The assessment of Klebsiella pneumonia causing urinary tract infection in hospitalized patients
Subject Areas : Biotechnology and Applied Microbiology journal
Keywords: Urinary Tract Infection, Klebsiella pneumonia, PFGE, Catheterization,
Abstract :
Urinary tract infection (UTI) is the most common nosocomial infection. Microorganisms causing UTI are resistant to most antibiotics. Klebsiella pneumonia is one of the major causes of UTI among catheterized patients and their antibiotic resistance leads to medical implications associated with UTIs. The aim of this study was to assess the prevalence of K. pneumonia strains, to identify their invitro susceptibility to antimicrobial agents and to determine genetic diversity of them. Urine samples were cultured. The 105 CFU/ml cultures were assumed as positive. Antibiotic susceptibility of each isolate was assessed according to CLSI guidelines. In order to analyze bacterial genotypic diversity, pulsed- field gel electrophoresis (PFGE) was performed.116 out of 163 urine samples were positive. Klebsiella species were placed in second step of the most frequent isolates. Antibiotic sensitivity was observed against most of antimicrobial agents. Some of strains were genetically similar.This study showed that Klebsiella was present with relatively high number, but most of these isolates had acceptable sensitivity to most of the antibiotic agents. Also, relative pattern observed in 3 types revealed that these strains originated from hospital environment or they are common types which living in a major portion of population
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