Effects of eight weeks of interval training and casein supplementation on the fatty acid transporter FATCD 36 in obese women
Subject Areas : Biochemistry and Sport NutritionSomayeh Khosravanian 1 , abdolmajid emami 2 * , Samaneh Zare 3
1 - Master of Sports Nutrition, Department of Sports Sciences, Yazd Branch, Islamic Azad University, Yazd, Iran
2 - Assistant Professor, Department of Sport Sciences, Meybod Branch, Islamic Azad University, Meybod, Iran.
3 - Department of Sports Sciences, Payam Noor University, Yazd, Iran.
Keywords: Interval Training, Casein, Fatty Acid Transporter, Obesity.,
Abstract :
Many regulatory processes occur in fatty acid oxidation in skeletal muscle. For example, we can mention the transfer of this substrate into the muscle cell, because a large amount of the oxidized fatty acid is provided by plasma non-esterified fatty acids. Sex-related hormonal differences have a significant effect on substrate oxidation, such that the response of hormones affecting lipolysis, transport, and oxidation of fatty acids during exercise is different in women than in men. Interval training may have equal or greater metabolic benefits than other forms of exercise by increasing energy expenditure during and after activity and by shifting the substrate toward fat utilization. The limited research on the use of casein protein supplementation has introduced a new research paradigm related to the timing of protein intake. The aim of the present study was to determine and compare the effects of eight weeks of interval training and casein supplementation on the fatty acid transporter FATCD 36 in obese women. The statistical sample of this semi-experimental and applied study included 40 obese female volunteers who were divided by simple randomization into four equal groups: exercise, exercise and casein supplementation, casein supplementation, and control. The training groups performed a running interval training program for eight weeks, three sessions per week. The subjects ran a distance of 20 meters in 30 seconds at maximum speed back and forth, followed by 30 seconds of walking. The training in the first week consisted of four repetitions of activity and four rest periods, with one activity and one rest period added each week in the following weeks. Each subject consumed 20 grams of Pegah Company casein supplement powder dissolved in 200 cc of water with their lunch daily. The levels of the fatty acid transporter FATCD 36 were measured using a kit from Zelbio, Germany. The results of two-way ANOVA and Tukey's post hoc test showed that there was a significant difference between the mean levels of the fatty acid transporter FATCD 36 in all groups compared to the control group. Therefore, both eight weeks of interval training, eight weeks of casein supplementation, and the combination of exercise and supplementation have an effect on the fatty acid transporter FATCD 36 in obese women.
[1] Abdullah Karim Al-Shammari, Z., Banaeifar, A.A., Zafari, A., Arshadi, S. (2024). The interaction of interval training and whey protein on the ratio of testosterone to cortisol. Journal of Physiology of Training and Sports Injuries, 1(1):39-46. [Persian].
[2] Barzegar, H., Akbarnejad, A., Soori, R., Mazaheri, Z., Shabkhiz, F., Vosadi, E., Ranjbar, K. (2018). The Effect of High Intensity Interval Training on the Muscle CTRP15 Gene Expression and Adipocyte Fatty Acid Transporters in Adult Male Wistar Rats. Sport Physiology, 10(37), 203-216. doi: 10.22089/spj.2018.1171. [Persian].
[3] Basami, M., Ahmadizad, S., Hatefi, S. (2017). The Effect of Upper and Lower Body Interval Exercise on Fat Metabolism in Obese Women. Journal of Sport Biosciences, 9(3), 383-398. doi: 10.22059/jsb.2018.64771. [Persian].
[4] Dela Cruz, J., Kahan, D. (2021). Pre-Sleep Casein Supplementation, Metabolism, and Appetite: A Systematic Review. Nutrients, 13, 1872.
[5] Kevin Tipton, Metabolism, 815 Market St., Galveston, TX 77550; E-mail: ktipton@utmb.edu. Submitted for publication March 2014. Accepted for publication July 2014.
[6] Martinez Galan, B.S., GioloDe Carvalho, F., Carvalho, S. C. S., Cunha Brandao, C.F., Morhy Terrazas, S.I., Abud, G.F., Meirelles, M.S.S., Sakagute, S., Ueta Ortiz, G., Marchini, J.S., and et al. (2021). Casein and Whey Protein inthe Breast Milk Ratio: Could ItPromote Protein MetabolismEnhancement in Physically Active Adults? Nutrients, 13, 2153.
[7] Middendorf, D., Bindrich, U., Siemer, C., Töpfl, S., Heinz, V. (2021). Affecting Casein Micelles by Pulsed Electrical Field (PEF) for Inclusion of Lipophilic Organic Compounds. Appl.Sci., 11, 4611.
[8] Ravaut, G., Légiot, A., Bergeron, K.F., Mounier, C. (2021). Monounsaturated Fatty Acids in Obesity-Related Inflammation. Int. J. Mol. Sci., 22, 330. https://doi.org/10.3390/ijms22010330.
[9] Sari-Sarraf, V., Amirsasan, R., Parnian Khajehdizaj, N. (2020). Effects of HIIT and MICT Combined with ICR on FM and LBM in Overweight Women. Journal of Applied Health Studies in Sport Physiology, 7(2), 20-28. doi: 10.22049/jassp.2020.26885.1319. [Persian].
[10] Zarifi, A., Rajabi, H., Hasan nia, S., Dehkhoda, M., Mirsoltani, B. (2015). The Effect of Low Volume High Intensity Interval Training on Sarcolemmal Content of Fatty Acid Transport Proteins (FAT/CD36 and FABPpm) in Young Men. RSMT. 13 (10) :17-33
URL: http://jsmt.khu.ac.ir/article-1-145-fa.html. [Persian].