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  • Phytochemical evaluation and antioxidant activity of methanolic extracts of Momordica charantia L., Cucurbita pepo L. cultivar pumpkin, Ecballium elaterium (L.) A.Rich, Lagenaria siceraria L. cultivar Marankka

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Manuscript ID : EJMP-1805-1355 (R6) Visit : 315 Page: 26 - 39

20.1001.1.23223235.1397.6.4.3.5

Article Type: Original Research

Phytochemical evaluation and antioxidant activity of methanolic extracts of Momordica charantia L., Cucurbita pepo L. cultivar pumpkin, Ecballium elaterium (L.) A.Rich, Lagenaria siceraria L. cultivar Marankka

Subject Areas : Phytochemistry

zaynab mohkami 1 , عزیزاله خیری 2 , mahdi tavakolizadeh esfahani 3 , Mohsen Sani Khani 4 , abas bahari 5

1 - Phd Student, Dep.t of Horticulture, Faculty of Agriculture, University of Zanjan, Iran.
2 - عضو هیئت علمی دانشگاه زنجان
3 - 3Assist. Prof., Pharmacy of School, Zanjan University ofsciences medical , Iran.
4 - Department of Horticulture, Faculty of Agriculture, University of Zanjan, Iran
5 - Institute of Biotechnology, University of Zanjan, Iran

Received: 2018-05-13 Accepted : 2019-02-03 Published : 2019-02-20

Keywords: Pumpkin, antioxidant activity, Ecballium, Karella, Maranka,

Abstract :

ABSTRACT This study was designed to investigate the total phenolic content, total flavonoid content and antioxidant activity of fruits methanolic extracts from Momordica charantia L., Ecballium elaterium (L.) A.Rich, Cucurbita pepo L. cultivar pumpkin, Lagenaria siceraria L. cultivar Marankka belonging to Cucurbitaceae family. For this purpose, a factorial experiment was conducted in a completely randomized design with three replications. The matured fruits were harvested from the research farm in Zanjan university during the September to December 2017. Phytochemical characteristics of total phenol content (Folin–‌Ciocalteu method), total flavonoid (Aluminum Chloride method) and antioxidant activity (DPPH method) were evaluated. The highest level of phenolic and flavonoid contents was recorded for the fruit methanolic extracts from the Ecballium elaterium (L.) and Momordica charantia L. , with 85/5 ± 3/5 mg GAE/g and 12.13 ± 0.8, respectively. The results of DPPH test showed that the methanolic extract of Momordica charantia exhibited potent activity (97.09 % ± 0.96), followed by Ecballium elaterium (87.8% ± 1.5) and the lowest antioxidant activity related to methanolic extract of pumpkin fruit (58/02 % ± 2.7). Furthermore, there was a strong correlation between flavonoids content and antioxidant activity (P = 0.87) and also there was a positive correlation between phenolic content and antioxidant activity (P = 0.54). Generally, the phytochemical analysis showed that, Ecballium and Karella extracts are rich in polyphenol compounds against other sspecies and can be used as food.drug-

