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Manuscript ID : EJMP-2112-1667 (R1) Visit : 134 Page: 77 - 94

10.30495/ejmp.2022.1947044.1667

20.1001.1.23223235.1401.10.4.5.5

Article Type: Original Research

Phytochemical investigation of antimicrobial effects of Moringa oleifera Lam extract propagated through tissue culture

Subject Areas : Phytochemistry

Asieh Eshag zadeh 1 (Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran)
Mahboubeh Taherkhani 2 (Department of Chemistry, College of science, Takestan Branch, Islamic Azad University, Takestan, Iran)

Received: 2021-12-12 Accepted : 2022-04-07 Published : 2022-12-22

Keywords: tissue culture, Extraction, Quercetin, Anti bacterial, Moringa oleifera Lam,

Abstract :

Moringa oleifera Lam. is a tropical tree belonging to the family Moringaceae with 14 species. This study investigated the phytochemical and antimicrobial effects of M. oleifera extract propagated through tissue culture in Iran in the spring of 2016. The extract of plant obtained from tissue culture was extracted by maceration in a solvent, and purified quercetin as a natural compound was extracted through a column and thin layer chromatography, then the molecular structure of flavonoid was identified through Nuclear Magnetic Resonance spectroscopy and Infrared technique. To estimate the antibacterial activity, the total plant extract was obtained by Methanol, Chloroform, and Ethyl acetate and finally the Minimal Inhibitory Concentration (MIC) and the Minimal Bactericidal Concentration (MBC) were determined. The antimicrobial effect of this plant against four bacterial strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli was investigated by plate well method in a liquid medium. The maximal inhibitory concentration (20 mm) was obtained from the methanolic extract. Also, the best results of MIC and MBC were obtained from the effect of 40 mg/ml of methanolic extract on the E. coli strain. Therefore, it is concluded that the extract of M. oleifera plant is a rich source of antioxidant compounds such as flavonoids, and also its methanolic extract has significant antimicrobial properties.

References:

References

  1. Akintelu, S.A., Folorunso, A.S. and Oyebamiji, A.K. (2021). Phytochemical and antibacterial investigation of Moringa oleifera seed: experimental and computational approaches. Eclética Química Journal, 46(2): 17-25.
    2.
  2. Akya, A., Mojarab, M., Farshchian, M. and Ahmadi, K. (2014). The effect of stem bark extracts of Tamarix ramosissim shrub on Pseudomonas aeruginosa isolates. Scientific Journal of Kurdistan University of Medical Sciences, 19(4): 128-134.
    3.
  3. Amabye, T.G. and Tadesse, F.M. (2016). Phytochemical and antibacterial activity of Moringa Oleifera available in the market of mekelle. Journal of Analytical and Pharmaceutical Research, 2(1): 00011. DOI: 10.15406/japlr.2016.02.00011.
    4.
  4. Anwar, F., Latif, S., Ashraf, M. and Gilani, A.H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research, 21(1):17-25.
    5.


