Aluminum positive influences in reduction of copper toxic effects on nitrate reductase and antioxidant enzymes activities of Chlorella vulgaris Beijernick
Subject Areas : Genetic
آرین Sateei
1
(Department of biology, Islamic Azad University - Gorgan Branch)
شادمان Shokravi
2
(Department of biology, Islamic Azad University - Gorgan Branch)
نرگس Nateghi
3
(Department of biology, Islamic Azad University - Gorgan Branch)
Keywords: Chlorella vulgaris, Aluminum, copper toxicity, Antioxidant enzymes, Nitrate reductase,
Abstract :
Chlorella vulgaris beijernick was grown in medium BG-11 with 5 concentrations of copper (10, 20, 30, 40, 50μM) without aluminum or with aluminum (300μM) and pH 7.1 for 10 days in 20 hours light and 4 hours darkness. In this study, the effect of different treatments on the activity of peroxidase, catalase, Ascorbat peroxidase and superoxid dismotase and nitrate reductase, in vivo, was evaluated. Increase of copper concentration caused increase in activity of antioxidant enzymes. Catalase activity in the presence of aluminum in all copper treatments showed a significant reduction, but peroxidase and Ascorbat peroxidase activities in 20μM treatments decreased significantly when compared with control. The significant decrease in the activity of catalase, peroxidase and Ascorbat peroxidase activity, in 20μM copper with aluminum comparing with 20 μM copper without aluminum was also observed. Results also showed that the effect of different concentrations of copper without aluminum on nitrate reductase activity was not significant and most nitrate reductase activity in treatments with copper and aluminum was observed in 40μM that was significant when compared with 20μM treatment and was not significant when compared with other treatments or control. It is concluded that the presence of aluminum, by increasing some of antioxidant enzymes activities, lead to reduce of copper toxicity and algae growth increases.
ساطعی، آ.، و سروش نسب، ل. (1387). بررسی برخی از پاسخهای فیزیولوژی جلبک کلرلاولگاریس به اضافه کردن آلومینیوم در محیط کشت. پایان نامه کارشناسی ارشد. دانشگاه آزاد اسلامی واحد گرگان
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