Study of the effect of glutamic acid on some physiological properties of stevia (Stevia rebaudiana) under salinity stress using in vitro culture
Subject Areas : Environmental physiologyParastoo Majidian 1 * , Masoumeh Salemi 2 , Mahyar Gerami 3 , Masoud Azadbakht 4
1 - Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran
2 - Biology Department, Sana Institute of Higher Education, Sari, Iran
3 - Biology Department, Sana Institute of Higher Education, Sari, Iran
4 - Biology Department, Sana Institute of Higher Education, Sari, Iran
Keywords: antioxidant enzyme, Glutamic acid, Plant Tissue Culture, Salinity, Stevia,
Abstract :
The aim of this study was to evaluate the effect of salt stress and glutamic acid on some physiological traits in stevia in the condition of in vitro culture. This study was performed based on factorial completely randomized design with three replications at tissue culture laboratory of Pardis Univeristy of Tehran. The physiological parameters including photosynthetic pigments content, proline content, MDA content, and antioxidant enzyme activities were assessed according to interaction effect of glutamic acid and salinity. at last, the obtained data were calculated using Minitab 16 and SAS9.4 softwares. The results showed that stevia was apparently sensitive to 100 and 200 mM salinity concentrations. However, glutamic treatments had positive effects on physiological parameters against salinity stress. The accurate results indicated that 0.5 mg glutamic acid could neutralize the effect of 200 mM salinity. In addition, the obtained data exhibited that stevia could combat salinity side effects by increasing the activity of defective systems such as antioxidant enzyme like guaicol peroxidase and proline content.
Abdi, A. (2022). Assessment of drought stress on morphologic traits and latex of Russian dandelion. Master thesis, Tehran University.
Aghighi Shahverdi, M., Omidi, H. and Tabatabai, G. (2017). The effect of foliar Fe, Br and Selenium on morphologic traits of root and photosynthetic pigments of stevia under salt stress. Journal of Plant Environmental Physiology. 12(3): 1-18.
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology. 24(1): 1.
Ashraf, M. P. J. C. and Harris, P. J. C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science. 166(1): 3-16.
Bates, L.S., Waldren, R.A. and Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-207.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248–254
Corwin, D. L. (2021). Climate change impacts on soil salinity in agricultural areas. European Journal of Soil Science. 72(2): 842-862.
Franzoni, G., Cocetta, G., Trivellini, A., Garabello, C., Contartese, V. and Ferrante, A. (2022). Effect of exogenous application of salt stress and glutamic acid on lettuce (Lactuca sativa L.). Scientia Horticulturae. 299: 111027.
Gaspar, T., Wyndaele, R., Bouchet, M., and Ceulemans, E. (1977). Peroxidase and α‐amylase activities in relation to germination of dormant and nondormant wheat. Physiologia plantarum. 40(1): 11-14.
Gerami, M., Mohammadian, A, and Akbarpour, V. (2019). The effect of putrescine and salicylic acid on physiological characteristics and antioxidant in Stevia Rebaudiana B. under salinity stress. Journal of Crop Breeding. 11 (29): 40-54
Goharrizi, K. J., Meru, G., Kermani, S. G., Heidarinezhad, A., and Salehi, F. (2021). Short-term cold stress affects physiological and biochemical traits of pistachio rootstocks. South African Journal of Botany. 141: 90-98.
Habib Khodai, R. (2014). The effect of salt stress and glutamic acid on Khoshnav grape cultivar and evaluation the gene expression involved in resistant to salt stress. Master thesis, Tehran University.
Hasegawa, P. M., Bressan, R. A., Zhu, J. K. and Bohnert, H. J. (2000). Plant cellular and molecular responses to high salinity. Annual Review of Plant Biology. 51(1): 463-499.
Heath, R. L., and Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125: 189–198.
Helali, S. (2022). Assessment of glutamic acid on some morphological and biochemical properties of Rosmarinus officinalis under salt stress. Master thesis. Tehran University
Iqbal, P., Ghani, M.A., Ali, B., Shahid, M., Iqbal, Q., Ziaf, K. and Ahmad, J. (2021). Exogenous application of glutamic acid promotes cucumber (Cucumis sativus L.) growth under salt stress conditions. Emirates Journal of Food and Agriculture. 407-416.
Karimi, M., Ahmadi, A., Hashemi, J., Abbasi, A., Tavarini, S., Guglielminetti, L., and Angelini, L. G. (2015). The effect of soil moisture depletion on stevia (Stevia rebaudiana Bertoni) grown in greenhouse conditions: Growth, steviol glycosides content, soluble sugars and total antioxidant capacity. Scientia Horticulturae. 183: 93-99.
Liu, J., Fu, C., Li, G., Khan, M. N. and Wu, H. (2021). ROS homeostasis and plant salt tolerance: plant nanobiotechnology updates. Sustainability. 13(6): 3552.
Lück, H. (1965). Catalase. In Methods of enzymatic analysis (pp. 885-894). Academic press.
Neshat, M., Chavan, D. D., Shirmohammadi, E., Pourbabaee, A. A., Zamani, F., and Torkaman, Z. (2023). Canola inoculation with Pseudomonas baetica R27N3 under salt stress condition improved antioxidant defense and increased expression of salt resistance elements. Industrial Crops and Products. 206: 117648.
Rasouli Alamouti, M. (2015). The evaluation of salt stress on phytochemical yield and growth parameters of stevia under hydroponic condition. Master thesis, Payam Nor University.
Shah, S.H., Houborg, R. and McCabe, M.F. (2017). Response of chlorophyll, carotenoid and SPAD-502 measurement to salinity and nutrient stress in wheat (Triticum aestivum L.). Agronomy. 7(3): 61.
Singh, S. D. and Rao, G. P. (2005). Stevia: The herbal sugar of 21st century. Sugar Tech. 7(1): 17-24.
Sun, Y., Zhou, Q., Xie, X. and Liu, R. (2010). Spatial, sources and risk assessment of heavy metal contamination of urban soils in typical regions of Shenyang, China. Journal of Hazardous Materials. 174(1-3): 455-462.
Yan, N., Marschner, P., Cao, W., Zuo, C. and Qin, W. (2015). Influence of salinity and water content on soil microorganisms. International soil and water conservation Research. 3(4): 316-323.
You, M. K., Lee, Y. J., Kim, J. K., Baek, S. A., Jeon, Y. A., Lim, S. H., and Ha, S. H. (2020). The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds. BMC plant biology. 20: 1-16.
Zeng, J., Chen, A., Li, D., Yi, B., and Wu, W. (2013). Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry, 61(24), 5720-5726.