  1. Anzano, A., Ammar, M., Papaianni, M., Grauso, L., Sabbah, M., Capparelli, R. and Lanzotti, V. (2021). Moringa oleifera Lam.: a phytochemical and pharmacological overview. Horticulturae, 7, 409. https://doi.org/10.3390/horticulturae7100409.
    2.
  2. Asare, G.A., Gyan, B., Bugyei, K., Adjei, S., Mahama, R., Addo, P., Otu-Nyarko, L., Wiredu, E.K. and Nyarko, A. (2012). Toxicity potentials of the nutraceutical Moringa oleiferaat supra supplementation levels. Journal of Ethnopharmacology, 139(1): 265-272.
    3.
  3. Azizian Shermeh, O., Taherizadeh, M., Valizadeh, M. and Qasemi, A. (2018). Robial and antioxidant activities and determining phenolic and flavonoid contents of the extracts of five species from different families of the medicinal plants grown in Sistan and Baluchestan province. Journal of Fasa University of Medical Sciences, 7: 465-479.
    4.
  4. Bagheri, G., Martorell, M., Ramírez-Alarcón, K., Salehi, B. and Sharifi-Rad, J. (2020). phytochemical screening of Moringa oleifera leaf extracts and their antimicrobial activities. Cellular and Molecular Biology (Noisy-le-grand, France), 66(1): 20-26. PMID: 32359378.
    5.
  5. Balouiri, M., Sadiki, M. and Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2): 71-79.
    6.
  6. Barreto, M.B., Freitas, J.V.B.D., Silveira, E.R., Bezerra, A.M.E., Nunes, E.P. and Gramosa, N.V. (2009). Volatile and non-volatile chemical constituents of Moringa oleifera Lam., Moringaceae. Revista Brasileira de Farmacognosia, 19(4): 893-897.
    7.
  7. Bina, B., Shasavani, A., Asghare, G. and Hasanzade, A. (2007). Comparison of water turbidity removal efficiencies of Moringa oleifera seed extract and poly-aluminum chloride. Journal of Water and Wastewater, 18(1): 24-33.
    8.
  8. Biswas, D., Nandy, S., Mukherjee, A., Pandey, D.K. and Dey, A. (2020). Moringa oleifera Lam. and derived phytochemicals as promising antiviral agents: A review. South African Journal of Botany, 129: 272-282. https://doi.org/10.1016/j.sajb.2019.07.049.
    9.
  9. Daghaghele, S., Kiasat, A.R., safiodin ardebili, S.M., Mirzajani, R., (2022). Evaluation of different extraction methods of phytochemical and antioxidant compounds of Moringa oleifera leaf extract. Journal of food science and technology (FSCT), 18(121) :163-172.
    10.
  10. Daghaghele, S., Kiasat, A.R., Safieddin Ardebili,S.M., Mirzajani, R. (2021). Intensification of extraction of antioxidant compounds from Moringa Oleifera leaves Using ultrasound-assisted approach: BBD-RSM design. International Journal of Fruit Science, 21(1): 693-705.
    11.
  11. Dhakad, A.K., Ikram, M., Sharma, S., Khan, S., Pandey, V.V. and Singh, A. (2019). Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytotherapy research: PTR, 33(11): 2870-2903. https://doi.org/10.1002/ptr.6475.
    12.
  12. Farooq, B., Koul, B., Mahant, D. and Yadav, D. (2021). Phytochemical analyses, antioxidant and anticancer activities of ethanolic leaf extracts of Moringa oleifera Lam. varieties. Plants (Basel, Switzerland), 10(11), 2348. https://doi.org/10.3390/plants10112348.
    13.
  13. Fahey, J.W. (2005). Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal, 1: 1-33.
    14.
  14. Fernandes, Â., Bancessi, A., Pinela, J., Dias, M. I., Liberal, Â., Calhelha, R. C., Ćirić, A., Soković, M., Catarino, L., Ferreira, I. and Barros, L. (2021). Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). Food chemistry, 341(Pt 1): 128229. https://doi.org/10.1016/j.foodchem.2020.128229.
    15.
  15. Govahi, M., Ghorbani, F., Ranjbar, M., Rahaiee, S. and Azizi, H. (2019). Evaluation of antioxidant and antibacterial activity and determination of phenolic and flavonoid content of aqueous and methanolic extracts of Scutellaria pekinensis. Journal of Ilam University of Medical Sciences, 27(3): 91-100.
    16.
  16. Gull, I., Javed, A., Aslam, M.S., Mushtaq, R. and Athar, M.A. (2016). Use of Moringa oleifera flower pod extract as natural preservative and development of SCAR marker for Its DNA based identification. Bio Med research international, 2016: 7584318. https://doi.org/10.1155/2016/7584318.
    17.
  17. Hendra, R., Ahmad, S., Sukari, A., Shukor, M.Y. and Oskoueian, E. (2011). Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff) boerl. fruit. International Journal of Molecular Sciences, 12(6): 3422-3431.
    18.
  18. Katayon, S., Megat Mohd Noor, M.J., Asma, M., Abdul Ghani, L.A., Thamer, A.M., Azni, I., Ahmad, J. Khor, B.C., and Suleyman, A.M. (2006). Effects of storage condition of Moringa oleifera seeds on its performance in coagulation. Bioresource Technology, 97(13): 1455-1460.
    19.
  19. Kebreab, A.G. (2004). Moringa seed and pumice as alternative natural materials for drinking water treatment. KTH Land and Water Resources Engineering Univ., Trital wr PhD Thesis 1013.
    20.
  20. Khorasani Esmaeili, A., Taha, R.M., Mohajer, S. and Banisalam, B. (2015). Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pretense L. (Red clover). Bio Med Research International, 2015: 1-11.
    21.
  21. Khoroushi M, Khorasgani M R, Aliasghari A. (2017). Determination of minimum inhibitory concentration (MIC) of two plants extract on cariogenic streptococci. Journal of Dental Medicine, 30(1) :12-17.
    22.
  22. Korkina, L.G. and Afanas'ev, I.B. (1996). Antioxidant and chelating properties of flavonoids. Adv Pharmacol 38: 151-163.
    23.
  23. Landázuri, A.C., Gualle, A., Castañeda, V., Morales, E., Caicedo, A. and Orejuela-Escobar, L. M. (2020). Moringa oleifera Lam. leaf powder antioxidant activity and cytotoxicity in human primary fibroblasts. Natural Product Research, DOI: 10.1080/14786419.2020.1837804.
    24.
  24. Lenin, S., Ramasamy, S. and Revathy, D. (2021). Evaluate the effectiveness of phytochemical, physicochemical and mineral analysis of Moringa oleifera (Drum Stick Leaves). Indian Journal of Pharmacy & Drugs Studies, 2(2): 20-24.https://mansapublishers.com/IJPDS/article/view/2959
    25.
  25. Marrufo, T., Nazzaro, F., Mancini, E., Fratianni, F., Coppola, R., De Martino, L., Bela Agostinho, A. and De Feo, V. (2013). Chemical composition and biologicala of the essential oil from leaves of Moringa oleifera Lam. Cultivated in Mozambique. Molecules, 18(9): 10989-11000.
    26.
  26. Meireles, D., Gomes, J., Lopes, L., Hinzmann, M. and Machado, J. (2020). A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine, 20: 495-515. https://doi.org/10.1007/s13596-020-00468-0.
    27.
  27.  Minaiyan, M., Asghari, G., Taheri, D., Saeidi, M. and Nasr-Esfahani, S. (2014). Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats. Avicenna Journal of Phytomedicine, 4(2): 127-136.
    28.