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References

  1. Attard, E. and Attard, H., 2008. Antitryosin activity of extracts from Ecballiumelaterium seeds. Fitoterapia, 79(3): 226-228.
    2.
  2. Attard, E., Brincat, M.P. and Cuschieri, A., 2005. Immunomodulatory activity of cucurbitacin E isolated from Ecballiumelaterium.Fitoterapia, 76(5): 439-441.
    3.
  3. Bahorun, T., Grinier, B., Trotin, F., Brunet, G., Pin, T., Luncky, M., Vasseur, J., Cazin, M., Cazin, C. and Pinkas, M., 1996. Oxygen species scavenging activity of phenolic extracts from Hawthorn fresh plant organs and pharmaceuticalpreparations. Arzneimittel-Forschung, 46(11): 1086-1089.
    4.
  4. Barros, L., Baptista, P. and Ferreira, I.C.F.R., 2007. Effect of fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Lactarius piperatus .Food and Chemical Toxicology, 45(9): 1731-1737. 
    5.
  5. Beloina, N., Gbeassorb, M., Akpaganab, K., Hudsonc, J., Soussab,K., Koumaglob, K. and Arnasona, J.T., 2005. Ethnomedicinal uses of Momordicacharantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. Journal of Ethnopharmacology, 96: 49-55.
    6.
  6. Bernard, S.A., and Olayinka, O.A,.2010. Search for a novel antioxidant, anti-inflammatory/analgesic or anti-proliferative drug: Cucurbitacins hold the ace. Journal of Medicinal Plants Research, 4(25): 2821-2826.
    7.
  7. Bizid, S., Sabbah, M., Msakni, I., Ben Slimene, B., Mohamed, G., Bouali, R., Ben Abdallah, H. and Abdelli, N., 2014. Cholestatic hepatitis due to Ecballiumelaterium. IngestionClinics and Research in Hepatology and Gastroenterology, 39(5): 61-63.
    8.
  8. Bohlooli, S., Jafari, N. and Jahed, S., 2012. Cytotoxic effect of freeze-dried extract of Ecballiumelateriumfruit on gastric adenocarcinoma (AGS) and esophageal squamous cell carcinoma (KYSE30) cell lines. Journal of Gastrointestinal Cancer, 43(4): 579-583.
    9.
  9. Carvalho, L.M.J., Smiderle, L., Carvalho J.L.V., Cardoso F.S.N. and Koblitz M.G.B.,2014. Assessment of carotenoids in pumpkins after different home cookingconditions. Food Science and Technology, 34:76-77.
    10.
  10. Chang, S.T., Wu, J.H., Wang, S.Y., Kang, P.L., Yang, N.S and Shyur, L.F., 2001. Antioxidant activity of extracts from Acacia confusa bark and heartwood. Journal of Agricultural & Food Chemistry, 49(7): 3420–3424.
    11.
  11. Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3): 178-182.
    12.
  12. Chirag, P., Tyagi, S.,Halligudi, N., Yadav, J., Pathak, S.,  Singh, S., Pandey, A.,  Singh, D. and Shankar K., 2013. Antioxidant activity of herbal plants: a recent review. Journal of Drug Discovery and Therapeutics, 1(8): 1-8.
    13.
  13. Grover, J.K. and Yadav, S.P., 2004. Pharmacological actions and potential uses ofMomordicacharantia: a review. Journal of Ethnopharmacology, 93: 123–132.
    14.
  14. Diaz, P., Jeong, S.C., Lee, S., Khoo, C. and Koyyalamudi, S.R., 2012. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese Medicine, 7(1): 1-9.
    15.
  15. Dou, D., Leng, P., Li, Y., Zeng, Y. and Sun, Y., 2013. Comparative study of antioxidant compounds and antiradical properties of the fruit extracts from three varieties of Crataeguspinnatifida.Journal of Food Science and Technology, 52(1): 430-436.
    16.
  16. Egea, I., Sanchez-Bel, P., Romojaro, F. and Pretel, M. T., 2010. Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods for Human Nutrition, 65(2): 121-129.
    17.
  17. El Naggar, M.B., Chalupova, M., Prazanova, G., Parak, T., Švajdlenka, E., Žemlicka, M. and Suchy, P., 2015. Hepatoprotective and proapoptotic effect of Ecballiumelaterium on CCl-induced hepatotoxicity in rats. Asian Pacific Journal of Tropical Medicine, 8(7): 526-531. 
    18.
  18. Felhi, S., Hajlaoui, H., Ncir, M., Bakari, S., Ktari, N., Saoudi, M., Gharsallah, N. and Kadri, A., 2016.Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities. Food Science and Technology, 36(4): 646-655.
    19.
  19. Firouzkoohi, F., Esmaeilzadeh Bahabadi, S., Mohkami, Z. and Yosefzaei, F., 2018.The effect of different solvents on total phenolic, flavonoid contents and antioxidant activity of different organs of Momordicacharantia L. cultured in Sistan region.Journal of medicinal plants ecophytochemistry, 5(4): 74-85.
    20.
  20. Fongmoon, D., Lalitwongsa, S., Keyoonwong, W., Nakong, M. and Iamsaard, S., 2013. Antioxidant activity and cytotoxicity of bitter melon (Momordicacharantia L.) Extract cultured in lampang Thailand. International Journal of Science, 10(2): 18-25.
