Studing the effect of fetalliver extract on,umbilical cordmesenchymal stemcells
Subject Areas : Biotechnology and Applied Microbiology journal
Keywords: in vitro, Differentiation, Mesenchymal Stem Cells (MSCs), Hepatocyte, glycogen synthesis,
Abstract :
Stem cells have various potential of differentiation in several levels of an Embryo development. These cells can participate in regeneration and recovery of injured tissues. They have ability of self -renewal and this means these cells can produce similar cells for long period of time.In consequence of this issue they are considered suitable candidate for treatment of liver diseases. Mesenchymal stem cells are described as a kind of non-hematopoetic stem cells that resident in bone marrow and other skeleton tissues.The purpose of present study was to examine the effect of liver extract on umbilical cord-derived NMIR mouse Mesenchymal Stem Cells into Hepatocyte-like cells and with this protocol of differentiation we finally got hepatocyte-like cells. MSCs were isolated from NMRI mouse on days 14-17 and cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% FBS (Fetal Bovine Serum).we investigate the differentiation potential of mouse Mesenchymal stem cells into hepatocytes in vitro by using liver extract every 4 days.We cultured Mesenchymal stem cells without additional factors or late differential markers of hepatic development. Our results have showed Mesenchymal stem cells after 30 days were changed to hepatocyte-like cells. These cells have multidimensional morphology of hepatocyte-like cells with 1 or 2 obvious nuclei.Periodic Acid –Schiff (PAS)test used for detecting the amount of glycogen synthesis in differentiated Mesenchymal stem cells toward hepatocytes-like cells and the colour of red confirm this differentiation.
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