  1. Moeini, F., Mohammadi-Sichani, M. and Shahanipoor. K. (2015). Evaluation of the antibacterial effect of methanol and aqueous extracts of Vaccinium Arctostaphylos fruit against Salmonella spp in vitro. Journal of Rafsanjan University of Medical Sciences,14(4): 257-268.
    2.
  2. Mukunzi, D., Nsor-Atindana, J., Xiaoming, Z., Gahungu, A., Karangwa, E. and Mukamurezi, G. (2011). Comparison of volatile profile of Moringa oleifera leaves from Rwanda and China using HS-SPME. Pakistan Journal of Nutrition, 10(7): 602-608.
    3.
  3. Mumtaz, M.Z., Kausar, F., Hassan, M., Javaid, S. and Malik , A. (2021). Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study. Beni-Suef University Journal of Basic and Applied Sciences, 10(12): 1-11.
    4.
  4. Nizioł-Łukaszewska, Z., Furman-Toczek, D., Bujak, T., Wasilewski, T. and Hordyjewicz-Baran, Z. (2020). Moringa oleifera L. extracts as bioactive ingredients that increase safety of body wash cosmetics. Dermatology Research and Practice, Article ID 8197902, 14 pages, https://doi.org/10.1155/2020/8197902.
    5.
  5. Rashidi, Z., Khosravizadeh, Z., Talebi, A., Khodamoradi, K., Ebrahimi, R. and Amidi, F. (2021). Overview of biological effects of quercetin on ovary. Phytotherapy Research, 35(1): 33-49.
    6.
  6. Reyes-Becerril, M., Angulo, C. and Silva-Jara, J. (2021). Antibacterial and immunomodulatory activity of Moringa (Moringa oleifera) seed extract in Longfin yellowtail (Seriola rivoliana) peripheral blood leukocytes. Aquaculture Research, 52(9): 4076-4085.
    7.
  7. Shah, K.H. and Oza, M.J. (2020). Comprehensive review of bioactive and molecular aspects of Moringa oleifera Lam. Food reviews international, https://doi.org/10.1080/87559129.2020.1813755.
    8.
  8. Sharma, P., Wichaphon, J. and Klangpetch, W. (2020). Antimicrobial and antioxidant activities of defatted Moringa oleifera seed meal extract obtained by ultrasound-assisted extraction and application as a natural antimicrobial coating for raw chicken sausages. International Journal of Food Microbiology, 332(2): 2020, 108770.
    9.
  9. Sultana, S. (2020). Nutritional and functional properties of Moringa oleifera. Metabolism Open, 8: 100061. https://doi.org/10.1016/j.metop.2020.100061
    10.
  10. Taherkhani, M. and Rustaiyan, A. (2016). Investigation of in vitro cytotoxic, mutagenic and anti-mutagenic effects of shirazolide extracted from Jurinea leptoloba. Natural Product Research, 17: 1-4.
    11.
  11. Wallace, J.G.S., Kumar, S.N., Smila, V.N., Nivitha, T., Nithaniyal, S., Satapathy, S., Acharya, K. and Mondal, E. (2021). Validation of tribal claims on Moringa oleifera lam. using phytochemical analysis. Plant Archives, 21(1): 1838-1841.
    12.
  12. Xiao, X., Wang, J., Meng, C., Liang, W., Wang, T., Zhou, B., Wang, Y., Luo, X., Gao, L. and Zhang, L. (2020). Moringa oleifera Lam and its therapeutic effects in immune disorders. Frontiers in pharmacology, 11: 566783. https://doi.org/10.3389/fphar.2020.566783.
    13.
  13. Xie, J., Peng, L. J., Yang, M. R., Jiang, W. W., Mao, J. Y., Shi, C. Y., Tian, Y. and Sheng, J. (2021). Alkaloid extract of Moringa oleifera Lam. exerts antitumor activity in human non-small-cell lung cancer via modulation of the JAK2/STAT3 signaling pathway. Evidence-based complementary and alternative medicine, Article ID: 5591687. https://doi.org/10.1155/2021/5591687.
    14.
  14. Xu, Y., Chen, G. and Guo, M. (2021).Correlations between phytochemical fingerprints of Moringa oleifera leaf extracts and their antioxidant activities revealed by chemometric analysis. Phytochemical analysis, 32(5): 698-709.
    15.