    21.
  21. Frankel E.N., 1999 .Recent advances in lipid oxidation. Journal of the Science of Food and Agriculture, 54: 495-511.
    22.
  22. Fidrianny, I., Ramadhani, S. and Komar, R., 2015. In vitro Antioxidant capacities of three organs of bitter gourd (Momordica charantia L.) form west Java-Indonesia using DPPH and FRAP assays. International Journal ofPharmacognosy and Phytochemical Research, 7(5): 1034-1041.
    23.
  23. Ghorbani Ghozhdi, H., 2013. Introduction to the foundations of medicinal herbs, spices and aromatic plants. Shahroud University Publication, Shahroud, 587p.
    24.
  24. Ghous, T., Aziz, N., Mehmood, Z. and Andleeb, S., 2015. Comparative study of antioxidant, metal chelating and antiglycation activities of Momordica charantia flesh and pulp fractions. Pakistan Journal of Pharmaceutical Sciences, 28(4): 1217-1223.
    25.
  25. Greige-Gerges, H., Khalil, R.A., Mansour, E.A., Magdalou, J., Chahine, R. and Ouaini, N., 2007. Cucurbitacins from Ecballium elaterium juice increase the binding of Bilirubin and Ibuprofen to albumin in human plasma. Chemico-Biological Interactions, 169(1): 53-62.
    26.
  26. Hamissou, M., Smith, A.C., Carter, R.E. and Triplett, J.K., 2013. Antioxidative properties of Bitter gourd (Momordica charantia) and Zucchini (Cucurbita pepo). Emirates Journal of Food and Agriculture, 25(9): 641.
    27.
  27. Heidari, M. and Mobaseri Moghadam, M., 2012. Effect of rate and time of nitrogen application on fruit yield and accumulation of nutrient elements in Momordicacharantia.Journal of the Saudi Society of Agriculture Sciences, 11(2): 129-133.
    28.
  28. Herch, W., Kallel, H. and Boukhchina, S., 2014. Physicochemical properties and antioxidant activity of Tunisian Date Palm oil as affected by different extraction methods. Food Science and Technology, 34(3): 464-470. 
    29.
  29. Horax, R., Hettiarachchy, N. and Chen,P., 2010. Extraction, quantification, andantioxidant activities of phenolics frompericarp and seeds of bitter melons (Momordicacharantia) harvested atthree maturity stages (immature, mature,and ripe). Journal of Agricultural andFood Chemistry, 58(7): 4428-4433.
    30.
  30. Hugh C.C. and Whitaker, T. W. 1961. History and Distribution of the Cultivated Cucurbits in the Americas. American Antiquity, 26(4): 469-485.
    31.
  31. Husein, A.I., Ali-Shtayeh, M.S., Jondi, W.J., Zatar, N.A., Abu-Reidah, I.M. and Jamous, R.M.,2014. In vitro antioxidant and antitumor activitiesof six selected plants used in the Traditional Arabic Palestinianherbal medicine. Pharm Biol, 52: 1249–1255.
    32.
  32. İncedayi, B., Tamer, C.E., Sinir, G.O., Suna, S. and Çopur, O.U., 2016. Impact of different drying parameters on color, β-carotene, antioxidant activity and minerals of Apricot (Prunusarmeniaca L.). Food Science and Technology, 36(1): 171-178. 
    33.
  33. Ismail, H.I., Chan, K.W., Mariod, A.A., and Ismail, M., 2010. Phenolic content and antioxidant activity of Cantaloupe (Cucumis melo) methanolic extracts. Food Chemistry, 119: 643–647.
    34.
  34. Jaradat, N., Jodehb, S., Rinnob, T., Kharoof, M., Zaida, A.N., and Hannon, M., 2012. Determination the presence of phytomelin in Ecballiumelaterium to approve its folk uses .International Journal of Pharmacy and Pharmaceutical Sciences, 4(2): 233-237.
    35.
  35. Katalinic, V., MIlos, M., Kulisic, T., Jukic, M., 2006. Screening of 70 medical plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94: 550-577.
    36.
  36. Kris-Ethertonm, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E. and Hilpert, K.F., 2002. Bioactive compounds in foods: their role in the prevention of cardiovasculardisease and cancer. American Journal of Medicine, 113: 71-88.
    37.
  37. Kubola, J. and Siriamornpun, S., 2008. Phenolic contents and antioxidant activities of Bitter gourd (Momordica charantia L.) leaf stem and fruit fraction extracts in vitro. Food Chemistry, 110: 881-890.
    38.
  38. Madrau, M.A., Pisicopo, A., Sanguinetti, A.M., Del Caro, A., Poiana, M., Romeo, F.V. and Piga, A., 2009. Effect of drying temperature on polyphenolic content and antioxidant activity of apricots. European Food Research and Technology, 228: 441-448. 
    39.
  39. Mathew, S. and Abraham T.E., 2006. In vitro antioxidant activity andscavenging effects ofCinnamomumverum leaf extract assayed by different methodologies. Food Chem Toxicol: 44:198-206.
