.J. and Duggan P.S. (1999). Heterocyst and Akinete differentiation in cyanobacteria. Tansley Review No 107 New Phytologist 144: 3-33
Anagnostidis, K. and Komarek J. (1990). Modern approaches to the classification of cyanobacteria. Stigonematales. Archieves for hydrobiology supl4: Pp. 224-286
Anand, N., Radha L., Shanthakumar Hopper RS. Revathi G. and Subramanian TD. (1990). Blue-green algae as biofertilizers: Certain view points on the choice of suitable isolates-Perspective in phycology, International symposium of phycology at university of Madras, Today and Tomorrow's Publishers. New Delhi, India, PP. 383-391
Boussiba, S. (1988). Anabaena azollae as biofertilizer. In: Stadler, T.,J., Millon, M.C.Verdus, Y.Karamanos,H.Morvan and D.Christiaen (ed.), Algal biotechnology Elsevier applied science.
Desikachary, T.V. (1959). Cyanophyta. Indian council of agricultural research, monographs on Algae New Delhi, India
Geitler, L. (1932). Cyanophyceae von Europa Kryptogamen flora Akademiche Verlagsgesellschaft. Leipzig
Jensen, A. (1978). Chlorophylls and carotenoides in: Handbook of Phycological Methods, Physiological and Biochemical Methods(ed.) Hellebust,J.A. and Craigie, J.S., Cambridge University Press.
John, D. M., Whitton, B.W. and Brook, A.J. (2002). The Freshwater Algal Flora of The British Isles Cambridge University Press
Kaushik, B.D. (1988). Laboratory methods for blue-green algae-Associated Publishing Company, New Delhi, India.
Poza-Carrion, C., Fernandez-Valiente, E., Pinas, FF. and Leganes, F. (2001). Acclimation of photosynthetic pigments and photosynthesis of the cyanobacterium Nostoc sp. Strain UAM 206 to combined flactuations of irradiance, pH, and inorganic carbon availability, J.Plant Physiol, 158. Pp: 1455-1461.
Prescott, G.W. (1962). Algae of the western great lake area. W.M.C. Brown Company Pub.
Solarzano, L. (1969). Determination of ammonia in natural waters by the phenol-hypochlorite method. Limno.oceanogr., 14, 799-801
Soltani, N., Khavari-Nejad, RA., Tabatabaei Yazdi, M., Shokravi, Sh. and Fernandez-Valiente, E. (2005b). Variation of Nitrogenase Activity, photosynthesis and pigmentation of cyanobacterium Fischerella ambigua strain FS18 under different irradiance and pH. World Journal of Microbiology and Biotechnology. Vol 22, No6, 571-576
Stal, J.S. (1995). Physiological ecology of cyanobacteria in microbial mats and other communities. New Phytol 131, 1-32
Suda, S., Watanabe, M.M., Otsuka, S., Mahakahant, A., Yongmanitchai, W., Nopartnaraporn, N., Liu, Y. and Day, J.G. (2002). Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. International Journal of Systematic and Evolutionary Microbiology, 52, 1577-1595.
Valiente, EF. and Leganes, L. (1989). Regulatory effect of pH and Incident Irradiance on the levels of Nitrogenase activity in the cyanobacterium Nostoc sp.UAM205 J.Plant Physiol.Vol.135. Pp. 623-627.
_||_خاوری نژاد، ر.، ریاحی، ح. و شکروی، ش. (1381). بررسی تأثیر شوری، اسیدیته و عدم تلقیح دی‌اکسیدکربن بر رشد، وضعیت رنگیزه‌ای و فرکانس هتروسیست سیانوباکتریوم Nostoc sp. مجله علوم پایه دوره دکترای دانشگاه آزاد اسلامی، شماره 41 بهار.
سلطانی، ن.، خاوری نژاد، ر.، طباطبایی یزدی، م.، شکروی، ش. و فرناندز والینته، ا. (1384). بررسی خواص آنتی میکروبیال و فیزیولوژی سیانوباکتری‌ها در محیط‌های افراطی، پایان‌نامه دکترای تخصصی، گروه زیست شناسی، دانشکده علوم، دانشگاه تربیت معلم تهران.