  1. Yi, H., Peng, H., Wu, X., Xu,X., Kuang, T., Zhang, J., Du, L. and Fan, G. (2021). The therapeutic effects and mechanisms of quercetin on metabolic diseases: pharmacological data and clinical evidence. Oxidative Medicine and Cellular Longevity, https://doi.org/10.1155/2021/6678662.
    2.
  2. Zhang, Y., Peng, L., Li, W., Dai, T., Nie, L., Xie, J., Ai, Y., Li, L., Tian, Y. and Sheng, J. (2020). Polyphenol extract of Moringa oleifera leaves alleviates colonic inflammation in dextran Sulfate Sodium-Treated Mice. Evidence-based complementary and alternative medicine, Article ID: 6295402. https://doi.org/10.1155/2020/6295402.
    3.
_||_

References

  1. Akintelu, S.A., Folorunso, A.S. and Oyebamiji, A.K. (2021). Phytochemical and antibacterial investigation of Moringa oleifera seed: experimental and computational approaches. Eclética Química Journal, 46(2): 17-25.
    2.
  2. Akya, A., Mojarab, M., Farshchian, M. and Ahmadi, K. (2014). The effect of stem bark extracts of Tamarix ramosissim shrub on Pseudomonas aeruginosa isolates. Scientific Journal of Kurdistan University of Medical Sciences, 19(4): 128-134.
    3.
  3. Amabye, T.G. and Tadesse, F.M. (2016). Phytochemical and antibacterial activity of Moringa Oleifera available in the market of mekelle. Journal of Analytical and Pharmaceutical Research, 2(1): 00011. DOI: 10.15406/japlr.2016.02.00011.
    4.
  4. Anwar, F., Latif, S., Ashraf, M. and Gilani, A.H. (2007). Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research, 21(1):17-25.
    5.


