Study of the effect of auxin and gibberellin on growth, total chlorophyll, carotenoid and terpenoid content in Euphorbia trigona Mill.

Subject Areas : Environmental physiology

Hakimeh Rezaei ¹ , Aryan Sateei ² , Tahereh A. Aghajanzadeh ³ , Mehdi Ebadi ⁴

1 - Department of Plant Biology, Faculty of Basic Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2 - Department of Biology, Gorgan branch, Islamic Azad University, Gorgan, Iran,
3 - Department of Plant Sciences, Faculty of Science, University of Mazandaran, Babolsae, Iran
4 - Head of Chemical Analysis Research Center

Received: 2022-10-30 Accepted : 2022-12-25 Published : 2023-12-22

Keywords: Auxin, Carotenoid, Chlorophyll, Euphorbia trigona, GC-MS, Gibberellin, Indole butyric acid, Naphthalene acetic acid, Terpenoid,

Abstract :

This study was conducted to investigate the effect of auxin and gibberellin on Euphorbia trigona Mill. in a randomized complete block design in 2020, and in greenhouse conditions. One minute pretreatments including auxin regulators NAA, IBA and IAA at a concentration of 500 mg / l and treatments containing GA, IAA, IBA and NAA, 250 mg / l were compared with each other and with control plants. Stems and roots fresh weight, dry weight, dry weight percentage, total photosynthetic pigments content as well as total terpenoids were measured. Fresh and dry weight and the percentage of dry weight of stem did not show sensitivity to the treatments and pretreatments, and the effects were not significant. NAA treatment had the most positive effect on roots fresh and dry weight, although the percentage of root dry weight was not significantly affected by the treatments. The highest significant increase in terpenoid content was observed in NAA treatment and NAA and IBA pretreatments. GA treatment had the most positive effects on the content of each of chlorophylls a and b, total and carotenoid content. In terms of effect on photosynthetic antenna composition, the most positive effects on the ratio of total chlorophyll to carotenoids were observed in GA, NAA, IAA and IAA pretreatments. IAA treatment also showed a significant increase in chlorophyll a to b ratio. The correlation between growth parameters with each other and also the correlation of chlorophyll content with each other and with carotenoids was positive and significant. However, the correlation of terpenoid content with none of the other traits was significant. On the other hand and from an application point of view, based on gas chromatography-mass spectrometry measurements, naphthalene-acetic-acid treatment was effective in severely increasing the content of valuable medicinal compound, bis-2-ethylhexyl phthalate, compared to the control.

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