Phytochemical Study of Ethanolic Extract of Fraxinus excelsior L. in Different Habitats of Hyrcanian Forests in Mazandaran Province
Subject Areas : PhytochemistryMasoomeh Soleimany Rahim abadi 1 , seyed mohammad hosseini nar 2 , hamid jalilvand 3 , seyed mohammad hojatti 4 , poriya biparva 5
1 - Ph.D. Student, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources
University, Sari, Iran
2 - Associate Prof., Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources
University, Sari, Iran
3 - Professor, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University,
Sari, Iran
4 - Associate Prof., Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources
University, Sari, Iran
5 - Decomposition Chemistry Group, Sari Agricultural Sciences and Natural Resources University,
Sari, Iran
Keywords: Carvacrol, Mazandaran Province, Skin extract, Scopoltin, Key words: Fraxinus excelsior L,
Abstract :
Fraxinus excelsior L. is a forest species with versatile biological and medicinal activities that its extracts are used as a model for making new compounds in the treatment of various human diseases. In this study, in order to investigate how the biochemical characteristics of this forest species are affected by environmental conditions, four habitats of Perchink, Amreh, Alandan and Qarnsara with an altitude of 200 to 1400 meters, were selected in Tajan and Siahroud watersheds located in Mazandaran province. In each habitat, skin samples were taken from 3 trees (repeated) in summer. Ethanol extracts were obtained by maceration and were analyzed by GC/MS and the physical and chemical properties of the soil samples were measured. The most important compounds of extracts were included: benzeneethanol (30.33-51.35%), d-allose (6.20-31.72%), scopoletin (14.65-26.83%), tyrosol acetate (5.37-9.06%), carvacrol (0-4.93%), homovanillyl alcohol (3.84- 6.53%) which increased with increasing altitude. Also, there was negative relationship between the identified compounds and soil nutrients. Among them, only polyphenol tyrosol acetate behaved differently from the other compounds in a way that showed a negative relationship with the altitude factor and a positive correlation with the amount of carbon and absorbable phosphorus in the soil. Also, the chemical composition of d-allose was not observed in Perchink habitat. These results were showed that the chemical properties of this tree skin are affected by habitat conditions, which caused increases the species' resistance to pathogens. As a result, considering that the amount of active ingredients in this species was higher at higher altitudes and the presence of these compounds increases the resistance to pathogens, it seems planting this species is more productive at higher altitudes to achieve multiple goals in afforestation.
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Mehri-Rad, N., Payamenoor, V., and Nazari, J. 2016. Effect of base age and light on callus production of Betula litwinowii and induced botulin in vitro. Journal of genetic research and breeding of range and forest plants of Iran, 23:93-102. (In Persian).
Munoz, F., Marçais, B., Dufour, J., and Dowkiw, A. 2016. Rising out of the ashes: additive genetic variation for crown and collar resistance to Hymenoscyphus fraxineus in Fraxinus
excelsior. Journal of Phytopathology, 106:1535-1543.
Noguchi, C., Kamitori, K., Hossain, A., Hiroshi, H., Ayako, K., Youyi, D., Li S., Tokuda, M. and Fuminori, Y. 2016. DAllose inhibits cancer cell growth byreducing gLUT1 expression. The TohokuJournal of Experimental Medicine, 238: 131-141.
Naghdi Badi, H., Abdollahi, M., Mehrafarin, A., Ghorbanpour, M., Toylat, M., Gaderi, A. and Ghiaci, Y. 2017. An overview on two valuable natural and bioactive compounds, thymol and carvacrol, in medicinal plants. Journal of Medicinal Plants, 16(63): 1-32
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Oussalah, M., Caillet, S., Saucier, L. and Lacroix, M. 2007. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E-coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Journal of Food Control, 18 (5):414-420.
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excelsior. Journal of Phytopathology, 106:1535-1543.
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excelsior. Journal of Phytopathology, 106:1535-1543.