  1. Anzano, A., Ammar, M., Papaianni, M., Grauso, L., Sabbah, M., Capparelli, R. and Lanzotti, V. (2021). Moringa oleifera Lam.: a phytochemical and pharmacological overview. Horticulturae, 7, 409. https://doi.org/10.3390/horticulturae7100409.
    2.
  2. Asare, G.A., Gyan, B., Bugyei, K., Adjei, S., Mahama, R., Addo, P., Otu-Nyarko, L., Wiredu, E.K. and Nyarko, A. (2012). Toxicity potentials of the nutraceutical Moringa oleiferaat supra supplementation levels. Journal of Ethnopharmacology, 139(1): 265-272.
    3.
  3. Azizian Shermeh, O., Taherizadeh, M., Valizadeh, M. and Qasemi, A. (2018). Robial and antioxidant activities and determining phenolic and flavonoid contents of the extracts of five species from different families of the medicinal plants grown in Sistan and Baluchestan province. Journal of Fasa University of Medical Sciences, 7: 465-479.
    4.
  4. Bagheri, G., Martorell, M., Ramírez-Alarcón, K., Salehi, B. and Sharifi-Rad, J. (2020). phytochemical screening of Moringa oleifera leaf extracts and their antimicrobial activities. Cellular and Molecular Biology (Noisy-le-grand, France), 66(1): 20-26. PMID: 32359378.
    5.
  5. Balouiri, M., Sadiki, M. and Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2): 71-79.
    6.
  6. Barreto, M.B., Freitas, J.V.B.D., Silveira, E.R., Bezerra, A.M.E., Nunes, E.P. and Gramosa, N.V. (2009). Volatile and non-volatile chemical constituents of Moringa oleifera Lam., Moringaceae. Revista Brasileira de Farmacognosia, 19(4): 893-897.
    7.
  7. Bina, B., Shasavani, A., Asghare, G. and Hasanzade, A. (2007). Comparison of water turbidity removal efficiencies of Moringa oleifera seed extract and poly-aluminum chloride. Journal of Water and Wastewater, 18(1): 24-33.
    8.
  8. Biswas, D., Nandy, S., Mukherjee, A., Pandey, D.K. and Dey, A. (2020). Moringa oleifera Lam. and derived phytochemicals as promising antiviral agents: A review. South African Journal of Botany, 129: 272-282. https://doi.org/10.1016/j.sajb.2019.07.049.
    9.
  9. Daghaghele, S., Kiasat, A.R., safiodin ardebili, S.M., Mirzajani, R., (2022). Evaluation of different extraction methods of phytochemical and antioxidant compounds of Moringa oleifera leaf extract. Journal of food science and technology (FSCT), 18(121) :163-172.
    10.
  10. Daghaghele, S., Kiasat, A.R., Safieddin Ardebili,S.M., Mirzajani, R. (2021). Intensification of extraction of antioxidant compounds from Moringa Oleifera leaves Using ultrasound-assisted approach: BBD-RSM design. International Journal of Fruit Science, 21(1): 693-705.
    11.
  11. Dhakad, A.K., Ikram, M., Sharma, S., Khan, S., Pandey, V.V. and Singh, A. (2019). Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytotherapy research: PTR, 33(11): 2870-2903. https://doi.org/10.1002/ptr.6475.
    12.
  12. Farooq, B., Koul, B., Mahant, D. and Yadav, D. (2021). Phytochemical analyses, antioxidant and anticancer activities of ethanolic leaf extracts of Moringa oleifera Lam. varieties. Plants (Basel, Switzerland), 10(11), 2348. https://doi.org/10.3390/plants10112348.
    13.
  13. Fahey, J.W. (2005). Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal, 1: 1-33.
    14.
