Study of ascorbate and NaCl interaction on germination, growth and enzymes antioxidant activity in Soybean (Glycine max L. cv DPX) seedling
Subject Areas : Genetic
آتنا Diansaii
1
(Department of biology, Islamic Azad university–Gorgan Branches Iran.)
مریم Niakan
2
(Department of biology, Islamic Azad university–Gorgan Branches Iran.)
آرین Sateei
3
(Department of biology, Islamic Azad university–Gorgan Branches Iran.)
Keywords: growth, Enzyme, Antioxidant, salt, soybean, Ascorbate,
Abstract :
In different stress such as salinity, strong oxidant as Reactive Oxygen Species is produced that damages to membrane structure in plant. Different antioxidant as ascorbate scavenger them. In this research Soybean (Glycine max L. cv DPX) in different concentrations of ascorbate (1,2mM) and NaCl (50mM) and the effect of them on germination percentage, radicle length and antioxidant enzymes such as catalase, peroxidase, polyphenol oxidase and ascorbate peroxidase was evaluated. The results of this research showed that in absence of ascorbate and present of NaCl activity of enzymes increased but with increasing of ascorbate, activity of them decreased. Also in present of NaCl germination decreased but in NaCl and ascorbate germination increased significantly.
لطیفی، ن. و قاسمی، م. (1377). دانهها و مصارف آنها. انتشارات دانشگاه علوم و کشاورزی منابع طبیعی گرگان.
Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata,N. (2000b). Ionic and osmotic effects of NaCl-induced inactivationof photosystems I and II in Synechococcus sp. Plant Physiol. 123, 1047–1056
Amor, N.B., Hamed, K.B.,Debez, A., Grignon, C., Abdelly, C., (2005). Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Science 168, 889–899
Arrigoni,O.(1994).Ascorbate system in plant development.J. Bioenergy.Biomember,26,407-419.
Arrigoni, O., De Tullio MC. (2000). The role of ascorbic acid in cell metabolism: between gene-directed functions and unpredictable chemical reactions. Journal of Plant Physiology 157, 481–488
Asada, K., (1997). The role of ascorbate peroxidase and monodehydroascorbate reductase in H2O2 scavenging in plants. In: Scandalios, J.G. (Ed.), Oxidative Stress and the Molecular Biology of Antioxidant Defense, Monograph Series, vol. 34. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp. 715–735
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Benavides, M.P., Marconi, P.L., Gallego, S.M., Comba, M.E.,Tomaro, M.L., (2000). Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum. Aust. J. Plant Physiol. 27, 273–278
Chance ,B., and Maehly, C. (1995). Assay ctalase and peroxidase methods enzymol. 11,764-775
Demiral, T., Turkan, I., (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany 53, 247–257
De Tullio ,MC., Paciolla, C., Dalla Vecchia,F., Rascio., N, D’Emerico, S., De Gara, L., Liso, R., Arrigoni, O. (1999). Changes in onion root development induced by the inhibition of peptidyl-prolyl hydroxylase and influence of the ascorbate system on cell division and elongation. Planta 209, 424–434
Gulzar, S.(2002). Effect of salinity on germination, dormancy, growth, and osmoregulation of perennial halophytes. Ph.D. dissertation, University of Karachi, Karachi, Pakistan
Halliwell , B., Gutteridge, JMC. (1998). iron and free radical reactions: two aspects of antioxidant protection.Trends Biochemical Science 11,375
Khan, M.A., Gul, B., (2005). Halophyte seed germination. In: Khan, M.A., Weber, D.J. (Eds.), Ecophysiology of High Salinity Tolerant Plants. Springer, Netherlands, pp. 11–30
Koroi,S. A.(1989). Gel elektrophers tische and spectralphotometris choe unter unchngen zomein fiuss der temperature auf straktur and aktritat der amylase and peroxidase isoenzyme ,physiol veg 20,15-23
Liso, R., Innocenti, AM, Bitonti, MB., Arrigoni, O. (1998). Ascorbic acid-induced progression of quiescent centre cells from G1 to S phase. New Phytologist 110, 469–471
Manoranjan Kar and Dina Bandhu Mishra. (1976). Catalase ,peroxidase and poly phenol oxidase activites during rice leaf senescence.Plant Biochemistry and Enzymology ,57,pp.315-319
Manz, B., Mu¨ ller, K., Kucera, B., Volke, F. and Leubner-Metzger, G. (2005). Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging. Plant Physiology138, 1538–1551
Noctor, G., and Foyer, CH. (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249–279
Ogawa, K., Iwabuchi, M., (2001). A mechanism of promoting the germination of Zinnia elegans seeds by hydrogen peroxide. Plant, Cell and Physiology 2, 286–291.
