Effects of oral nicotine on the developing crown-romp lengths and brain rat embryos
Subject Areas : Biotechnology and Applied Microbiology journal
Keywords: Embryo, Hippocampus, nicotine, Wistar Rat, Crown-Romp Lengths,
Abstract :
Tobacco exposure is not only a health concern for adults, however. It has also been shown to exert deleterious effects on the health of the fetus, newborn, child, and adolescent .The present study evaluated the possible role of nicotine on the developing rat fetus that include developing brain and crown-romp lengths fetus. Wistar rats (250-300 g) were used in this study. After pregnancy, the animals were divided into control and nicotine (0.05 mg in tap water) groups. On 14th embryonic day, the rats were killed and their embryos were surgically removed, washed, crown-romp lengths were measuredand were fixed in formalin 10%. Then tissue processing, cutting and Hematoxylin- Eosin (H&E) staining were preformed. The samples were evaluated using light microscope and MOTIC software for changes in hippocampus layer cell numbers. Data were analyzed using SPSS software version 9.01.The crown-romp length of embryos was decreased in the nicotine group. In addition, the number of hippocampus cell layer was decrease.It seems that nicotine induced their influence on embryo development via different pathways and it has toxic effect on the brain
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