  14. Fernandes, Â., Bancessi, A., Pinela, J., Dias, M. I., Liberal, Â., Calhelha, R. C., Ćirić, A., Soković, M., Catarino, L., Ferreira, I. and Barros, L. (2021). Nutritional and phytochemical profiles and biological activities of Moringa oleifera Lam. edible parts from Guinea-Bissau (West Africa). Food chemistry, 341(Pt 1): 128229. https://doi.org/10.1016/j.foodchem.2020.128229.
    15.
  15. Govahi, M., Ghorbani, F., Ranjbar, M., Rahaiee, S. and Azizi, H. (2019). Evaluation of antioxidant and antibacterial activity and determination of phenolic and flavonoid content of aqueous and methanolic extracts of Scutellaria pekinensis. Journal of Ilam University of Medical Sciences, 27(3): 91-100.
    16.
  16. Gull, I., Javed, A., Aslam, M.S., Mushtaq, R. and Athar, M.A. (2016). Use of Moringa oleifera flower pod extract as natural preservative and development of SCAR marker for Its DNA based identification. Bio Med research international, 2016: 7584318. https://doi.org/10.1155/2016/7584318.
    17.
  17. Hendra, R., Ahmad, S., Sukari, A., Shukor, M.Y. and Oskoueian, E. (2011). Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff) boerl. fruit. International Journal of Molecular Sciences, 12(6): 3422-3431.
    18.
  18. Katayon, S., Megat Mohd Noor, M.J., Asma, M., Abdul Ghani, L.A., Thamer, A.M., Azni, I., Ahmad, J. Khor, B.C., and Suleyman, A.M. (2006). Effects of storage condition of Moringa oleifera seeds on its performance in coagulation. Bioresource Technology, 97(13): 1455-1460.
    19.
  19. Kebreab, A.G. (2004). Moringa seed and pumice as alternative natural materials for drinking water treatment. KTH Land and Water Resources Engineering Univ., Trital wr PhD Thesis 1013.
    20.
  20. Khorasani Esmaeili, A., Taha, R.M., Mohajer, S. and Banisalam, B. (2015). Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pretense L. (Red clover). Bio Med Research International, 2015: 1-11.
    21.
  21. Khoroushi M, Khorasgani M R, Aliasghari A. (2017). Determination of minimum inhibitory concentration (MIC) of two plants extract on cariogenic streptococci. Journal of Dental Medicine, 30(1) :12-17.
    22.
  22. Korkina, L.G. and Afanas'ev, I.B. (1996). Antioxidant and chelating properties of flavonoids. Adv Pharmacol 38: 151-163.
    23.
  23. Landázuri, A.C., Gualle, A., Castañeda, V., Morales, E., Caicedo, A. and Orejuela-Escobar, L. M. (2020). Moringa oleifera Lam. leaf powder antioxidant activity and cytotoxicity in human primary fibroblasts. Natural Product Research, DOI: 10.1080/14786419.2020.1837804.
    24.
  24. Lenin, S., Ramasamy, S. and Revathy, D. (2021). Evaluate the effectiveness of phytochemical, physicochemical and mineral analysis of Moringa oleifera (Drum Stick Leaves). Indian Journal of Pharmacy & Drugs Studies, 2(2): 20-24.https://mansapublishers.com/IJPDS/article/view/2959
    25.
  25. Marrufo, T., Nazzaro, F., Mancini, E., Fratianni, F., Coppola, R., De Martino, L., Bela Agostinho, A. and De Feo, V. (2013). Chemical composition and biologicala of the essential oil from leaves of Moringa oleifera Lam. Cultivated in Mozambique. Molecules, 18(9): 10989-11000.
    26.
  26. Meireles, D., Gomes, J., Lopes, L., Hinzmann, M. and Machado, J. (2020). A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine, 20: 495-515. https://doi.org/10.1007/s13596-020-00468-0.
    27.
  27.  Minaiyan, M., Asghari, G., Taheri, D., Saeidi, M. and Nasr-Esfahani, S. (2014). Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats. Avicenna Journal of Phytomedicine, 4(2): 127-136.
    28.