    40.
  40. Moayyed Amini, R., Ghanbari,A.R., Staji, A.,Shokouhian, A.A. and Elhami, B., 2017. Assessment of phisico-chemical and genetic variation by ISSR markers in squirting cucumber (Ecballiumelaterium) Ardebil province. M.Sc.thesis, Faculty of Agriculture and Natural Resources,Department of Horticultural Sciences, University of Mohaghegh Ardebili, Ardebil.
    41.
  41. Nile, S.H., and Park, S.W.,2013. Total phenolics, antioxidant and xanthine oxidase inhibitory activity of three colored onions (Alliumcepa L.). Front Life Sci, 7: 224–228.
    42.
  42. Nile, S.H., and Park, S.W., 2015. Chromatographic analysis, antioxidant,anti-inflammatory and xanthine oxidase inhibitory activities ofginger extracts and its reference compounds. Ind Crop Prod,70: 238–244.
    43.
  43. Nile, S.H., Nile, A.S., and   Keum,Y.S., 2017. Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. Biotech, 7: 76-86.
    44.
  44. Othman, A., Ismail, A., Ghani, N. A. and Adenan, I., 2007. Antioxidant capacity and phenolic content of Cocoa beans. Food Chemistry, 100, 1523–1530.
    45.
  45. Phillips, K.M., Ruggio, D.M and . Ashraf-Khorassani, M,. 2005. Phytosterol composition of nuts and seeds commonly consumed in the United States. Journal of Agricultural and Food Chemistry, 53: 9436-9445.
    46.
  46. Patel, S., Patel, T., Parmar, K., Patel, B., and Patel, P., 2011. Evaluation ofantioxidant activity, phenol and flavonoidcontents of Momordica charantia Linnfruit. Advance Research inPharmaceuticals and Biologicals, 1: 120-129.
    47.
  47. Rabrenovic, C.C., Dimic, E.B., Novakovic, M.M., Tesevic, V.Vand . Basic, Z.N,. 2014. Themost important bioactive components of cold pressed oil from different pumpkin(Cucurbitapipo L.) seeds. Food Sci Technol, 55: 521-527.
    48.
  48. Sabudak, T,. 2007. Fatty acid composition of seed and leaf oils of pumpkin. Walnut,almond, maize, sunflower and melon. Chem Nat Compounds, 43: 465-467.
    49.
  49. Salhab, A.S., 2013. Human exposure to fruit juice: fatal toxicity and possible remedy Ecballiumelaterium. Pharmacology and Pharmacy, 4(05): 447-450.
    50.
  50. Sargin, S.A., Akcicek, E. and Selvi, S., 2013. An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. Journal of Ethnopharmacology, 150(3): 860-874.
    51.
  51. Shahidi, F.  andNaczk, M., 2004. Phenolics in food and nutraceuticals. Boca Raton, FL:CRC Press. United States, 576 p.
    52.
  52. Shan, B., Xie, J.H., Zhu, J.H .and Peng Y., 2012. Ethanol modified supercritical carbon dioxide extraction of flavonoids from Momordica charantia L. and its antioxidant activity. Food and Bioproducts Processing ,90:579-587.
    53.
  53. Smith, B.D., 1997. The initial domestication of Cucurbitapepo in the Americas10,000 years ago. Science, 276: 932-934.
    54.
  54. Stevenson, D.G., Eller, F.J., Wang, L., Jane, J.L.,and  Wang, T., 2007. Oil and tocopherole content and composition of pumpkin seed oil in 12 cultivars. Journal of Agricultural and Food Chemistry, 55: 4005-4013.
    55.
  55. Tachakittirungrod, S., Okonogi, S. and Chowwanapoonpohn, S., 2007. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidantaction of Guava leaf extract. Food Chemistry, 103(2): 381-388.
    56.
  56. The´riault, V., Bernatchez, L., Dodson, J.J., 2007. Mating system and individual reproductive success of sympatric anadromous and resident brook charr, Salvelinus fontinalis, under natural conditions. Behavioral Ecology and Sociobiology, 62: 51-65.
    57.
  57. Toker, G., Memisoglu, M., Toker, M.C., and Yesilada, E., 2003. Callus formation and cucurbitacin B accumulation in Ecballiumelaterium callus cultures.Fitoterapia, 74(7-8): 618-623. 
    58.
  58. WHO. [No authors listed]., 1993. Evaluation of certain food additives and contaminants.Forty-first report of the Joint FAO/WHO Expert Committee on Food Additives.World Health Organ Tech Rep Ser, 837: 1-53.
    59.
  59. Wakimoto, N., Yin, D., O'Kelly, J., Haritunians, T., Karlan, B., Said J., Xing, H., and Koeffler, H.P., 2008.Cucurbitacin B has a potent antiproliferative effect on breast cancer cells in vitro and in vivo. Cancer Sci, 99(9):1793-1797.
    60.
  60. Yanishlieva-Maslarova, N.V., 2001. Inhibiting oxidation. In J. Pokorny, N., Yanishlieva, & M.H. Gordon (Eds.), Antioxidants in food: Practical applications(pp. 22–70). England, 400 p.
    61.

 

 

 

 

 

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