شکروی، ش.، سلطانی، ن. و بافته‌چی، ل. (1381). تدوین تکنولوژی استفاده از سیانوباکتری‌ها به عنوان کود بیولوژیک در شالیزارها، شورای عالی تحقیقات نهاد ریاست جمهوری (طرح ملی) مجری پژوهشکده علوم پایه کاربردی، جهاد دانشگاهی، دانشگاه شهید بهشتی.
شکروی، ش. و ساطعی، آ. ( 1382). بررسی پتانسیل سیانوباکتری به منظور تلقیح در شالیزار، گزارش طرح پژوهشی، معاونت پژوهشی دانشگاه آزاد اسلامی واحد گرگان.
Adams, D.J. and Duggan P.S. (1999). Heterocyst and Akinete differentiation in cyanobacteria. Tansley Review No 107 New Phytologist 144: 3-33
Anagnostidis, K. and Komarek J. (1990). Modern approaches to the classification of cyanobacteria. Stigonematales. Archieves for hydrobiology supl4: Pp. 224-286
Anand, N., Radha L., Shanthakumar Hopper RS. Revathi G. and Subramanian TD. (1990). Blue-green algae as biofertilizers: Certain view points on the choice of suitable isolates-Perspective in phycology, International symposium of phycology at university of Madras, Today and Tomorrow's Publishers. New Delhi, India, PP. 383-391
Boussiba, S. (1988). Anabaena azollae as biofertilizer. In: Stadler, T.,J., Millon, M.C.Verdus, Y.Karamanos,H.Morvan and D.Christiaen (ed.), Algal biotechnology Elsevier applied science.
Desikachary, T.V. (1959). Cyanophyta. Indian council of agricultural research, monographs on Algae New Delhi, India
Geitler, L. (1932). Cyanophyceae von Europa Kryptogamen flora Akademiche Verlagsgesellschaft. Leipzig
Jensen, A. (1978). Chlorophylls and carotenoides in: Handbook of Phycological Methods, Physiological and Biochemical Methods(ed.) Hellebust,J.A. and Craigie, J.S., Cambridge University Press.
John, D. M., Whitton, B.W. and Brook, A.J. (2002). The Freshwater Algal Flora of The British Isles Cambridge University Press
Kaushik, B.D. (1988). Laboratory methods for blue-green algae-Associated Publishing Company, New Delhi, India.
Poza-Carrion, C., Fernandez-Valiente, E., Pinas, FF. and Leganes, F. (2001). Acclimation of photosynthetic pigments and photosynthesis of the cyanobacterium Nostoc sp. Strain UAM 206 to combined flactuations of irradiance, pH, and inorganic carbon availability, J.Plant Physiol, 158. Pp: 1455-1461.
Prescott, G.W. (1962). Algae of the western great lake area. W.M.C. Brown Company Pub.
Solarzano, L. (1969). Determination of ammonia in natural waters by the phenol-hypochlorite method. Limno.oceanogr., 14, 799-801
Soltani, N., Khavari-Nejad, RA., Tabatabaei Yazdi, M., Shokravi, Sh. and Fernandez-Valiente, E. (2005b). Variation of Nitrogenase Activity, photosynthesis and pigmentation of cyanobacterium Fischerella ambigua strain FS18 under different irradiance and pH. World Journal of Microbiology and Biotechnology. Vol 22, No6, 571-576
Stal, J.S. (1995). Physiological ecology of cyanobacteria in microbial mats and other communities. New Phytol 131, 1-32
Suda, S., Watanabe, M.M., Otsuka, S., Mahakahant, A., Yongmanitchai, W., Nopartnaraporn, N., Liu, Y. and Day, J.G. (2002). Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. International Journal of Systematic and Evolutionary Microbiology, 52, 1577-1595.
Valiente, EF. and Leganes, L. (1989). Regulatory effect of pH and Incident Irradiance on the levels of Nitrogenase activity in the cyanobacterium Nostoc sp.UAM205 J.Plant Physiol.Vol.135. Pp. 623-627.