  1. Moeini, F., Mohammadi-Sichani, M. and Shahanipoor. K. (2015). Evaluation of the antibacterial effect of methanol and aqueous extracts of Vaccinium Arctostaphylos fruit against Salmonella spp in vitro. Journal of Rafsanjan University of Medical Sciences,14(4): 257-268.
    2.
  2. Mukunzi, D., Nsor-Atindana, J., Xiaoming, Z., Gahungu, A., Karangwa, E. and Mukamurezi, G. (2011). Comparison of volatile profile of Moringa oleifera leaves from Rwanda and China using HS-SPME. Pakistan Journal of Nutrition, 10(7): 602-608.
    3.
  3. Mumtaz, M.Z., Kausar, F., Hassan, M., Javaid, S. and Malik , A. (2021). Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study. Beni-Suef University Journal of Basic and Applied Sciences, 10(12): 1-11.
    4.
  4. Nizioł-Łukaszewska, Z., Furman-Toczek, D., Bujak, T., Wasilewski, T. and Hordyjewicz-Baran, Z. (2020). Moringa oleifera L. extracts as bioactive ingredients that increase safety of body wash cosmetics. Dermatology Research and Practice, Article ID 8197902, 14 pages, https://doi.org/10.1155/2020/8197902.
    5.
  5. Rashidi, Z., Khosravizadeh, Z., Talebi, A., Khodamoradi, K., Ebrahimi, R. and Amidi, F. (2021). Overview of biological effects of quercetin on ovary. Phytotherapy Research, 35(1): 33-49.
    6.
  6. Reyes-Becerril, M., Angulo, C. and Silva-Jara, J. (2021). Antibacterial and immunomodulatory activity of Moringa (Moringa oleifera) seed extract in Longfin yellowtail (Seriola rivoliana) peripheral blood leukocytes. Aquaculture Research, 52(9): 4076-4085.
    7.
  7. Shah, K.H. and Oza, M.J. (2020). Comprehensive review of bioactive and molecular aspects of Moringa oleifera Lam. Food reviews international, https://doi.org/10.1080/87559129.2020.1813755.
    8.
  8. Sharma, P., Wichaphon, J. and Klangpetch, W. (2020). Antimicrobial and antioxidant activities of defatted Moringa oleifera seed meal extract obtained by ultrasound-assisted extraction and application as a natural antimicrobial coating for raw chicken sausages. International Journal of Food Microbiology, 332(2): 2020, 108770.
    9.
  9. Sultana, S. (2020). Nutritional and functional properties of Moringa oleifera. Metabolism Open, 8: 100061. https://doi.org/10.1016/j.metop.2020.100061
    10.
  10. Taherkhani, M. and Rustaiyan, A. (2016). Investigation of in vitro cytotoxic, mutagenic and anti-mutagenic effects of shirazolide extracted from Jurinea leptoloba. Natural Product Research, 17: 1-4.
    11.
  11. Wallace, J.G.S., Kumar, S.N., Smila, V.N., Nivitha, T., Nithaniyal, S., Satapathy, S., Acharya, K. and Mondal, E. (2021). Validation of tribal claims on Moringa oleifera lam. using phytochemical analysis. Plant Archives, 21(1): 1838-1841.
    12.
  12. Xiao, X., Wang, J., Meng, C., Liang, W., Wang, T., Zhou, B., Wang, Y., Luo, X., Gao, L. and Zhang, L. (2020). Moringa oleifera Lam and its therapeutic effects in immune disorders. Frontiers in pharmacology, 11: 566783. https://doi.org/10.3389/fphar.2020.566783.
    13.
  13. Xie, J., Peng, L. J., Yang, M. R., Jiang, W. W., Mao, J. Y., Shi, C. Y., Tian, Y. and Sheng, J. (2021). Alkaloid extract of Moringa oleifera Lam. exerts antitumor activity in human non-small-cell lung cancer via modulation of the JAK2/STAT3 signaling pathway. Evidence-based complementary and alternative medicine, Article ID: 5591687. https://doi.org/10.1155/2021/5591687.
    14.
  14. Xu, Y., Chen, G. and Guo, M. (2021).Correlations between phytochemical fingerprints of Moringa oleifera leaf extracts and their antioxidant activities revealed by chemometric analysis. Phytochemical analysis, 32(5): 698-709.
    15.


  1. Yi, H., Peng, H., Wu, X., Xu,X., Kuang, T., Zhang, J., Du, L. and Fan, G. (2021). The therapeutic effects and mechanisms of quercetin on metabolic diseases: pharmacological data and clinical evidence. Oxidative Medicine and Cellular Longevity, https://doi.org/10.1155/2021/6678662.
    2.
  2. Zhang, Y., Peng, L., Li, W., Dai, T., Nie, L., Xie, J., Ai, Y., Li, L., Tian, Y. and Sheng, J. (2020). Polyphenol extract of Moringa oleifera leaves alleviates colonic inflammation in dextran Sulfate Sodium-Treated Mice. Evidence-based complementary and alternative medicine, Article ID: 6295402. https://doi.org/10.1155/2020/6295402.
    3.

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