Smirnoff ,N., Conklin ,PL., Loewus ,FA. (2001). Biosynthesis of ascorbic acid in plants: a renaissance. Annual Review of Plant Physiology and Plant Molecular Biology 52, 437–467
Smirnoff N. (2000). Ascorbic acid: metabolism and functions of a multi-facetted molecule. Current Opinion in Plant Biology 3,229–235
|
Thomas, CE., McLean, LR., Parker, RA., Ohlweiler, DF. (1992). Ascorbate and phenolic antioxidant interactions in prevention of liposomal oxidation. Lipids 27, 543–550
Wilson, C., and Shannon ,MC. (1995). Salt-induced Na+/H+ antiport in root plasma membrane of a glycophytic species of tomato. Plant Science 107, 147–157
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لطیفی، ن. و قاسمی، م. (1377). دانهها و مصارف آنها. انتشارات دانشگاه علوم و کشاورزی منابع طبیعی گرگان.
Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata,N. (2000b). Ionic and osmotic effects of NaCl-induced inactivationof photosystems I and II in Synechococcus sp. Plant Physiol. 123, 1047–1056
Amor, N.B., Hamed, K.B.,Debez, A., Grignon, C., Abdelly, C., (2005). Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Science 168, 889–899
Arrigoni,O.(1994).Ascorbate system in plant development.J. Bioenergy.Biomember,26,407-419.
Arrigoni, O., De Tullio MC. (2000). The role of ascorbic acid in cell metabolism: between gene-directed functions and unpredictable chemical reactions. Journal of Plant Physiology 157, 481–488
Asada, K., (1997). The role of ascorbate peroxidase and monodehydroascorbate reductase in H2O2 scavenging in plants. In: Scandalios, J.G. (Ed.), Oxidative Stress and the Molecular Biology of Antioxidant Defense, Monograph Series, vol. 34. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp. 715–735
|
Benavides, M.P., Marconi, P.L., Gallego, S.M., Comba, M.E.,Tomaro, M.L., (2000). Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum. Aust. J. Plant Physiol. 27, 273–278
Chance ,B., and Maehly, C. (1995). Assay ctalase and peroxidase methods enzymol. 11,764-775
Demiral, T., Turkan, I., (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany 53, 247–257
De Tullio ,MC., Paciolla, C., Dalla Vecchia,F., Rascio., N, D’Emerico, S., De Gara, L., Liso, R., Arrigoni, O. (1999). Changes in onion root development induced by the inhibition of peptidyl-prolyl hydroxylase and influence of the ascorbate system on cell division and elongation. Planta 209, 424–434
Gulzar, S.(2002). Effect of salinity on germination, dormancy, growth, and osmoregulation of perennial halophytes. Ph.D. dissertation, University of Karachi, Karachi, Pakistan
Halliwell , B., Gutteridge, JMC. (1998). iron and free radical reactions: two aspects of antioxidant protection.Trends Biochemical Science 11,375
Khan, M.A., Gul, B., (2005). Halophyte seed germination. In: Khan, M.A., Weber, D.J. (Eds.), Ecophysiology of High Salinity Tolerant Plants. Springer, Netherlands, pp. 11–30
Koroi,S. A.(1989). Gel elektrophers tische and spectralphotometris choe unter unchngen zomein fiuss der temperature auf straktur and aktritat der amylase and peroxidase isoenzyme ,physiol veg 20,15-23
Liso, R., Innocenti, AM, Bitonti, MB., Arrigoni, O. (1998). Ascorbic acid-induced progression of quiescent centre cells from G1 to S phase. New Phytologist 110, 469–471
Manoranjan Kar and Dina Bandhu Mishra. (1976). Catalase ,peroxidase and poly phenol oxidase activites during rice leaf senescence.Plant Biochemistry and Enzymology ,57,pp.315-319
Manz, B., Mu¨ ller, K., Kucera, B., Volke, F. and Leubner-Metzger, G. (2005). Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging. Plant Physiology138, 1538–1551
Noctor, G., and Foyer, CH. (1998). Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249–279
Ogawa, K., Iwabuchi, M., (2001). A mechanism of promoting the germination of Zinnia elegans seeds by hydrogen peroxide. Plant, Cell and Physiology 2, 286–291.
Smirnoff ,N., Conklin ,PL., Loewus ,FA. (2001). Biosynthesis of ascorbic acid in plants: a renaissance. Annual Review of Plant Physiology and Plant Molecular Biology 52, 437–467
Smirnoff N. (2000). Ascorbic acid: metabolism and functions of a multi-facetted molecule. Current Opinion in Plant Biology 3,229–235
|
Thomas, CE., McLean, LR., Parker, RA., Ohlweiler, DF. (1992). Ascorbate and phenolic antioxidant interactions in prevention of liposomal oxidation. Lipids 27, 543–550
Wilson, C., and Shannon ,MC. (1995). Salt-induced Na+/H+ antiport in root plasma membrane of a glycophytic species of tomato. Plant Science 107, 147–157