Molecular and biochemical protective roles of sodium nitroprusside in tomato (Lycopersicon esculentum Mill.) under salt stress

Subject Areas : Plant Physiology

Sahar Taheri ¹ , sakineh Saeidisar ² , Nahid Masoudian ³ , Mostafa Ebadi ⁴ , Bostan Roudi ⁵

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Agriculture, Faculty of Shariaty, Tehran Branch, Technical and Vocational
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
4 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
5 - Department of biology,faculty of science, Islamic Azad University, Damghan Branch ,Damghan,Iran

Received: 2020-10-16 Accepted : 2020-10-26 Published : 2020-10-01

Keywords: Salinity, Tomato, SNP, HKT1.2, SLWRKY 8,

Abstract :

Salinity stresses act as inhibitor factors of plant growth. They can change the physiological characteristics and limit the production of crops. Sodium nitroprusside (SNP) is a stable free radical which use as a signalling molecule in plants and participates in various plant’s physiological, biochemical and molecular processes and also in plant’s responses to environmental stresses. We investigated the effect of SNP on physiological parameters such as photosynthetic and non-enzymatic pigments, biochemical like APX and SOD enzymes and HKT1.2 and SLWRKY 8 genes expression as a molecular section on tomato under salt stress. In this study, SNP was used as nitric oxide (NO) donor. Tomato seedlings roots were subjected to various levels of salinity including 0, 40, 80 and 120 mM and SNP (0, 50 and 100 mM) for 20 days. The SNP had protective effects on photosynthetic parameters by increase in non-enzymatic and enzymatic antioxidants. It had also decrease and additive effect in HKT1.2 and SLWRKY 8 genes expression respectively.